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General Information
Symbol
Dmel\CycAUAS.Tag:HA,Tag:NLS(SV40-largeT)
Species
D. melanogaster
Name
FlyBase ID
FBal0160675
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of CycA which is tagged at the N-terminal end by Tag:HA followed by a nuclear localisation signal (Tag:NLS(SV40-largeT)).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Germline stem cells (GSCs) in newly eclosed males expressing CycAScer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 show a high frequency of centrosome misorientation and frequently undergo mitosis with misoriented spindles (mitosis with misoriented spindles is not seen in wild-type male GSCs).

Adult flies expressing CycAScer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4da.G32 can be recovered. Expression of CycAScer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4hs.2sev results in ectopic S phases.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of CycAScer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4prd.RG1 partially rescues the cell division defects seen in CycAC8LR1 embryos; there is an increase in cell number and a reduction in cell size in the Scer\GAL4prd.RG1 expression domain.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
CycAScer\UAS.T:Ivir\HA1,T:SV40\nls-largeT
CycAScer\UAS.T:Ivir\HA1,T:SV40\nls2
CycAUAS.Tag:HA,Tag:NLS(SV40-largeT)
Name Synonyms
Secondary FlyBase IDs
    References (3)