FB2025_01 , released February 20, 2025
Allele: Dmel\morac02881
Open Close
General Information
Symbol
Dmel\morac02881
Species
D. melanogaster
Name
FlyBase ID
FBal0160739
Feature type
allele
Associated gene
Dmel\mora
Associated Insertion(s)
PBac{PB}morac02881
Carried in Construct
Also Known As
mora1
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
piggyBac activity
(Bloomington Drosophila Stock Center, 2004.11.6)
Progenitor genotype
Associated Insertion(s)
PBac{PB}morac02881
Cytology
Description
Allele components
Component
Use(s)
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      CHORDc02881/+ flies are viable and morphologically normal.

      CHORDc02881/CHORDc02881 flies die as third instar larvae. Mutant larvae are devoid of imaginal discs, and display strongly reduced testes and salivary glands.

      Brain preparations from CHORDc02881 mutant larvae show that in 95% of dividing cells the chromosomes are elongated and irregularly condensed. Mutant cells spend more time in mitosis than wild-type control cells. The majority of cells remain arrested in a prophase-metaphase state. Of those that do escape into anaphase, strong defects in chromosome segregation are observed. 30% of mutant neuroblasts are polyploid, compared to 0.2% in control cells. In addition, an increase in the frequency of apoptotic cells is observed in mutant tissue (7.7%) compared to control brain tissue (0.5%).

      Supernumerary centrosomes are observed in CHORDc02881 mutant brain cells.

      (Ferretti et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      morac02881 has lethal phenotype, non-suppressible by Scer\GAL4Act5C.PI/Hsap\CHORDC1UAS.cFa

      (Ferretti et al., 2010)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\CHORDC1Scer\UAS.cFa under the control of Scer\GAL4Act5C.PI in CHORDc02881 mutant flies rescues the imaginal disc and testis phenotype, and these tissues appear mostly normal. Most mitotic divisions observed in brain tissue are completely normal None of the observed mitotic figures were polyploid, and 98% of dividing cells display two centrosomes. These flies still die during metamorphosis.

      (Ferretti et al., 2010)
      Complementation and Rescue Data
      Not rescued by

      morac02881 is not rescued by moraUAS.cFa

      (Ferretti et al., 2010)
      Comments

      Expression of CHORDScer\UAS.cFa under the control of Scer\GAL4Act5C.PI in CHORDc02881 mutant animals fails to rescue lethality.

      (Ferretti et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      CHORDc02881
      mora1
      (Palumbo et al., 2020, Ferretti et al., 2010)
      morac02881
      Name Synonyms
      Secondary FlyBase IDs
        References (3)