FB2025_01 , released February 20, 2025
Allele: Dmel\cacUAS.EGFP
Open Close
General Information
Symbol
Dmel\cacUAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0159426
Feature type
allele
Associated gene
Dmel\cac
Associated Insertion(s)
Carried in Construct
P{UAS-cac1-EGFP}
Also Known As
UAS-Cac-GFP, CacGFP, CAC-GFP, UAS-cac1-EGFP, UAS-Cacophony-GFP, uas-cacophony-eGFP, UAS-cacGFP, UAS-CacGFP, UAS-cac, UAS-cac-eGFP
Key Links
Transgenic product class
wild_type
(Kawasaki et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kawasaki et al., 2004)
Mutagen
in vitro construct
(Kawasaki et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-cac1-EGFP}
Cytology
Description

Expression of cac tagged with EGFP is governed by UASt regulatory sequences.

(Kawasaki et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
cac
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  amyotrophic lateral sclerosis type 10
      modeled by TBPHG2
      (Chang et al., 2014)
      ameliorates  amyotrophic lateral sclerosis
      modeled by TBPHG2
      (Lembke et al., 2017)
      ameliorates  frontotemporal dementia
      modeled by TBPHG2
      (Lembke et al., 2017)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      cacexon7Δ mutant adult male show significantly reduced levels of activity (as measured in a bioactivity recorder), as compared to controls; the expression of cacUAS.EGFP under the control of Scer\GAL4Appl.G1a rescues this defect, to the point of even inducing a significantly higher activity than controls.

      (Lembke et al., 2019)

      Adults expressing cacUAS.EGFP under the control of Scer\GAL4elav-C155 do not show significant changes in lifespan. In CNS-removed larvae, NMJ neurotransmission does not show any significant changes in mEPSP frequency, amplitude and rise time, or in EPSP amplitude and quantal content, as compared to controls; larger spontaneous events are slower despite the absence of gigantic spontaneous events.

      (Brusich et al., 2018)

      The expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav.PU, Scer\GAL4RapGAP1-OK6 or Scer\GAL4GMR75C05 does not lead to third instar larval locomotor defects in crawling assays, as compared to controls.

      (Lembke et al., 2017)

      Over-expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4elav-C155 does not significantly alter third instar larval crawling behavior.

      (Chang et al., 2014)

      Expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4elav-C155 has no effect on baseline synaptic transmission at the larval neuromuscular junction (NMJ).

      (Frank et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      cacUAS.EGFP/Scer\GAL4Toll-6-D42 is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4RapGAP1-OK6/cacUAS.EGFP is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR75C05/cacUAS.EGFP is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR12A09/cacUAS.EGFP is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4RapGAP1-OK6/cacUAS.EGFP is a suppressor | partially of abnormal neurophysiology | larval stage phenotype of TBPHG2

      (Lembke et al., 2017)

      cacUAS.EGFP/Scer\GAL4Toll-6-D42 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Chang et al., 2014)

      Scer\GAL4Appl.G1a/cacUAS.EGFP is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Chang et al., 2014)

      cacUAS.EGFP/Scer\GAL4elav.PLu is a suppressor | partially of abnormal neurophysiology phenotype of Df(2R)CX1/stjk10814

      (Dickman et al., 2008)

      Scer\GAL4elav-C155/cacUAS.EGFP is a suppressor | partially of lethal phenotype of stj1/Df(2R)Exel7128

      (Ly et al., 2008)

      Scer\GAL4elav-C155/cacUAS.EGFP is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of stj1/Df(2R)Exel7128

      (Ly et al., 2008)

      Scer\GAL4elav-C155/cacUAS.EGFP is a suppressor | partially of lethal phenotype of stj2/Df(2R)Exel7128

      (Ly et al., 2008)

      Scer\GAL4elav-C155/cacUAS.EGFP is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of stj2/Df(2R)Exel7128

      (Ly et al., 2008)
      NOT Suppressor of
      Statement
      Reference

      cacUAS.EGFP/Scer\GAL4Toll-6-D42 is a non-suppressor of lethal phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4RapGAP1-OK6/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR75C05/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR12A09/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR82E12/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR15A04/cacUAS.EGFP is a non-suppressor of lethal phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR75C05/cacUAS.EGFP is a non-suppressor of abnormal neurophysiology | larval stage phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR82E12/cacUAS.EGFP is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4GMR15A04/cacUAS.EGFP is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4Appl.G1a/cacUAS.EGFP is a non-suppressor of lethal - all die before end of P-stage phenotype of TBPHG2

      (Chang et al., 2014)

      cacUAS.EGFP/Scer\GAL4Toll-6-D42 is a non-suppressor of lethal - all die before end of P-stage phenotype of TBPHG2

      (Chang et al., 2014)

      Scer\GAL4ChAT.PU/cacUAS.EGFP is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of TBPHG2

      (Chang et al., 2014)

      Scer\GAL4elav-C155/cacUAS.EGFP is a non-suppressor of abnormal neurophysiology phenotype of Dysbe01028

      (Dickman and Davis, 2009)

      cacUAS.EGFP/Scer\GAL4elav.PLu is a non-suppressor of abnormal neurophysiology phenotype of fusl1

      (Long et al., 2008)

      Scer\GAL4Mhc.PW/cacUAS.EGFP is a non-suppressor of lethal phenotype of stj1/Df(2R)Exel7128

      (Ly et al., 2008)

      Scer\GAL4Mhc.PW/cacUAS.EGFP is a non-suppressor of lethal phenotype of stj2/Df(2R)Exel7128

      (Ly et al., 2008)
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Individuals that both express cacUAS.EGFP under the control of Scer\GAL4elav-C155 and are heterozygous for RyRE4340K show almost no baseline hyperexcitability of larval NMJs (i.e extra EPSP discharges).

      (Brusich et al., 2018)

      The expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Toll-6-D42, Scer\GAL4RapGAP1-OK6, Scer\GAL4GMR75C05 or Scer\GAL4GMR12A09, but not of Scer\GAL4GMR82E12 or Scer\GAL4GMR15A04, partially suppresses the decreased locomotor capacity of TBPHG2 third instar larvae in crawling assays; expression of under the control of either Scer\GAL4RapGAP1-OK6 or Scer\GAL4GMR75C05 also partially suppresses the associated slower and less frequent peristaltic waves. None of these cacScer\UAS.T:Avic\GFP-EGFP expression conditions rescued the adult lethality of TBPHG2 mutants.

      The expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4RapGAP1-OK6, but not of Scer\GAL4GMR75C05, suppresses the decreased frequency, but not the decreased amplitude, of spontaneous miniature endplate potentials in the neurotransmission across the TBPHG2 larval neuromuscular junction. Scer\GAL4RapGAP1-OK6- and Scer\GAL4GMR75C05-driven expression suppresses the decrease in motor bursts and the increased delay between A7 and A2 motor bursts observed TBPHG2 mutant larvae.

      (Lembke et al., 2017)

      Expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4Appl.G1a or Scer\GAL4Toll-6-D42 (but not Scer\GAL4ChAT.PU) significantly suppresses locomotor defects (but not pupal lethality) in TBPHG2/TBPHG2 third instar larvae.

      (Chang et al., 2014)

      Expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4103.3 in a Ca-α1Tdel background results in low voltage activated, maximum total calcium current and sustained high voltage activated calcium currents with normal activation voltages but reduced amplitudes compared to controls in the adult MN5 neuron.

      (Ryglewski et al., 2012)

      Expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 does not restore homeostatic compensation at the neuromuscular junction in dysbe01028 larvae.

      (Dickman and Davis, 2009)

      Expression of cacScer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4elav-C155 in a GluRIIASP16; ExnEY01953 double mutant background restores the NMJ synaptic homeostatic response to a significant degree.

      (Frank et al., 2009)

      cacScer\UAS.T:Avic\GFP-EGFP; Scer\GAL4elav.PLu fails to suppress the increase in bouton number or the reduction in evoked excitatory junction potentials (EJPs) seen at neuro-muscular junctions in stjk10814/Df(2R)CX1 third instar larvae. However, the reduction in mini (spontaneous) EJPs seen at these junctions is suppressed.

      (Dickman et al., 2008)

      The defects in mean EJC amplitude seen at the neuromuscular junction in fusl1 larvae are not rescued by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav.PLu.

      (Long et al., 2008)

      stj1/Df(2R)Exel7128 and stj2/Df(2R)Exel7128 flies expressing cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 have "on-off" transients in electroretinogram recordings.

      The reduced excitatory junctional potential amplitude seen at the third instar larval neuromuscular junction in stj1/Df(2R)Exel7128 and stj2/Df(2R)Exel7128 animals is partially suppressed by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155.

      The hyperexcitability seen in recordings from muscles 6/7 of dissected third instar stj1/Df(2R)Exel7128 larvae with intact ventral nerve cords at elevated temperature (36[o]C) is reduced by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155; these animals rarely show activity bursts lasting 30 seconds or more.

      (Ly et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      CacGFP
      (Liu et al., 2011, Fouquet et al., 2009)
      cacScer\UAS.T:Avic\GFP-EGFP
      cacUAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (41)