Allele Dmel\Trh^c01440

General Information
Symbol	Dmel\Trh^c01440	Species	D. melanogaster
Name		FlyBase ID	FBal0160850
Feature type	allele	Associated gene	Dmel\Trh
Also Known As	CG9122^c01440

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	piggyBac transposase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	piggyBac transposase (Bloomington Drosophila Stock Center, 2004.11.6)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:1,190,496..1,190,496 (Bloomington Drosophila Stock Center, 2004.11.6, Neckameyer et al., 2007, Neckameyer, 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	PBac{PB}Trh^c01440 (Bloomington Drosophila Stock Center, 2004.11.6, Neckameyer et al., 2007, Neckameyer, 2010)
Cytology
Phenotypic Data
Phenotypic Class
	feeding behavior defective \| adult stage (Neckameyer et al., 2007) feeding behavior defective \| larval stage (Neckameyer et al., 2007) fertile (Bloomington Drosophila Stock Center, 2004.11.6) neuroanatomy defective \| larval stage (Neckameyer, 2010) partially lethal - majority die \| larval stage (Neckameyer et al., 2007) smell perception defective (Neckameyer et al., 2007) viable (Bloomington Drosophila Stock Center, 2004.11.6)
Phenotype Manifest In

Detailed Description
	Statement Reference Trh[c01440] mutants exhibit an increase in neuronal arborization and varicosity number, compared to wild-type. Trh[c01440] mutant second instar larvae that are directly exposed to 10[-6]M 5-HT from 16 hours after egg laying to hatching, exhibit feeding behaviour that is indistinguishable from parental control flies, and a decrease in the complexity of serotonergic gut fibers projecting to the proventriculus. Hypersensitivity to 5-HTP is also rescued. (Neckameyer, 2010) Larval feeding (measured by the number of mouth hook contractions in a yeast solution) is significantly affected by the decrease in neuronal 5-HT in Trh[c01440] mutants. This is unlikely to be due to aberrant motor control because body wall contractions (measured on an agar substrate) are unaffected. Heart rate in Trh[c01440] mutant white prepupae is significantly reduced relative to controls. Trh[c01440] mutants display an unusually strong aversion to the odorant nonanol, while showing a strong attraction to the odorant heptanol (as do controls). Female adult motor behaviour is significantly depressed by the decrease in neuronal 5-HT in Trh[c01440] mutants. Adult feeding behaviour is significantly decreased compared to controls, in both males and females. Embryonic survival of Trh[c01440] is enhanced relative to controls. The number of eclosing animals is decreased in Trh[c01440] mutants compared to controls, suggesting that Trh activity is critical, but no vital, during the larval and/or pupal stages. Adult survival does not require Trh activity for either female or male flies. (Neckameyer et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	10531 w¹¹¹⁸; PBac{PB}Trh^c01440
Harvard	- PBac{PB}Trh^c01440
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CG9122^c01440 (Gene Disruption Project members and Exelixis, 2005, Neckameyer, 2010) Trh^c01440
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Neckameyer, 2010, Dev. Neurosci. 32(3): 217--237 A trophic role for serotonin in the development of a simple feeding circuit. [FBrf0211600] Neckameyer et al., 2007, Genes Brain Behav. 6(8): 756--769 Compartmentalization of neuronal and peripheral serotonin synthesis in Drosophila melanogaster. [FBrf0201679]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]

Allele Dmel\Trhc01440

Allele Dmel\Trh^c01440