FlyBase Allele Report: Dmel\bai[1]

General Information

Symbol

Dmel\bai¹

Species

D. melanogaster

Name

FlyBase ID

FBal0159471

Feature type

allele

Associated gene

Dmel\bai

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A. Contains a G to A mutation within the splice site at the 3' end of intron 3, which forces the use of a cryptic splice site 10 nucleotides downstream, creating a mRNA with a 10 nt deletion.

(Bartoszewski et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:25,040,021..25,040,021 [+]

Nucleotide change:

G25040021A

Reported nucleotide change:

G?A

Comment:

Contains a G to A mutation within the splice site at the 3' end of intron 3, which forces the use of a cryptic splice site 10 nucleotides downstream, creating a mRNA with a 10 nt deletion, causing a frameshift.

(Bartoszewski et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Bartoszewski et al., 2004)

lethal

(Saleem et al., 2012)

lethal | maternal effect

(Bartoszewski et al., 2004)

male semi-sterile | recessive

(Bartoszewski et al., 2004)

partially lethal - majority die (with bai^d09741)

(Saleem et al., 2012)

partially lethal - majority live | recessive

(Bartoszewski et al., 2004)

Phenotype Manifest In

embryonic/first instar larval cuticle | dorsal | germline clone

(Bartoszewski et al., 2004)

wing vein | ectopic

(Bartoszewski et al., 2004)

Detailed Description

Statement

Reference

Embryos derived from homozygous bai¹ germ-line clones exhibit a weak ventralised phenotype. Homozygotes show reduced survival, eclosing at a rate between 0.1 to 20% of the expected number. Homozygous or hemizygous males show reduced fertility and females lay no eggs, although many developed late oocytes are present in their ovaries which appear morphologically normal. Wings of homozygous mutants grown at 19^oC have thickened veins that form delta-like broadenings close to the margin.

(Bartoszewski et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

bai¹ has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, enhanceable by tkv^SL144

(Bartoszewski et al., 2004)

NOT Enhanced by

Statement

Reference

bai¹ has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, non-enhanceable by eca¹

(Bartoszewski et al., 2004)

bai¹ has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, non-enhanceable by put[+]/put¹³⁵

(Bartoszewski et al., 2004)

NOT suppressed by

Statement

Reference

bai¹ has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, non-suppressible by eca¹

(Bartoszewski et al., 2004)

NOT Enhancer of

Statement

Reference

bai¹ is a non-enhancer of embryonic/first instar larval cuticle | dorsal | germline clone phenotype of eca¹

(Bartoszewski et al., 2004)

Suppressor of

Statement

Reference

bai¹ is a suppressor of embryonic/first instar larval cuticle | ventral phenotype of dl¹⁵

(Bartoszewski et al., 2004)

NOT Suppressor of

Statement

Reference

bai¹ is a non-suppressor of embryonic/first instar larval cuticle | dorsal | germline clone phenotype of eca¹

(Bartoszewski et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous dl¹⁵, bai¹ germ-line clones produce embryos that form cuticles lateralised at the filzkorper level - a long dorsalised cuticle with a characteristic filzkorper ring. Embryos lacking maternal bai (bai¹) and also lacking zygotic tkv (tkv^SL144) are completely ventralised. Embryos derived from germ-line clones that are homozygous for bai¹ and heterozygous for put¹³⁵ are weakly ventralised.

(Bartoszewski et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

bai¹ is rescued by bai^UASp.cSa/Scer\GAL4^Act5C.PI

(Saleem et al., 2012)

Not rescued by

bai¹ is not rescued by Scer\GAL4^CY2/bai^UASp.cSa

(Saleem et al., 2012)

bai¹ is not rescued by Scer\GAL4^CY2/bai^UAS.cBa

(Bartoszewski et al., 2004)

bai¹ is not rescued by Scer\GAL4^{VP16.nanos.UTR}/bai^UAS.cBa

(Bartoszewski et al., 2004)

bai¹ is not rescued by bai^+t7.7

(Bartoszewski et al., 2004)

Comments

Expression of bai^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^CY2 fails to rescue the lethality associated with bai¹ mutants.

Expression of bai^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^Act5C.PI rescues the lethality and egg laying defects associated with bai¹ mutants.

(Saleem et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

bai

(Saleem et al., 2012)

bai¹

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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