Allele Dmel\Lnk^d07478

General Information
Symbol	Dmel\Lnk^d07478	Species	D. melanogaster
Name		FlyBase ID	FBal0160237
Feature type	allele	Associated gene	Dmel\Lnk

Map ( GBrowse )	Untitled Document
Allele class	amorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Slack et al., 2010)
Mutagen	P-element activity (Bloomington Drosophila Stock Center, 2004.11.6)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:21,714,799..21,714,799 (Bloomington Drosophila Stock Center, 2004.11.6, Gene Disruption Project members and Exelixis, 2005, Song et al., 2010, Slack et al., 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion within the first intron. (Slack et al., 2010)
Caused by insertion	P{XP}Lnk^d07478 (Bloomington Drosophila Stock Center, 2004.11.6, Gene Disruption Project members and Exelixis, 2005, Song et al., 2010, Slack et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell number (Song et al., 2010, Slack et al., 2010) decreased cell size (Song et al., 2010, Slack et al., 2010) female semi-sterile \| recessive (Slack et al., 2010) female sterile (Song et al., 2010) fertile (Bloomington Drosophila Stock Center, 2004.11.6) long lived (Song et al., 2010) long lived \| recessive (Slack et al., 2010) male fertile (Song et al., 2010) oxidative stress response defective (Song et al., 2010) small body (with Lnk^Del29) (Slack et al., 2010) small body \| adult stage (Song et al., 2010) small body \| recessive (Slack et al., 2010) small body \| third instar larval stage (Song et al., 2010) starvation stress response defective (Song et al., 2010) starvation stress response defective (with Lnk^f02642) (Song et al., 2010) viable (Bloomington Drosophila Stock Center, 2004.11.6)
Phenotype Manifest In
	egg chamber (Ferguson et al., 2012) wing (Slack et al., 2010) wing (with Lnk^Del29) (Slack et al., 2010) wing blade (Song et al., 2010)
Detailed Description
	Statement Reference Egg chambers arrest in early vitellogenesis in mutant females. (Ferguson et al., 2012) Homozygotes show a 20% reduction in wing area and a 42% reduction in body mass compared to wild-type controls. The mean number of cells per wing and the mean cell size in the wing are both reduced in the mutant flies. A significant increase in both median and maximum lifespan is seen in both homozygous males and females compared to controls. Homozygous females produce significantly fewer eggs compared to wild-type females. Wild-type females mated to homozygous males produce normal numbers of offspring. Lnk[d07478]/Lnk[Del29] animals show a 16% reduction in wing area and a 34% reduction in body mass compared to wild-type controls. (Slack et al., 2010) Body length is decreased by 11% compared to controls in homozygous third instar larvae and body weight is decreased by 21% in homozygous adults. Homozygous adults have wings that are reduced in size due to controls, due to a decrease both in the size and number of cells. Total triglycerides are increased by 92% in the fat bodies of homozygous adults compared to those of control wild-type animals. Hemolymph trehalose levels are increased by 37% in homozygous flies compared to controls. Whole body trehalose and total sugar levels are increased by 102% and 85% respectively in homozygous flies compared to controls. Homozygous and Lnk[d07478]/Lnk[f02642] files show increased survival under starvation conditions compared to wild type. Homozygotes show an increase in lifespan compared to control; the median lifespan is increased by 14% in male and 33% in female flies, while the maximal lifespan is increased by 14% in male and 15% in female flies. Homozygotes show increased resistance to paraquat compared to wild type. (Song et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference Lnk^d07478/Lnk^CR642 is a suppressor of dorsal appendage \| maternal effect phenotype of spn-A^093A/spn-A¹ (Ferguson et al., 2012) Lnk^d07478/Lnk^CR642 is a suppressor of dorsal appendage \| maternal effect phenotype of spn-B^BU (Ferguson et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference The ventralisation phenotype seen in eggs laid by spn-B[BU] females is suppressed if the females are also mutant for Lnk[CR642]/Lnk[d07478]. The ventralisation phenotype seen in eggs laid by spn-A[093A]/spn-A[1] females is suppressed if the females are also mutant for Lnk[CR642]/Lnk[d07478]. (Ferguson et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Lnk^d07478 is rescued by Scer\GAL4^Adh.PF/Lnk^Scer\UAS.cSa (Song et al., 2010, Song et al., 2010)
Comments
Stocks ( 2 )
Bloomington	19274 w¹¹¹⁸; P{XP}Lnk^d07478
Harvard	- P{XP}Lnk^d07478
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dSH2B^D (Song et al., 2010) Lnk^d07478 (Slack et al., 2010) lnk^d07478 (Werz et al., 2009, Ferguson et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Ferguson et al., 2012, J. Cell Sci. 125(6): 1407--1419 Modulation of gurken translation by insulin and TOR signaling in Drosophila. [FBrf0218097] Slack et al., 2010, PLoS Genet. 6(3): e1000881 Regulation of lifespan, metabolism, and stress responses by the Drosophila SH2B protein, Lnk. [FBrf0210418] Song et al., 2010, Cell Metab. 11(5): 427--437 SH2B regulation of growth, metabolism, and longevity in both insects and mammals. [FBrf0210745] Werz et al., 2009, PLoS Genet. 5(8): e1000596 The Drosophila SH2B family adaptor Lnk acts in parallel to chico in the insulin signaling pathway. [FBrf0208673]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]

Allele Dmel\Lnkd07478

Allele Dmel\Lnk^d07478