FlyBase Allele Report: Dmel\Opa1[EY09863]

General Information

Symbol

Dmel\Opa1^EY09863

Species

D. melanogaster

Name

FlyBase ID

FBal0160917

Feature type

allele

Associated gene

Dmel\Opa1

Associated Insertion(s)

P{EPgy2}Opa1^EY09863

Carried in Construct

Also Known As

opa1^EP, opa1^{p{EPgy2}CG8479}

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}Opa1^EY09863

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:14,235,422..14,235,422 [-]

(Ponte et al., 2020, Park et al., 2009, Yang et al., 2008, McQuibban et al., 2006, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage | somatic clone

(Huang et al., 2015)

lethal - all die before end of first instar larval stage

(McQuibban et al., 2006)

some die during first instar larval stage

(McQuibban et al., 2006)

Phenotype Manifest In

eye | somatic clone

(Huang et al., 2015)

indirect flight muscle cell

(Park et al., 2010)

mitochondrion

(Park et al., 2010)

mitochondrion | germline clone

(McQuibban et al., 2006)

Nebenkern | germline clone

(McQuibban et al., 2006)

retina | somatic clone

(Huang et al., 2015)

Detailed Description

Statement

Reference

ERG recordings from Opa1^EY09863 homozygous mutant clones in the eye show no off-transient.

(Huang et al., 2015)

Heterozygotes show defects in mitochondrial morphology in the indirect flight muscles, with smaller than normal mitochondria being seen between the muscle fibers.

(Park et al., 2010)

No effect on mitochondrial network morphology in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster is seen in opa1-like^EY09863/+ animals.

(Yang et al., 2008)

opa1-like^EY09863 mitotic clones in the male germline (i.e. mutant spermatids) exhibit irregularly-shaped nebenkerns, composed of many individual mitochondria that have coalesced beside the nucleus, but have failed to fuse.

(McQuibban et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

opa1-like[+]/Opa1^EY09863 is a suppressor of visible phenotype of park¹

(Park et al., 2009)

opa1-like[+]/Opa1^EY09863 is a suppressor of visible phenotype of Pink1^B9

(Park et al., 2009, Yang et al., 2008)

NOT Suppressor of

Statement

Reference

Opa1^EY09863 is a non-suppressor of visible phenotype of Scer\GAL4^Tub.PU, park^VDRC.cUa

(Fernandes and Rao, 2011)

opa1-like[+]/Opa1^EY09863 is a non-suppressor of flightless phenotype of Pink1^B9

(Park et al., 2009)

opa1-like[+]/Opa1^EY09863 is a non-suppressor of flightless phenotype of park¹

(Park et al., 2009)

Phenotype Manifest In

Suppressor of

Statement

Reference

opa1-like[+]/Opa1^EY09863 is a suppressor of wing phenotype of park¹

(Park et al., 2009)

opa1-like[+]/Opa1^EY09863 is a suppressor of adult thorax phenotype of park¹

(Park et al., 2009)

opa1-like[+]/Opa1^EY09863 is a suppressor of wing phenotype of Pink1^B9

(Park et al., 2009, Yang et al., 2008)

opa1-like[+]/Opa1^EY09863 is a suppressor of adult thorax phenotype of Pink1^B9

(Park et al., 2009)

opa1-like[+]/Opa1^EY09863 is a suppressor of mitochondrion & dopaminergic neuron phenotype of Pink1^B9

(Park et al., 2009)

opa1-like[+]/Opa1^EY09863 is a suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Yang et al., 2008)

opa1-like[+]/Opa1^EY09863 is a suppressor of mitochondrion & indirect flight muscle phenotype of Pink1^B9

(Yang et al., 2008)

opa1-like[+]/Opa1^EY09863 is a suppressor of mitochondrial inner membrane & indirect flight muscle phenotype of Pink1^B9

(Yang et al., 2008)

opa1-like[+]/Opa1^EY09863 is a suppressor of mitochondrion & dorso-lateral dopaminergic neuron phenotype of Pink1^B9

(Yang et al., 2008)

NOT Suppressor of

Statement

Reference

Opa1[+]/Opa1^EY09863 is a non-suppressor of wing phenotype of Scer\GAL4^Tub.PU, park^VDRC.cUa

(Fernandes and Rao, 2011)

opa1-like[+]/Opa1^EY09863 is a non-suppressor of mitochondrion & dorso-lateral dopaminergic neuron phenotype of Pink1^UAS.cYa, Scer\GAL4^ple.PF

(Yang et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The downturned wing and crushed thorax phenotypes but not the flightless phenotype of Pink1^B9 mutant flies is significantly suppressed by opa1-like^EY09863/+.

The swollen mitochondria phenotype seen in dopaminergic neurons of Pink1^B9 mutants is significantly rescued by opa1-like^EY09863/+.

(Park et al., 2009)

The abnormal wing posture phenotype and the abnormal morphology of the indirect flight muscles and their mitochondria which is seen in Pink1^B9 flies is suppressed by opa1-like^EY09863/+.

The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in Pink1^B9 animals is suppressed by opa1-like^EY09863/+.

The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1^Scer\UAS.cYa under the control of Scer\GAL4^ple.PF is not modified by opa1-like^EY09863/+.

(Yang et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

20054

y¹ w^67c23; P{EPgy2}Opa1^EY09863/CyO

Kyoto

114669

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{EPgy2}Opa1^EY09863 /CyO y⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG8479^EY09863

Opa1^EY09863

(Ponte et al., 2020)

opa1-like^EY09863

(Huang et al., 2015)

opa1^EP

(Banerjee and Chinthapalli, 2014, Park et al., 2010, Park et al., 2009)

opa1^EY09863

(Fernandes and Rao, 2011)

opa1^{p{EPgy2}CG8479}

(Park et al., 2010, Park et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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