Open Close
General Information
Symbol
Dmel\Dap160Δ1
Species
D. melanogaster
Name
FlyBase ID
FBal0160635
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{EP} element.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Dap160Q24/Dap160Δ1 third instar larvae have a reduced number of neuroblasts per brain lobe compared to wild-type controls.

Progression through mitosis is delayed in Dap160Q24/Dap160Δ1 larval neuroblasts compared to wild-type neuroblasts; the transit from nuclear envelope breakdown to anaphase onset is 13.37 +/- 4.4 minutes in the mutant neuroblasts compared to 7.76 +/- 2.04 minutes in wild-type neuroblasts.

The levels of membrane uptake (dye internalisation) in Dap160Δ1/Dap160Δ2 mutant synaptic boutons in response to nerve stimulation is significantly reduced compared to controls. Increased uptake is seen when these flies are treated with chlorpromazine.

Total bouton number at the neuromuscular junction is increased in Dap160EMS/Dap160Δ1 larvae compared to controls.

Under high-frequency stimulation conditions (10Hz for 10 minutes), the neuromuscular junctions (NMJs) of Dap160Δ1/Df(2L)bur-K1 larvae show strong synaptic depression to approximately 40% of the initial excitatory junctional potential (EJP) amplitude (in contrast to control NMJs, which are able to maintain EJPs at approximately 90% of the initial amplitude).

During stimulation, Dap160Δ1/Df(2L)bur-K1 boutons are seen to contain cisternae and large membraneous bodies that appear to be contiguous with the plasma membrane.

Dap160Δ1/Df(2L)bur-K1 larvae show reduced dye uptake at the neuromuscular junction compared to controls, indicating a defect in endocytosis.

Dap160EMS/Dap160Δ1 adults become paralysed at temperatures between 34oC and 36oC.

Dap160Δ1/Dap160Δ6 adults show paralysis at 38oC.

Neuromuscular junction (NMJ) morphology is abnormal in Dap160Δ1/Dap160Δ2

third instar larvae; boutons are irregularly spaced and large boutons

are surrounded by smaller satellite boutons. Bouton number normalised

to muscle area is significantly increased in Dap160Δ1/Dap160Δ2

larvae (3.5 +/- 0.07 μm-2) compared to controls (1.7 +/- 0.07 μm-2).

In addition, the boutons show more frequent branching than controls;

the larval NMJ has 26.2 +/- 2.1 branch points per synapse in Dap160Δ1/Dap160Δ2

animals compared to 5.1 +/- 0.3 branch points per synapse for controls.

Neuromuscular junction (NMJ) morphology is abnormal in Dap160Δ1/Dap160Δ3

third instar larvae; boutons are irregularly spaced and large boutons

are surrounded by smaller satellite boutons. Extra branches and boutons

are apparent in the mutant larvae.

In 5mM Ca2+ at 22oC, the amplitude of the excitatory junctional

potential (EJP) at the neuromuscular junction (NMJ) in Dap160Δ1/Dap160Δ2

or Dap160Δ1/Dap160Δ3 third instar larvae is not different

from that of controls. The EJP amplitude at the NMJ of Dap160Δ1/Dap160Δ2

third instar larvae is normal in 5mM Ca2+ at 34oC. The EJP amplitude

at the NMJ of Dap160Δ1/Dap160Δ3 third instar larvae

is normal in 5mM Ca2+ at 36oC. In 0.5 mM Ca2+, the EJP amplitude

at the NMJ of Dap160Δ1/Dap160Δ2 third instar larvae

is normal at 22oC, and is slightly reduced compared to controls at

34oC.

In 0.5mM Ca2+, the frequency of miniature EJPs (mEJPs) at the NMJ

is increased more than 2 fold in Dap160Δ1/Dap160Δ2

larvae compared to controls at 22oC. mEJPs with an unusually large

amplitude are seen at both 22oC and 34oC.

In 0.5mM Ca2+, the frequency of miniature EJPs (mEJPs) at the NMJ

is increased more than 2 fold in Dap160Δ1/Dap160Δ3

larvae compared to controls at 22oC. mEJPs with an unusually large

amplitude are seen at both 22oC and 36oC.

Dap160Δ1/Dap160Δ2 and Dap160Δ1/Dap160Δ3

larvae show a mild defect in endocytosis at 22oC (assayed by dye

uptake in third instar fillet preparations), which is exacerbated at

36oC.

Dap160Δ1/Dap160Δ2 larvae show a stimulation-dependent

decrease in EJP amplitude when stimulated at 10Hz. This decrease is

more severe at 34oC than at 22oC.

Dap160Δ1/Dap160Δ3 larvae show a stimulation-dependent

decrease in EJP amplitude when stimulated at 10Hz. This decrease is

more severe at 36oC than at 22oC.

Ultrastructural analysis of the NMJ shows that the number of vesicles

is reduced to about 60% of controls in Dap160Δ1/Dap160Δ2

larvae at 22oC without stimulation. The number of vesicles is further

reduced to about 23% of controls upon stimulation in 60mM K+ at 34oC.

The number of active zones is not reduced in the mutant larvae compared

to controls, but there is a reduction in the number of vesicles at

the active zones in mutant larvae at 22oC without stimulation and

a further decrease upon stimulation with 60mM K+ at 34oC. There

are an increased number of vesicles with large diameter in the mutant

synapses. Endocytic intermediates, mostly consisting of collared pits

and Ω structures are seen in the mutants (these structures are

rarely seen in controls). The collared pits are seen at both the active

zones and in periactive zones in the mutant larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

Dap160[+]/Dap160Δ1 is a suppressor of decreased body size | pupal stage phenotype of Nf1E2

Phenotype Manifest In
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Dap160Δ1/+ enhances the increase in total bouton number and satellite bouton number at the neuromuscular junction that is seen in nwk1/nwk2 larvae.

The increase in total bouton number at the neuromuscular junction that is seen in Dap160Δ1 larvae is suppressed by witA12/+.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Chromosome probably carries a second lesion, since Dap160Δ1/Dap160Δ2 and Dap160Δ1/Df(2L)bur-K1 animals die later (pharate adult) than Dap160Δ1 homozygotes (third larval instar).

Comments
Comments

Allelic series (based on protein expression data, complementation data and lethal phase): Dap160Δ1 = Dap160Δ2 > Dap160Δ3 = Dap160Δ4 = Dap160Δ5 > Dap160EMS > Dap160Δ6.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (12)