Allele Dmel\pAbp^EY11561

General Information
Symbol	Dmel\pAbp^EY11561	Species	D. melanogaster
Name		FlyBase ID	FBal0158843
Feature type	allele	Associated gene	Dmel\pAbp

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Gene Disruption Project members, 2001-)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:14,028,250..14,028,250 (Gene Disruption Project members, 2001-, Cziko et al., 2009, Pertceva et al., 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{EPgy2}pAbp^EY11561 (Gene Disruption Project members, 2001-, Cziko et al., 2009, Pertceva et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	cytokinesis defective (with pAbp^k10109) (Pertceva et al., 2010) female fertile (with Df(2R)Pcl7B) (Blagden et al., 2009) male sterile (with Df(2R)Pcl7B) (Blagden et al., 2009) meiotic cell cycle defective (with Df(2R)Pcl7B) (Blagden et al., 2009) viable (with Df(2R)Pcl7B) (Blagden et al., 2009)
Phenotype Manifest In
	centriole & spermatocyte (with Df(2R)Pcl7B) (Blagden et al., 2009) class IV dendritic arborizing neuron \| somatic clone, with Scer\GAL4^Act.PU (Cziko et al., 2009) elongation stage spermatid (with pAbp^k10109) (Pertceva et al., 2010) mitochondrion (with pAbp^k10109) (Pertceva et al., 2010) Nebenkern (with pAbp^k10109) (Pertceva et al., 2010) nucleus (with pAbp^k10109) (Pertceva et al., 2010) onion stage spermatid (with pAbp^k10109) (Pertceva et al., 2010) spindle & spermatocyte (with Df(2R)Pcl7B) (Blagden et al., 2009)
Detailed Description
	Statement Reference pAbp[k10109]/pAbp[EY11561] heterozygotes exhibit abnormalities in spermatogenesis. these include absence of meiotic cytokinesis (in 5% of cases), resulting in formation of polyploid spermatids and defects in spermatid elongation (in approximately 100% of cases). Nuclei fail to elongate, keep a round shape and are dispersed along the cyst instead of grouping at the head of the cyst. Nebenkern DAPi staining (for DNA) appears to be non-uniform in these mutants, reflecting the effect of the trans-heterozygous mutants on mitochondrial DNA organization in at least 60% of cases. There appears to be no difference between the trans-heterozygous mutant and controls, showing that mutant mitochondrial membranes keep their integrity, but in the mutant, the onion-stage Nebenkern looks larger than in controls, and its morphology differs from a perfect sphere. Examination with an electron microscope reveals abnormal mitochondrial membranes at onion-stage spermatids in the trans-heterozygous mutant. These mitochondria also exhibit defective attachment to axonemes in the elongating spermatids. (Pertceva et al., 2010) pAbp[EY11561]/Df(2R)Pcl7B spermatocytes show defects in meiosis; some spermatocytes have short spindles, and premature separation of centrioles is seen in some cases. (Blagden et al., 2009) Over-expression of pAbp[EY11561] reduces dendrite branching, and alters dendritic morphology in larval class IV sensory neurons (induced under the control of Scer\GAL4[Act.PU]). Total dendrite length is not significantly reduced in these neurons. (Cziko et al., 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	20684 y¹ w^67c23; P{EPgy2}pAbp^EY11561
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	pAbp^EY11561 (Blagden et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Pertceva et al., 2010, Cell Biol. Int. 34(10): 991--996 The role of Drosophila hyperplastic discs gene in spermatogenesis. [FBrf0211767] Blagden et al., 2009, Dev. Biol. 334(1): 186--197 Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development. [FBrf0208825] Cziko et al., 2009, Genetics 182(4): 1051--1060 Genetic Modifiers of dFMR1 Encode RNA Granule Components in Drosophila. [FBrf0208636]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]

Allele Dmel\pAbpEY11561

Allele Dmel\pAbp^EY11561