FlyBase Allele Report: Scer\GAL4[NP5286]

General Information

Symbol

Scer\GAL4^NP5286

Species

S. cerevisiae

Name

FlyBase ID

FBal0159796

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}CG17834^NP5286

Carried in Construct

Also Known As

NP5286

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}CG17834^NP5286

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:8,529,866..8,530,517

(Sun et al., 2020, Flybrain Neuron Database et al., 2018.6.28, Tong et al., 2016, Yagi et al., 2016, Perisse et al., 2013, Huang et al., 2012, Pauls et al., 2010, Pauls et al., 2010, Tanaka et al., 2008, Tanaka et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

inner layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

outer layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

core layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

adult stage

adult mushroom body alpha-lobe | restricted

Comment: expression localized to surface of mushroom body alpha-lobe

(Tanaka et al., 2004)

adult antennal lobe projection neuron | subset

(Tanaka et al., 2004)

antennal lobe glomerulus DM1

(Tanaka et al., 2004)

antennal lobe glomerulus VA4

(Tanaka et al., 2004)

antennal lobe glomerulus VC1

(Tanaka et al., 2004)

adult mushroom body beta-lobe | restricted

Comment: expression at surface of beta-lobe

(Tanaka et al., 2004)

adult mushroom body dorsal accessory calyx

(Tanaka et al., 2008)

mushroom body beta lobe posterior layer

(Tanaka et al., 2008)

mushroom body alpha lobe posterior layer

(Tanaka et al., 2008)

mushroom body beta lobe surface layer

(Tanaka et al., 2008)

mushroom body alpha lobe surface layer

(Tanaka et al., 2008)

calyx of adult mushroom body

(Tanaka et al., 2008)

adult mushroom body | restricted

(Tanaka et al., 2004)

alpha/beta Kenyon cell

(Pauls et al., 2010)

alpha/beta surface Kenyon cell | intense

(Perisse et al., 2013)

alpha/beta posterior Kenyon cell | faint

(Perisse et al., 2013)

alpha/beta surface Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28, Yagi et al., 2016)

alpha/beta posterior Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

adult mushroom body alpha-lobe

(Pauls et al., 2010)

adult mushroom body beta-lobe

(Pauls et al., 2010)

Additional Information

Statement

Reference

Expression is observed in ~500 projection neurons in the surface layer of the α and β lobes of the mushroom body.

(Tanaka et al., 2004)

Expression is observed in 2-4 projection neurons in antennal glomeruli DM1, VA4 and VC1.

(Tanaka et al., 2004)

Marker for

adult antennal lobe projection neuron DM1 lPN

(Tanaka et al., 2004)

adult antennal lobe projection neuron VA4 lPN

(Tanaka et al., 2004)

adult antennal lobe projection neuron VC1 lPN

(Tanaka et al., 2004)

alpha/beta Kenyon cell

(Tanaka et al., 2004)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Perisse et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Blocking synaptic transmission (using shi^1.Scer\UAS at restrictive temperatures) from Scer\GAL4^NP5286 neurons significantly impairs sucrose-reinforced appetitive or electric-shock-reinforced aversive memory performance (3 hours after conditioning) compared to controls. Flies undergo conditioning at a permissive temperature (23[o]C), whereas testing is at a restrictive temperature (33[o]C); there are no significant memory differences when training and testing is at permissive temperatures. Scer\GAL4^NP5286>shi^1.Scer\UAS flies exhibit normal olfactory acuity at the restrictive temperature.

(Perisse et al., 2013)

Blocking synaptic transmission in Scer\GAL4^NP5286-expressing neurons for the 12 hour interval 6 hours after spaced training has no effect on aversive long term memory.

Blocking synaptic transmission in Scer\GAL4^NP5286-expressing neurons during the post-training period has no effect on appetitive long term memory (association of an odor with sucrose).

(Huang et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

113663

w^*; P{GawB}CG17834^NP5286 / CyO, P{UAS-lacZ.UW14}UW14

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^NP5286

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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