Allele Dmel\CHORD^c02881

General Information
Symbol	Dmel\CHORD^c02881	Species	D. melanogaster
Name		FlyBase ID	FBal0160739
Feature type	allele	Associated gene	Dmel\CHORD

Allele class
Mutagen	piggyBac transposase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	piggyBac transposase (Bloomington Drosophila Stock Center, 2004.11.6)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	PBac{PB}CHORD^c02881 (Bloomington Drosophila Stock Center, 2004.11.6)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| third instar larval stage (Ferretti et al., 2010) mitotic cell cycle defective \| third instar larval stage (Ferretti et al., 2010)
Phenotype Manifest In
	centrosome \| third instar larval stage (Ferretti et al., 2010) condensed chromosome \| third instar larval stage (Ferretti et al., 2010) embryonic/larval salivary gland \| third instar larval stage (Ferretti et al., 2010) imaginal disc \| third instar larval stage (Ferretti et al., 2010) neuroblast \| third instar larval stage (Ferretti et al., 2010) testis \| third instar larval stage (Ferretti et al., 2010)
Detailed Description
	Statement Reference CHORD[c02881]/+ flies are viable and morphologically normal. CHORD[c02881]/CHORD[c02881] flies die as third instar larvae. Mutant larvae are devoid of imaginal discs, and display strongly reduced testes and salivary glands. Brain preparations from CHORD[c02881] mutant larvae show that in 95% of dividing cells the chromosomes are elongated and irregularly condensed. Mutant cells spend more time in mitosis than wild-type control cells. The majority of cells remain arrested in a prophase-metaphase state. Of those that do escape into anaphase, strong defects in chromosome segregation are observed. 30% of mutant neuroblasts are polyploid, compared to 0.2% in control cells. In addition, an increase in the frequency of apoptotic cells is observed in mutant tissue (7.7%) compared to control brain tissue (0.5%). Supernumerary centrosomes are observed in CHORD[c02881] mutant brain cells. (Ferretti et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference CHORD^c02881 has mitotic cell cycle defective phenotype, suppressible by Scer\GAL4^Act5C.PI/Hsap\CHORDC1^Scer\UAS.cFa (Ferretti et al., 2010)
NOT suppressed by
Statement Reference CHORD^c02881 has lethal phenotype, non-suppressible by Scer\GAL4^Act5C.PI/Hsap\CHORDC1^Scer\UAS.cFa (Ferretti et al., 2010)
Phenotype Manifest In
Suppressed by
Statement Reference CHORD^c02881 has centrosome \| third instar larval stage phenotype, suppressible by Scer\GAL4^Act5C.PI/Hsap\CHORDC1^Scer\UAS.cFa (Ferretti et al., 2010) CHORD^c02881 has imaginal disc \| third instar larval stage phenotype, suppressible by Scer\GAL4^Act5C.PI/Hsap\CHORDC1^Scer\UAS.cFa (Ferretti et al., 2010) CHORD^c02881 has testis \| third instar larval stage phenotype, suppressible by Scer\GAL4^Act5C.PI/Hsap\CHORDC1^Scer\UAS.cFa (Ferretti et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\CHORDC1[Scer\UAS.cFa] under the control of Scer\GAL4[Act5C.PI] in CHORD[c02881] mutant flies rescues the imaginal disc and testis phenotype, and these tissues appear mostly normal. Most mitotic divisions observed in brain tissue are completely normal None of the observed mitotic figures were polyploid, and 98% of dividing cells display two centrosomes. These flies still die during metamorphosis. (Ferretti et al., 2010)
Complementation & Rescue Data
Not rescued by	CHORD^c02881 is not rescued by CHORD^Scer\UAS.cFa (Ferretti et al., 2010)
Comments	Expression of CHORD[Scer\UAS.cFa] under the control of Scer\GAL4[Act5C.PI] in CHORD[c02881] mutant animals fails to rescue lethality. (Ferretti et al., 2010)
Stocks ( 2 )
Bloomington	11130 w¹¹¹⁸; PBac{PB}CHORD^c02881/TM6B, Tb¹
Harvard	- PBac{PB}CHORD^c02881
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CHORD^c02881 mora¹ (Ferretti et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Ferretti et al., 2010, Dev. Cell 18(3): 486--495 Morgana/chp-1, a ROCK Inhibitor Involved in Centrosome Duplication and Tumorigenesis. [FBrf0210216]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]

Allele Dmel\CHORDc02881

Allele Dmel\CHORD^c02881