The severity of the mushroom body α axon misguidance phenotype seen in flies expressing two copies of Wnt5Scer\UAS.cFa under the control of Scer\GAL4c739 is significantly enhanced (to over 10%) if they are also carrying either drl2/+ or Drl-2E124/+. Flies simultaneously carrying both drl2/+ and Drl-2E124/+ and expressing two copies of Wnt5Scer\UAS.cFa under the control of Scer\GAL4c739 show misguidance of α axons in over 40% of cases.
Co-expression of drlScer\UAS.cYa, but not of drlΔintra.Scer\UAS.T:Hsap\MYC, suppresses the distortion of the antennal lobe caused by expression of Wnt5Scer\UAS.cFa under the control of Scer\GAL4GH146.
Expression of Wnt5Scer\UAS.cFa under the control of any of Scer\GAL4how-24B, Scer\GAL4Mef2.PR or Scer\GAL4sr-md710 rescues the lateral transverse muscle attachment defects seen in Wnt5400 mutant stage 16 embryos. Expressing Wnt5Scer\UAS.cFa under the control of Scer\GAL4tey-5053A is unable to rescue the phenotype.
Expression of Wnt5Scer\UAS.cFa under the control of either Scer\GAL4SG18.1 or Scer\GAL4OK72 partially rescues the antennal lobe defects seen in Wnt5400 flies: the abnormal shape is rescued, the abnormal position of the Or47b glomerulus relative to the dorsal edge of the lobe is partially rescued and a commissure connects the antennal lobes in 55% or 77% of cases respectively.