Allele Dmel\dia^{CA.Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\dia^{CA.Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0159328
Feature type	allele	Associated gene	Dmel\dia

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Somogyi and Rorth, 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of dia cDNA SD13607 in which the N-terminal 449 amino acids have been replaced by a short sequence encoding three T:Ivir\HA1 tags, and the C-terminal amino acids 1029-1091 (the predicted autoinhibitory domain) have been removed. Encodes an activated form of dia. (Somogyi and Rorth, 2004)
Carried in construct	P{UAS-dia.CA} (Warner and Longmore, 2009, Somogyi and Rorth, 2004, Janody and Treisman, 2006, Matusek et al., 2006, Homem and Peifer, 2008, Massarwa et al., 2009, Bertet et al., 2009, Matusek et al., 2008, Schoen et al., 2010, Laplante et al., 2010, Homem and Peifer, 2009)
Tagged with	T:Ivir\HA1 (Somogyi and Rorth, 2004)
Cytology
Phenotypic Data
Phenotypic Class
	auditory perception defective, with Scer\GAL4^{CG13795-NP1245} (Schoen et al., 2010) auditory perception defective, with Scer\GAL4^JO15 (Schoen et al., 2010) auditory perception defective, with Scer\GAL4^NP6303 (Schoen et al., 2010) auditory perception defective, with Scer\GAL4^ph-p-NP1046 (Schoen et al., 2010) cell shape defective \| extended germ band stage (Bertet et al., 2009) lethal \| pupal stage, with Scer\GAL4^ph-p-NP0761 (Schoen et al., 2010) neuroanatomy defective, with Scer\GAL4^elav-C155 (Matusek et al., 2008) partially lethal - majority die, with Scer\GAL4^ph-p-NP1046 (Schoen et al., 2010)
Phenotype Manifest In
	amnioserosa, with Scer\GAL4^c381 (Homem and Peifer, 2008) amnioserosa, with Scer\GAL4^en-e16E (Homem and Peifer, 2008) border follicle cell, with Scer\GAL4^slbo.2.6 (Somogyi and Rorth, 2004) commissure, with Scer\GAL4^elav-C155 (Matusek et al., 2008) connective, with Scer\GAL4^elav-C155 (Matusek et al., 2008) ectoderm \| extended germ band stage (Bertet et al., 2009) embryonic/larval dorsal trunk, with Scer\GAL4^btl.PS (Matusek et al., 2006) embryonic/larval dorsal trunk, with Scer\GAL4^en-e16E (Laplante et al., 2010) embryonic/larval dorsal trunk, with Scer\GAL4^prd.RG1 (Laplante et al., 2010) embryonic epidermis, with Scer\GAL4^en-e16E (Homem and Peifer, 2008, Laplante et al., 2010) embryonic epidermis, with Scer\GAL4^prd.RG1 (Homem and Peifer, 2008, Laplante et al., 2010) epithelial cell \| somatic clone, with Scer\GAL4^Act5C.PI (Warner and Longmore, 2009) organism \| dorsal closure stage, with Scer\GAL4^c381 (Homem and Peifer, 2008) presumptive embryonic/larval central nervous system, with Scer\GAL4^elav-C155 (Matusek et al., 2008) tracheal fusion cell, with Scer\GAL4^btl.PS (Matusek et al., 2006)
Detailed Description
	Statement Reference Embryos expressing dia[CA.Scer\UAS.T:Ivir\HA1] under the control of either Scer\GAL4[en-e16E] or Scer\GAL4[prd.RG1] have deep epidermal grooves. The epidermis appears to impinge upon the dorsal trunk in these embryos and the underlying trachea are more convoluted than wild type. No breaks in the tracheal lateral trunk are seen in these embryos. (Laplante et al., 2010) Flies expressing dia[CA.Scer\UAS.T:Ivir\HA1] in the Johnston's organ under the control of Scer\GAL4[ph-p-NP1046], Scer\GAL4[CG13795-NP1245], Scer\GAL4[NP6303] or Scer\GAL4[JO15] display an impaired response to sound. A significant reduction in sound-evoked potentials compared with wild-type and parental controls is observed in these animals. (Schoen et al., 2010) Expression of dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[mat.αTub67C.T:Hsim\VP16] results in a collapse of the epithelium, and the apical cell surfaces are reduced in size in embryos at germ-band extension. (Bertet et al., 2009) Expression of dia[CA.Scer\UAS.T:Ivir\HA1] in pupal eye MARCM clones does not affect the adherens junctions. dia[CA.Scer\UAS.T:Ivir\HA1] expressing cells develop a rounded morphology, especially primary pigment epithelial cells. (Warner and Longmore, 2009) Expression of dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[en-e16E] in individual amnioserosa cells induces premature apical constriction in these cells, and neighbouring wild-type cells become elongated. Cortical F-actin is increased in the cells that express dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[en-e16E]. Expression of dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[c381] in all amnioserosal cells results in all the cells becoming apically smaller and uniformly round, suggesting that they all attempt to constrict simultaneously. Cortical F-actin is increased in the amnioserosa cells. The network of apical myosin filaments that is normally seen in wild-type cells become reorganised into stress-fibre-like bundles parallel to the epithelium. Dorsal closure is substantially slowed in embryos expressing dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[c381] compared to wild type. Most of the embryos do complete dorsal closure, but have defects in cell-cell matching as the two epidermal sheets meet. Segmental grooves persist much longer than normal in embryos expressing dia[CA.Scer\UAS.T:Ivir\HA1] under the control of either Scer\GAL4[en-e16E] or Scer\GAL4[prd.RG1] and become very deep. Epidermal cells expressing dia[CA.Scer\UAS.T:Ivir\HA1] under the control of either Scer\GAL4[en-e16E] are wider in the anterior/posterior axis and shorter in the dorsal/ventral axis than neighbouring wild-type cells. Epidermal cells expressing dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[en-e16E] have elevated cortical F-actin levels compared to wild type. Epidermal cells expressing dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[prd.RG1] have higher levels of cortical myosin compared to wild-type neighbouring cells, with myosin being especially enriched at the dorsal/ventral cell borders. (Homem and Peifer, 2008) Expression of dia[CA.Scer\UAS.T:Ivir\HA1] pan-neuronally under the control of Scer\GAL4[elav-C155] results in disruption of CNS fasciculation and thinning of commissures and breaks in connectives can be observed. (Matusek et al., 2008) Overexpression of dia^{CA.Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^btl.PS, leads to fusion defects in the dorsal trunk, constrictions at the fusion cells, widening at other areas of the dorsal trunk and impairments of terminal branch differentiation. (Matusek et al., 2006) Border cell migration is blocked by expression of dia^{CA.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^slbo.2.6. (Somogyi and Rorth, 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement Reference Scer\GAL4^c381, dia^{CA.Scer\UAS.T:Ivir\HA1} has amnioserosa phenotype, non-suppressible by zip¹/zip¹ (Homem and Peifer, 2008) Scer\GAL4^en-e16E, dia^{CA.Scer\UAS.T:Ivir\HA1} has embryonic epidermis phenotype, non-suppressible by zip¹/zip¹ (Homem and Peifer, 2008)
NOT Suppressor of
Statement Reference Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, dia^{CA.Scer\UAS.T:Ivir\HA1} is a non-suppressor of adherens junction \| somatic clone phenotype of Rho1^72F (Warner and Longmore, 2009) Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, dia^{CA.Scer\UAS.T:Ivir\HA1} is a non-suppressor of adherens junction \| somatic clone phenotype of Rho1^72O (Warner and Longmore, 2009)
Additional Comments
Genetic Interactions
Statement Reference Rho1[72F] clones expressing dia[CA.Scer\UAS.T:Ivir\HA1] still exhibit disrupted adherens junctions. Rho1[72O] clones expressing dia[CA.Scer\UAS.T:Ivir\HA1] still exhibit disrupted adherens junctions. (Warner and Longmore, 2009) The cell shape changes that are seen in epidermal cells of embryos expressing dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[en-e16E] are still seen in a zip[1] homozygous background. The cell shape changes that are seen in amnioserosa cells of embryos expressing dia[CA.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[c381] are still seen in a zip[1] homozygous background. (Homem and Peifer, 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	27616 w^*; P{UAS-dia.CA}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	dia^{CA.Scer\UAS.T:Ivir\HA1} Dia^CA (Matusek et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Laplante et al., 2010, Dev. Dyn. 239(9): 2509--2519 Echinoid regulates tracheal morphology and fusion cell fate in Drosophila. [FBrf0211771] Schoen et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(30): 13396--13401 Increased activity of Diaphanous homolog 3 (DIAPH3)/diaphanous causes hearing defects in humans with auditory neuropathy and in Drosophila. [FBrf0211399] Bertet et al., 2009, Development 136(24): 4199--4212 Repression of Wasp by JAK/STAT signalling inhibits medial actomyosin network assembly and apical cell constriction in intercalating epithelial cells. [FBrf0209348] Homem and Peifer, 2009, Mol. Biol. Cell 20(24): 5138--5155 Exploring the Roles of Diaphanous and Enabled Activity in Shaping the Balance between Filopodia and Lamellipodia. [FBrf0209542] Massarwa et al., 2009, Dev. Cell 16(6): 877--888 Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous. [FBrf0208270] Warner and Longmore, 2009, J. Cell Biol. 185(6): 1111--1125 Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia. [FBrf0208183] Homem and Peifer, 2008, Development 135(6): 1005--1018 Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis. [FBrf0204461] Matusek et al., 2008, J. Neurosci. 28(49): 13310--13319 Formin proteins of the DAAM subfamily play a role during axon growth. [FBrf0206320] Janody and Treisman, 2006, Development 133(17): 3349--3357 Actin capping protein alpha maintains vestigial-expressing cells within the Drosophila wing disc epithelium. [FBrf0193788] Matusek et al., 2006, Development 133(5): 957--966 The Drosophila formin DAAM regulates the tracheal cuticle pattern through organizing the actin cytoskeleton. [FBrf0190308] Somogyi and Rorth, 2004, Dev. Cell 7(1): 85--93 Evidence for tension-based regulation of Drosophila MAL and SRF during invasive cell migration. [FBrf0179449]

Allele Dmel\diaCA.Scer\UAS.T:Ivir\HA1

Allele Dmel\dia^{CA.Scer\UAS.T:Ivir\HA1}