Allele Dmel\Nf1^c00617

General Information
Symbol	Dmel\Nf1^c00617	Species	D. melanogaster
Name		FlyBase ID	FBal0160134
Feature type	allele	Associated gene	Dmel\Nf1

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	piggyBac transposase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	piggyBac transposase (Bloomington Drosophila Stock Center, 2004.11.6)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:21,811,820..21,811,820 (Bloomington Drosophila Stock Center, 2004.11.6, Buchanan and Davis, 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	PBac{PB}Nf1^c00617 (Bloomington Drosophila Stock Center, 2004.11.6, Buchanan and Davis, 2010)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Bloomington Drosophila Stock Center, 2004.11.6) memory defective (Buchanan and Davis, 2010) small body \| male limited (Buchanan and Davis, 2010) viable (Bloomington Drosophila Stock Center, 2004.11.6)
Phenotype Manifest In

Detailed Description
	Statement Reference Nf1[c00617] males are smaller than control males, while females show no change. Nf1[c00617] flies exhibit 3 minute, 3 hour, and 24 hour memory deficits. In training trial assays, where flies are repeatedly trained with odor avoidance assays, Nf1[P1] flies perform poorly even after one trial, but then exhibit wild-type levels of olfactory memory acquisition. When Nf1[c00617] mutants and controls are both trained with three trials, immediate memory is identical (when performance is normalised to the control so memory decay can be compared). Likewise, memory tested at two subsequent time points is indistinguishable. (Buchanan and Davis, 2010)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Nf1^c00617 is rescued by Nf1^Scer\UAS.cBa/Scer\GAL4^c739 (Buchanan and Davis, 2010) Nf1^c00617 is rescued by Nf1^Scer\UAS.cBa/Scer\GAL4^{Mef2.247.Switch} (Buchanan and Davis, 2010) Nf1^c00617 is rescued by Scer\GAL4^c747/Nf1^Scer\UAS.cBa (Buchanan and Davis, 2010) Nf1^c00617 is rescued by Scer\GAL4^elav-C155/Nf1^Scer\UAS.cBa (Buchanan and Davis, 2010) Nf1^c00617 is rescued by Scer\GAL4^Mz1081/Nf1^Scer\UAS.cBa (Buchanan and Davis, 2010) Nf1^c00617 is rescued by Scer\GAL4^NP0009/Nf1^Scer\UAS.cBa (Buchanan and Davis, 2010)
Not rescued by	Nf1^c00617 is not rescued by Nf1^Scer\UAS.cBa/Scer\GAL4^17d (Buchanan and Davis, 2010) Nf1^c00617 is not rescued by Nf1^Scer\UAS.cBa/Scer\GAL4^c305a (Buchanan and Davis, 2010) Nf1^c00617 is not rescued by Nf1^Scer\UAS.cBa/Scer\GAL4^NP1131 (Buchanan and Davis, 2010) Nf1^c00617 is not rescued by Scer\GAL80^Mef2.PT/Scer\GAL4^c747/Nf1^Scer\UAS.cBa (Buchanan and Davis, 2010)
Comments	Expression of Nf1[Scer\UAS.cBa] in mushroom body neurons during development and adulthood under the control of inducible Scer\GAL4[Mef2.247.Switch] (in a RU486 environment) completely rescues the 3 hour memory phenotype seen in Nf1[c00617] mutants. Expression only during adulthood is sufficient for complete rescue. Expression of Nf1[Scer\UAS.cBa] in α'/β' neurons under the control of Scer\GAL4[c305a] is not sufficient to rescue the 3 hour memory phenotype of Nf1[c00617] homozygous mutants. Expression of Nf1[Scer\UAS.cBa] in γ neurons under the control of Scer\GAL4[NP1131] is not sufficient to rescue the 3 hour memory phenotype of Nf1[c00617] homozygous mutants. Expression of Nf1[Scer\UAS.cBa] throughout the central brain under the control of Scer\GAL4[Mz1081] is sufficient to rescue the 3 hour memory phenotype found in Nf1[c00617] mutants. Expression of Nf1[Scer\UAS.cBa] throughout the central brain under the control of Scer\GAL4[NP0009] is sufficient to rescue the 3 hour memory phenotype found in Nf1[c00617] mutants. Expression of Nf1[Scer\UAS.cBa] in the α/β mushroom body neurons under the control of Scer\GAL4[c739] is sufficient to rescue the 3 hour memory phenotype found in Nf1[c00617] mutants. Expression of Nf1[Scer\UAS.cBa] in the central core of α/β mushroom body neurons under the control of Scer\GAL4[17d] is not sufficient to rescue the 3 hour memory phenotype found in Nf1[c00617] mutants. The Nf1[c00617] 3 hour memory phenotype is fully rescued when Scer\GAL4[c747] is used to express Nf1[Scer\UAS.cBa]. However, flies also carrying Scer\GAL80[Mef2.PT] exhibit a performance score that is indistinguishable from Nf1[c00617] homozygous mutants. (Buchanan and Davis, 2010)
Stocks ( 2 )
Bloomington	10201 w¹¹¹⁸; PBac{PB}Nf1^c00617
Harvard	- PBac{PB}Nf1^c00617
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Nf1^c00617 nf1^c00617 (Buchanan and Davis, 2010)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Buchanan and Davis, 2010, J. Neurosci. 30(30): 10135--10143 A distinct set of Drosophila brain neurons required for neurofibromatosis type 1-dependent learning and memory. [FBrf0211411]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]

Allele Dmel\Nf1c00617

Allele Dmel\Nf1^c00617