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General Information
Symbol
Dmel\cacUAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0159426
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
CacGFP, UAS-Cac-GFP, UAS-Cacophony-GFP, CAC-GFP, UAS-cac1-EGFP, uas-cacophony-eGFP, UAS-cacGFP, UAS-CacGFP, UAS-cac-eGFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of cac tagged with EGFP is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

cacexon7Δ mutant adult male show significantly reduced levels of activity (as measured in a bioactivity recorder), as compared to controls; the expression of cacUAS.EGFP under the control of Scer\GAL4Appl.G1a rescues this defect, to the point of even inducing a significantly higher activity than controls.

Adults expressing cacUAS.EGFP under the control of Scer\GAL4elav-C155 do not show significant changes in lifespan. In CNS-removed larvae, NMJ neurotransmission does not show any significant changes in mEPSP frequency, amplitude and rise time, or in EPSP amplitude and quantal content, as compared to controls; larger spontaneous events are slower despite the absence of gigantic spontaneous events.

The expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav.PU, Scer\GAL4RapGAP1-OK6 or Scer\GAL4GMR75C05 does not lead to third instar larval locomotor defects in crawling assays, as compared to controls.

Over-expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4elav-C155 does not significantly alter third instar larval crawling behavior.

Expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4elav-C155 has no effect on baseline synaptic transmission at the larval neuromuscular junction (NMJ).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

cacUAS.EGFP/Scer\GAL4Toll-6-D42 is a non-suppressor of lethal phenotype of TBPHG2

Scer\GAL4GMR75C05/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

Scer\GAL4GMR12A09/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

Scer\GAL4GMR82E12/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

Scer\GAL4GMR15A04/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Individuals that both express cacUAS.EGFP under the control of Scer\GAL4elav-C155 and are heterozygous for RyRE4340K show almost no baseline hyperexcitability of larval NMJs (i.e extra EPSP discharges).

The expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Toll-6-D42, Scer\GAL4RapGAP1-OK6, Scer\GAL4GMR75C05 or Scer\GAL4GMR12A09, but not of Scer\GAL4GMR82E12 or Scer\GAL4GMR15A04, partially suppresses the decreased locomotor capacity of TBPHG2 third instar larvae in crawling assays; expression of under the control of either Scer\GAL4RapGAP1-OK6 or Scer\GAL4GMR75C05 also partially suppresses the associated slower and less frequent peristaltic waves. None of these cacScer\UAS.T:Avic\GFP-EGFP expression conditions rescued the adult lethality of TBPHG2 mutants.

The expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4RapGAP1-OK6, but not of Scer\GAL4GMR75C05, suppresses the decreased frequency, but not the decreased amplitude, of spontaneous miniature endplate potentials in the neurotransmission across the TBPHG2 larval neuromuscular junction. Scer\GAL4RapGAP1-OK6- and Scer\GAL4GMR75C05-driven expression suppresses the decrease in motor bursts and the increased delay between A7 and A2 motor bursts observed TBPHG2 mutant larvae.

Expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4Appl.G1a or Scer\GAL4Toll-6-D42 (but not Scer\GAL4ChAT.PU) significantly suppresses locomotor defects (but not pupal lethality) in TBPHG2/TBPHG2 third instar larvae.

Expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4103.3 in a Ca-α1Tdel background results in low voltage activated, maximum total calcium current and sustained high voltage activated calcium currents with normal activation voltages but reduced amplitudes compared to controls in the adult MN5 neuron.

Expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 does not restore homeostatic compensation at the neuromuscular junction in dysbe01028 larvae.

Expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4elav-C155 in a GluRIIASP16; ExnEY01953 double mutant background restores the NMJ synaptic homeostatic response to a significant degree.

cacScer\UAS.T:Avic\GFP-EGFP; Scer\GAL4elav.PLu fails to suppress the increase in bouton number or the reduction in evoked excitatory junction potentials (EJPs) seen at neuro-muscular junctions in stjk10814/Df(2R)CX1 third instar larvae. However, the reduction in mini (spontaneous) EJPs seen at these junctions is suppressed.

The defects in mean EJC amplitude seen at the neuromuscular junction in fusl1 larvae are not rescued by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav.PLu.

stj1/Df(2R)Exel7128 and stj2/Df(2R)Exel7128 flies expressing cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 have "on-off" transients in electroretinogram recordings.

The reduced excitatory junctional potential amplitude seen at the third instar larval neuromuscular junction in stj1/Df(2R)Exel7128 and stj2/Df(2R)Exel7128 animals is partially suppressed by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155.

The hyperexcitability seen in recordings from muscles 6/7 of dissected third instar stj1/Df(2R)Exel7128 larvae with intact ventral nerve cords at elevated temperature (36[o]C) is reduced by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155; these animals rarely show activity bursts lasting 30 seconds or more.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
cacScer\UAS.T:Avic\GFP-EGFP
cacUAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (37)