Allele Dmel\spas^Scer\UAS.cTa

General Information
Symbol	Dmel\spas^Scer\UAS.cTa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Trotta	FlyBase ID	FBal0158619
Feature type	allele	Associated gene	Dmel\spas

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Trotta et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a spas cDNA. (Trotta et al., 2004)
Carried in construct	P{UAS-spas.T} (Trotta et al., 2004, Jin et al., 2009, Stramer et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	cell polarity defective, with Scer\GAL4^srp.Hemo (Stramer et al., 2010) lethal, with Scer\GAL4^elav-C155 (Trotta et al., 2004) lethal, with Scer\GAL4^αTub84B.PL (Trotta et al., 2004) neurophysiology defective, with Scer\GAL4^elav-C155 (Trotta et al., 2004)
Phenotype Manifest In
	cytoskeleton, with Scer\GAL4^srp.Hemo (Stramer et al., 2010) hemocyte, with Scer\GAL4^srp.Hemo (Stramer et al., 2010) microtubule, with Scer\GAL4^C57 (Jin et al., 2009) microtubule, with Scer\GAL4^srp.Hemo (Stramer et al., 2010) neuromuscular junction \| larval stage, with Scer\GAL4^elav-C155 (Trotta et al., 2004)
Detailed Description
	Statement Reference Expression of spas[Scer\UAS.cTa] in hemocytes under the control of Scer\GAL4[srp.Hemo] prevents assembly of a normal microtubule cytoskeleton. Early developmental dispersal of hemocytes along the ventral midline in these embryos is delayed; nonetheless, most hemocytes found their way to the ventral midline by stage 14. Despite being unable to form a microtubule arm, these cells are still able to respond to wound stimuli, although with less efficiency than wild-type. These cells fail to maintain directional persistence (i.e. they do not go straight to the wounded area) but do migrate faster than in wild-type. This results in a slightly reduced number of spas-expressing cells present at a wound 1 hour after ablation compared to wild-type. From stage 15 onwards spas[Scer\UAS.cTa]-expressing hemocytes (where spas[Scer\UAS.cTa] is under the control of Scer\GAL4[srp.Hemo]) fail to disperse from the ventral midline. These hemocytes are unable to polarize and remain in close contact with each other at stages when they would ordinarily be exhibiting contact repulsion from one another. While wild-type hemocytes are rarely in contact with one another for more than 10 minutes, spas[Scer\UAS.cTa]-expressing hemocytes frequently retain contacts for more than 15 minutes. (Stramer et al., 2010) Expression of spas[Scer\UAS.cTa] under the control of Scer\GAL4[C57] severs microtubules. (Jin et al., 2009) Expression of spas^Scer\UAS.cTa under the control of Scer\GAL4^elav-C155 or Scer\GAL4^αTub84B.PL generally results in 100% lethality during embryonic or very early larval stages (unless a particularly weakly expressing P{UAS-spas.T} transgene is used). Expression of spas^Scer\UAS.cTa under the control of Scer\GAL4^elav-C155 (using a weakly expressing P{UAS-spas.T} transgene that allows full viability) does not have a significant effect on the total gross synaptic area at the larval neuromuscular junction. However, these animals show a significant reduction in mean excitatory junctional current (EJC) amplitude at the larval NMJ compared to controls (47.3 +/- 8.5 nA compared to 85.3 +/- 3.8 nA). This decrease in mean EJC amplitude can be rescued if the animals are pretreated with 50μM taxol before recording the current. (Trotta et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference When tbce[Scer\UAS.cJa] and spas[Scer\UAS.cTa] are co-expressed under the control of Scer\GAL4[C57], the microtubule phenotype in the muscles is similar to that of animals expressing spas[Scer\UAS.cTa] alone under the control of Scer\GAL4[C57]. When tbce[dsRNA.Scer\UAS.cJa] and spas[Scer\UAS.cTa] are co-expressed under the control of Scer\GAL4[C57], the microtubule phenotype in the muscles is similar to that of animals expressing spas[Scer\UAS.cTa] alone under the control of Scer\GAL4[C57]. (Jin et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	spas^Scer\UAS.cTa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Trotta (Trotta et al., 2004)
Secondary FlyBase IDs

References ( 3 )
Research paper	Stramer et al., 2010, J. Cell Biol. 189(4): 681--689 Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo. [FBrf0210787] Jin et al., 2009, Development 136(9): 1571--1581 Drosophila Tubulin-specific chaperone E functions at neuromuscular synapses and is required for microtubule network formation. [FBrf0209976] Trotta et al., 2004, Curr. Biol. 14(13): 1135--1147 The hereditary spastic paraplegia gene, spastin, regulates microtubule stability to modulate synaptic structure and function. [FBrf0179488]

Allele Dmel\spasScer\UAS.cTa

Allele Dmel\spas^Scer\UAS.cTa