Allele Dmel\loqs^f00791

General Information
Symbol	Dmel\loqs^f00791	Species	D. melanogaster
Name		FlyBase ID	FBal0161104
Feature type	allele	Associated gene	Dmel\loqs

Map ( GBrowse )	Untitled Document
Allele class	hypomorphic allele - genetic evidence
Mutagen	piggyBac transposase
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Forstemann et al., 2005, Bilen et al., 2006)
Mutagen	piggyBac transposase (Bloomington Drosophila Stock Center, 2004.11.6)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2L:13,382,714..13,382,714 (Bloomington Drosophila Stock Center, 2004.11.6, Forstemann et al., 2005, Reich et al., 2009, Marques et al., 2010, Liu et al., 2012, Fukunaga et al., 2012)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion 57bp upstream of the loqs transcription start site. (Forstemann et al., 2005) The insertion of a piggybac element within the first exon and 221 nucleotides upstream of the translational start codon of the loqs gene. (Jiang et al., 2005)
Caused by insertion	PBac{WH}loqs^f00791 (Bloomington Drosophila Stock Center, 2004.11.6, Forstemann et al., 2005, Reich et al., 2009, Marques et al., 2010, Liu et al., 2012, Fukunaga et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Jiang et al., 2005, Bennett et al., 2008) female sterile \| recessive (Forstemann et al., 2005) lethal \| embryonic stage \| paternal effect (Forstemann et al., 2005) lethal \| recessive (Bennett et al., 2008) locomotor behavior defective \| adult stage \| recessive (Bennett et al., 2008) male semi-sterile (Jiang et al., 2005) male sterile \| partially (Forstemann et al., 2005, Bennett et al., 2008) neuroanatomy defective \| conditional (Liu et al., 2012) neuroanatomy defective \| embryonic stage (Bennett et al., 2008) short lived (Liu et al., 2012) viable (Forstemann et al., 2005, Liu et al., 2012)
Phenotype Manifest In
	egg chamber (Jiang et al., 2005) eye (Bilen et al., 2006) female germline stem cell (Forstemann et al., 2005) female germline stem cell (with loqs^KO) (Park et al., 2007) follicle cell (Forstemann et al., 2005) germarium (Forstemann et al., 2005, Jiang et al., 2005) lamina \| conditional (Liu et al., 2012) longitudinal connective (Bennett et al., 2008) ovariole (Forstemann et al., 2005) ovary (Forstemann et al., 2005, Jiang et al., 2005) retina \| conditional (Liu et al., 2012) spectrosome (Forstemann et al., 2005) testis (Forstemann et al., 2005) ventral nerve cord commissure (Bennett et al., 2008)
Detailed Description
	Statement Reference loqs[f00791] mutant flies have a significantly shortened lifespan and show late-onset brain morphological deterioration. The brains of young adults appear normal, but by 25 days the flies have developed large vacuoles in the retina and lamina of the brain. (Liu et al., 2012) loqs[f00791] mutants display a reduction in dsRNA processing and a subsequent ~60% reduction in siRNA levels. (Marques et al., 2010) The longitudinal and commissural axon structures of the central nervous system are disrupted in homozygous embryos. Approximately 70% of homozygotes die before adulthood, and surviving males have reduced fertility and surviving females are sterile. Surviving adults are often stuck in the food. Homozygous adults have significantly reduced locomotor activity compared to controls. (Bennett et al., 2008) loqs[f00791]/loqs[KO] females have an average of 0.5 germline stem cells (GSCs) per germarium at 10 days after eclosion, compared to an average of 2-3 GSCs per germarium in wild-type females at this age. Differentiated cystoblasts with posterior-positioned fusomes are often seen at the normal location of GSCs in the germaria of loqs[f00791]/loqs[KO] females. (Park et al., 2007) loqs[f00791] mutants exhibit minimal disruption to the eye. (Bilen et al., 2006) Only 17% of embryos derived from homozygous males mated to wild-type females hatch. Mutant males contain crystals of Ste protein in their testes. Mutant females have small ovaries and are completely sterile, failing to lay any eggs. The mutant ovarioles contain a smaller than normal germarium and contain only 2 or 3 previtellogenic egg chambers and a late-stage egg chamber (in contrast to the developmentally ordered array of 6-8 egg chambers seen in wild-type ovarioles). The mutant germarium contains only a few germ-line cells, which are not organised into distinct cysts. Spectrosomes can not be detected in the mutant germaria of 3-4 day old females, suggesting that no stem cells remain in these flies. The follicle cell layer is significantly reduced compared to wild-type. Mature oocytes in the mutant ovarioles have normal dorsal appendages. (Forstemann et al., 2005) loqs^f00791 mutant females are completely sterile while males are approximately 60-70% sterile when compared with the control crosses between heterozygous and wild-type flies. Although mutant testes appear normal, mutant ovaries contain a few maturing egg chambers and a shriveled germarium with few healthy germline stem cells. The mutant ovary does not sustain continuous egg chamber production since germline stem cells can not be properly maintained. (Jiang et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\ATXN3^{fl.Q84.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791 is an enhancer of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006)
Suppressor of
Statement Reference loqs^f00791/loqs^f00791 is a suppressor \| partially of eye color defective phenotype of w^IR.GMR (Forstemann et al., 2005, Forstemann et al., 2005)
Phenotype Manifest In
Enhancer of
Statement Reference loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN3^{fl.Q84.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN3^{fl.Q84.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) loqs^f00791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006)
Suppressor of
Statement Reference loqs[+]/loqs^f00791 is a suppressor of male germline cell phenotype of mael^M391/Df(3L)79E-F (Pek et al., 2009) loqs^f00791/loqs^f00791 is a suppressor \| partially of pigment cell phenotype of w^IR.GMR (Forstemann et al., 2005, Forstemann et al., 2005) loqs^f00791/r2d2¹ is a suppressor \| partially of eye phenotype of w^IR.GMR (Marques et al., 2010) loqs^f00791 is a suppressor \| partially of eye phenotype of w^IR.GMR (Marques et al., 2010)
NOT Suppressor of
Statement Reference loqs^f00791/loqs^KO is a non-suppressor of female germline cell phenotype of bam^unspecified (Park et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference loqs[f00791] mutant flies carrying two copies of w[IR.GMR] exhibit impaired w silencing and display orange-red eyes. loqs[f00791] r2d2[1] double mutant flies carrying two copies of w[IR.GMR] exhibit impaired w silencing and display orange-red eyes. loqs[f00791] r2d2[1] double mutants display a reduction in dsRNA processing and a subsequent ~80% reduction in siRNA levels. (Marques et al., 2010) The differentiation defects seen in the testes of mael[M391]/Df(3L)79E-F males are partially rescued by loqs[f00791]/+. (Pek et al., 2009) The germ cell tumour phenotype seen in bam[unspecified] females is not suppressed by loqs[f00791]/loqs[KO]. The double mutant germaria retain germline stem cells. (Park et al., 2007) Eye degeneration due to expression of Hsap\MJD[tr.Q78.Scer\UAS.T:Ivir\HA1] in the eye (under the control of Scer\GAL4[GMR.PF]) is enhanced in a loqs[f00791] background, resulting in a severely degenerated eye with complete loss of pigmentation. Eye degeneration due to expression of Hsap\MJD[fl.Q84.Scer\UAS.T:Hsap\MYC] in the eye (under the control of Scer\GAL4[GMR.PF]) is enhanced in a loqs[f00791] background, resulting in dramatically reduced retinal thickness. Eye degeneration due to expression of Hsap\MAPT[Scer\UAS.cWa] in the eye (under the control of Scer\GAL4[GMR.PF]) is enhanced in a loqs[f00791] background. Eye degeneration due to expression of Hsap\MAPT[R406W.Scer\UAS] in the eye (under the control of Scer\GAL4[GMR.PF]) is enhanced in a loqs[f00791] background. (Bilen et al., 2006) The silencing of the w gene seen in flies carrying P{GMR-wIR} is less efficient if the flies are also homozygous for loqs^f00791; flies carrying two copies of P{GMR-wIR} and homozygous for loqs^f00791 do not have completely white eyes (in contrast to flies carrying two copies of P{GMR-wIR} in a wild-type background). (Forstemann et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	loqs^f00791/loqs^KO is rescued by loqs^{PB.T:Hsap\MYC} (Park et al., 2007) loqs^f00791/loqs^KO is rescued by Scer\GAL4^{nos.UTR.T:Hsim\VP16}/loqs^{PB.Scer\UAS.P\T.T:Hsap\MYC} (Park et al., 2007)
Not rescued by	loqs^f00791/loqs^KO is not rescued by loqs^{PA.T:Hsap\MYC} (Park et al., 2007) loqs^f00791/loqs^KO is not rescued by loqs^{PB.Scer\UAS.P\T.T:Hsap\MYC}/Scer\GAL4^bab1-Agal4-5 (Park et al., 2007) loqs^f00791/loqs^KO is not rescued by Scer\GAL4^C587/loqs^{PB.Scer\UAS.P\T.T:Hsap\MYC} (Park et al., 2007)
Comments
Stocks ( 3 )
Bloomington	18371 w¹¹¹⁸; PBac{WH}loqs^f00791/CyO
Harvard	- PBac{WH}loqs^f00791
Kyoto	114651 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; PBac{WH}loqs^f00791 P{neoFRT}40A/CyO y⁺
Notes on Origin
Discoverer
	Separable from: a second mutation on the chromosome. The loqs[f00791] mutation does not cosegregate with the female sterility of the "f00791" chromosome. (Park et al., 2007)
Comments
	Precise excision of the insertion reverts the female sterile phenotype. (Forstemann et al., 2005)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG6866^f00791 (Gene Disruption Project members and Exelixis, 2005, ) dRax^f00791 (Bennett et al., 2008) loqs⁰⁰⁷⁹¹ (Vagin et al., 2006) loqs^f00791 (Czech et al., 2008, Park et al., 2007, Liu et al., 2011, Dufourt et al., 2011, Park et al., 2007, Yang et al., 2007, Zhou et al., 2009, Reich et al., 2009, Pek et al., 2009, Marques et al., 2010, Liu et al., 2012, Fukunaga et al., 2012) R3D1^f00791 (Bilen et al., 2006) r3d1^PB (Jiang et al., 2005)
Name Synonym
Secondary FlyBase IDs

References ( 21 )

Generate a list of	All references relating to this allele ( 21 )

List References by type	Research paper ( 16 ) Supplementary material ( 2 ) Personal communication to FlyBase ( 2 ) Abstract ( 1 )
Recent research papers ( 4 )
	Fukunaga et al., 2012, Cell 151(3): 533--546 Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals. [FBrf0219804] Liu et al., 2012, Nature 482(7386): 519--523 The microRNA miR-34 modulates ageing and neurodegeneration in Drosophila. [FBrf0217556] Dufourt et al., 2011, DNA Res. 18(6): 451--461 Polycomb group-dependent, heterochromatin protein 1-independent, chromatin structures silence retrotransposons in somatic tissues outside ovaries. [FBrf0216699] Liu et al., 2011, Curr. Biol. 21(22): 1888--1893 The Exoribonuclease Nibbler Controls 3' End Processing of MicroRNAs in Drosophila. [FBrf0216782] Show all research papers ...

Allele Dmel\loqsf00791

Allele Dmel\loqs^f00791