Individuals that both express cacUAS.EGFP under the control of Scer\GAL4elav-C155 and are heterozygous for RyRE4340K show almost no baseline hyperexcitability of larval NMJs (i.e extra EPSP discharges).
The expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Toll-6-D42, Scer\GAL4RapGAP1-OK6, Scer\GAL4GMR75C05 or Scer\GAL4GMR12A09, but not of Scer\GAL4GMR82E12 or Scer\GAL4GMR15A04, partially suppresses the decreased locomotor capacity of TBPHG2 third instar larvae in crawling assays; expression of under the control of either Scer\GAL4RapGAP1-OK6 or Scer\GAL4GMR75C05 also partially suppresses the associated slower and less frequent peristaltic waves. None of these cacScer\UAS.T:Avic\GFP-EGFP expression conditions rescued the adult lethality of TBPHG2 mutants.
The expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4RapGAP1-OK6, but not of Scer\GAL4GMR75C05, suppresses the decreased frequency, but not the decreased amplitude, of spontaneous miniature endplate potentials in the neurotransmission across the TBPHG2 larval neuromuscular junction. Scer\GAL4RapGAP1-OK6- and Scer\GAL4GMR75C05-driven expression suppresses the decrease in motor bursts and the increased delay between A7 and A2 motor bursts observed TBPHG2 mutant larvae.
Expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4Appl.G1a or Scer\GAL4Toll-6-D42 (but not Scer\GAL4ChAT.PU) significantly suppresses locomotor defects (but not pupal lethality) in TBPHG2/TBPHG2 third instar larvae.
Expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4103.3 in a Ca-α1Tdel background results in low voltage activated, maximum total calcium current and sustained high voltage activated calcium currents with normal activation voltages but reduced amplitudes compared to controls in the adult MN5 neuron.
cacScer\UAS.T:Avic\GFP-EGFP; Scer\GAL4elav.PLu fails to suppress the increase in bouton number or the reduction in evoked excitatory junction potentials (EJPs) seen at neuro-muscular junctions in stjk10814/Df(2R)CX1 third instar larvae. However, the reduction in mini (spontaneous) EJPs seen at these junctions is suppressed.
The reduced excitatory junctional potential amplitude seen at the third instar larval neuromuscular junction in stj1/Df(2R)Exel7128 and stj2/Df(2R)Exel7128 animals is partially suppressed by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155.
The hyperexcitability seen in recordings from muscles 6/7 of dissected third instar stj1/Df(2R)Exel7128 larvae with intact ventral nerve cords at elevated temperature (36[o]C) is reduced by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155; these animals rarely show activity bursts lasting 30 seconds or more.