FlyBase Allele Report: Dmel\Rab8[B229]

General Information

Symbol

Dmel\Rab8^B229

Species

D. melanogaster

Name

FlyBase ID

FBal0159939

Feature type

allele

Associated gene

Dmel\Rab8

Associated Insertion(s)

PBac{5HPw⁺}Rab8^B229

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{5HPw⁺}Rab8^B229

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:19,869,602..19,869,602 [-]

(West et al., 2015, Cooper et al., 2010, Bloomington Drosophila Stock Center, 2004.11.7)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with Df(3L)ED228)

(West et al., 2015)

abnormal neuroanatomy | third instar larval stage (with Rab8¹)

(West et al., 2015)

abnormal neuroanatomy | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Act5C.PI

(West et al., 2015)

abnormal neuroanatomy | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

(West et al., 2015)

abnormal neuroanatomy | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

(West et al., 2015)

abnormal neuroanatomy | third instar larval stage (with Rab8²)

(West et al., 2015)

abnormal neuroanatomy | third instar larval stage (with Rab8³)

(West et al., 2015)

abnormal neuroanatomy | third instar larval stage (with Rab8^Z3007)

(West et al., 2015)

abnormal neurophysiology | third instar larval stage (with Rab8¹)

(West et al., 2015)

lethal - all die during immature adult stage (with Rab8¹)

(West et al., 2015)

Phenotype Manifest In

embryonic/larval motor neuron | third instar larval stage (with Rab8¹)

(West et al., 2015)

embryonic/larval neuromuscular junction | third instar larval stage (with Df(3L)ED228)

(West et al., 2015)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8¹)

(West et al., 2015)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8²)

(West et al., 2015)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8³)

(West et al., 2015)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8^Z3007)

(West et al., 2015)

larval ventral nerve cord | third instar larval stage (with Rab8¹)

(West et al., 2015)

muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8¹)

(West et al., 2015)

muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Act5C.PI

(West et al., 2015)

muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

(West et al., 2015)

muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

(West et al., 2015)

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage (with Rab8¹)

(West et al., 2015)

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

(West et al., 2015)

NMJ bouton | third instar larval stage (with Df(3L)ED228)

(West et al., 2015)

NMJ bouton | third instar larval stage (with Rab8¹)

(West et al., 2015)

NMJ bouton | third instar larval stage (with Rab8²)

(West et al., 2015)

NMJ bouton | third instar larval stage (with Rab8³)

(West et al., 2015)

NMJ bouton | third instar larval stage (with Rab8^Z3007)

(West et al., 2015)

recycling endosome (with Rab8¹)

(West et al., 2015)

Detailed Description

Statement

Reference

Rab8¹/Rab8^B229 mutants show pharate lethality.

All the tested trans-heterozygous allelic combinations of Rab8^B229 mutants display synaptic overgrowth, characterized by significant increase in synaptic bouton number of up to 100% relative to wild-type.

In addition to elevated bouton number, Rab8¹/Rab8^B229 mutants also have a significant increase in neuromuscular junction length, branching, and satellite bouton number, with reduction in synaptic bouton size.

Postsynaptic expression of Rab8^Scer\UAS.cWa under the control of Scer\GAL4^Mhc.PW rescues the increased synaptic bouton number phenotype at muscle 6/7, but not synapse length at neuromuscular junctions in Rab8¹/Rab8^B229 mutants.

Synaptic bouton number or length in muscle 4 is not rescued by muscle expression of Rab8^Scer\UAS.cWa.

Rab8¹/Rab8^B229 mutants display a clear reduction in both fast and slow recycling endosomes at both the NMJ and in motor neuron cell bodies in the larval ventral nerve cord. Upon ultrastructural analysis of mutant synapses, accumulation of large endosome-like, intermediary structures within synaptic boutons are observed. The observed structures, however, are dissimilar and unlikely synaptic vesicles as they are not released, because there is no increase in quantal size during electrophysiological analysis. On the contrary, Rab8¹/Rab8^B229 mutants show decreased quantal size compared with wild-type. These structures are similar to autophagosomal intermediates and they are never observed in wild-type animals.

(West et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by tkv⁷/tkv[+]

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by tkv^k16713/tkv[+]

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by tkv^k16713/tkv⁷

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/kay^Fbz.UAS

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^nSyb.PS/kay^Fbz.UAS

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by POSH^EP1206

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by POSH^EP2248

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Tak1[+]/Tak1¹⁷⁹

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Tak1^RNAi.UAS.cUa/Scer\GAL4^nSyb.PS

(West et al., 2015)

Rab8¹/Rab8^B229 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Tak1^K46R.M.UAS/Scer\GAL4^nSyb.PS

(West et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Hrs^UAS.cUa/Scer\GAL4^nSyb.PS

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by tkv⁷/tkv[+]

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by POSH^EP1206

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by POSH^EP1206

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by POSH^EP2248

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by POSH^EP2248

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Tak1[+]/Tak1¹⁷⁹

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by Tak1[+]/Tak1¹⁷⁹

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Tak1^RNAi.UAS.cUa/Scer\GAL4^nSyb.PS

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by Tak1^RNAi.UAS.cUa/Scer\GAL4^nSyb.PS

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Tak1^K46R.M.UAS/Scer\GAL4^nSyb.PS

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by Tak1^K46R.M.UAS/Scer\GAL4^nSyb.PS

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by tkv^k16713/tkv[+]

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by Hrs^UAS.cUa/Scer\GAL4^nSyb.PS

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by tkv^k16713/tkv[+]

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by tkv^k16713/tkv⁷

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by tkv^k16713/tkv⁷

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/kay^Fbz.UAS

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/kay^Fbz.UAS

(West et al., 2015)

Rab8¹/Rab8^B229 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Scer\GAL4^nSyb.PS/kay^Fbz.UAS

(West et al., 2015)

Rab8¹/Rab8^B229 has NMJ bouton | third instar larval stage phenotype, suppressible by Scer\GAL4^nSyb.PS/kay^Fbz.UAS

(West et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

When present in the genetic background, heterozygous tkv⁷ reduces the synaptic overgrowth in Rab8¹/Rab8^B229 mutants.

When present in the genetic background, heterozygous tkv^k16713 reduces the synaptic overgrowth in Rab8¹/Rab8^B229 mutants.

When present in the genetic background, trans-heterozygous tkv⁷/tkv^k16713 reduces the synaptic overgrowth in Rab8¹/Rab8^B229 mutants.

Neuronal expression of kay^Fbz.Scer\UAS under the control of Scer\GAL4^RapGAP1-OK6 is sufficient to completely alleviate the synaptic overgrowth in Rab8¹/Rab8^B229 mutants.

Neuronal expression of kay^Fbz.Scer\UAS under the control of Scer\GAL4^nSyb.PS is sufficient to completely alleviate the synaptic overgrowth in Rab8¹/Rab8^B229 mutants.

Introducing POSH^EP1206 into a Rab8¹/Rab8^B229 mutant background alleviates the synaptic overgrowth phenotype.

Introducing POSH^EP2248 into a Rab8¹/Rab8^B229 mutant background alleviates the synaptic overgrowth phenotype.

(West et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Rab8¹/Rab8^B229 is partially rescued by Scer\GAL4^Act5C.PI/Rab8^UAS.cWa

(West et al., 2015)

Rab8¹/Rab8^B229 is partially rescued by Scer\GAL4^nSyb.PS/Rab8^UAS.cWa

(West et al., 2015)

Rab8¹/Rab8^B229 is partially rescued by Scer\GAL4^Mhc.PW/Rab8^UAS.cWa

(West et al., 2015)

Comments

Expression of Rab8^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI and the presynaptic driver Scer\GAL4^nSyb.PS is sufficient to completely rescue the increased bouton number at muscles 6/7 Rab8¹/Rab8¹ mutants.

Synaptic bouton number or length in muscle 4 is not rescued by muscle expression of Rab8^Scer\UAS.cWa.

(West et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

16174

y¹ w¹¹¹⁸; PBac{5HPw⁺}Rab8^B229/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab8^B229

(Chang et al., 2024)

Rab8^pBacB229

(Gore et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (5)

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