FlyBase Allele Report: Dmel\serp[e02821]

General Information

Symbol

Dmel\serp^e02821

Species

D. melanogaster

Name

FlyBase ID

FBal0161685

Feature type

allele

Associated gene

Dmel\serp

Associated Insertion(s)

PBac{RB}serp^e02821

Carried in Construct

Also Known As

serp^RB

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

piggyBac activity

Nature of the Allele

Allele class

loss of function allele

(Luschnig et al., 2006)

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{RB}serp^e02821

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{RB}

gene trap

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:19,704,783..19,704,783 [+]

(Förster and Luschnig, 2012, Cooper et al., 2010, Luschnig et al., 2006, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cuticle

(Luschnig et al., 2006)

embryonic/first instar larval cuticle

(Luschnig et al., 2006)

embryonic/larval tracheal dorsal trunk

(Luschnig et al., 2006)

embryonic/larval tracheal section

(Luschnig et al., 2006)

larval tracheal system

(Dong et al., 2014)

larval tracheal system, with serp^GD6046

(Dong et al., 2014)

Detailed Description

Statement

Reference

Homozygous stage 16 embryos have over-elongated tracheal tubes, while heterozygotes have normal tracheal tube morphology.

Expression of serp^GD6046 under the control of Scer\GAL4^ppl.PP in a serp^e02821/+ background results in abnormally elongated tracheal tubes.

(Dong et al., 2014)

Homozygous serp^e02821 mutants do not exhibit any defects early in tracheal development. Dorsal trunk branches budded, fused, and dilated normally. However, during stage 15, the dorsal trunk begins to elongate inappropriately and becomes convoluted.

serp^e02821 mutants show expanded and deformed cuticles, associated with a lack of cuticle rigidity.

(Luschnig et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

serp^e02821 has embryonic/first instar larval cuticle phenotype, enhanceable by verm^KG07819

(Luschnig et al., 2006)

serp^e02821 has cuticle phenotype, enhanceable by verm^KG07819

(Luschnig et al., 2006)

serp^e02821/Df(3L)Exel6135 has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by verm^KG07819

(Luschnig et al., 2006)

serp^e02821/Df(3L)Exel6135 has embryonic/larval tracheal section phenotype, enhanceable by verm^KG07819

(Luschnig et al., 2006)

serp^e02821/Df(3L)Exel6135 has embryonic/first instar larval cuticle phenotype, enhanceable by verm^KG07819

(Luschnig et al., 2006)

serp^e02821/Df(3L)Exel6135 has cuticle phenotype, enhanceable by verm^KG07819

(Luschnig et al., 2006)

serp^e02821 has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by verm^KG07819

(Luschnig et al., 2006)

serp^e02821 has embryonic/larval tracheal section phenotype, enhanceable by verm^KG07819

(Luschnig et al., 2006)

Enhancer of

Statement

Reference

serp^e02821 is an enhancer of embryonic/larval tracheal dorsal trunk phenotype of verm^KG07819/Df(3L)Exel6135

(Luschnig et al., 2006)

serp^e02821 is an enhancer of embryonic/larval tracheal section phenotype of verm^KG07819/Df(3L)Exel6135

(Luschnig et al., 2006)

serp^e02821 is an enhancer of embryonic/first instar larval cuticle phenotype of verm^KG07819/Df(3L)Exel6135

(Luschnig et al., 2006)

serp^e02821 is an enhancer of cuticle phenotype of verm^KG07819/Df(3L)Exel6135

(Luschnig et al., 2006)

Other

Statement

Reference

serp^e02821, verm^KG07819 has embryonic/larval tracheal section phenotype

(Förster and Luschnig, 2012)

serp^e02821, verm^KG07819 has embryonic/larval cuticle phenotype

(Gangishetti et al., 2012)

serp^e02821, verm^KG07819 has dorsal tracheal branch primordium phenotype

(Luschnig et al., 2006)

serp^e02821, verm^KG07819 has tracheal transverse connective primordium phenotype

(Luschnig et al., 2006)

verm^KG07819/Df(3L)Exel6135, serp^e02821 has dorsal tracheal branch primordium phenotype

(Luschnig et al., 2006)

verm^KG07819/Df(3L)Exel6135, serp^e02821 has tracheal transverse connective primordium phenotype

(Luschnig et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Stage 16 serp^e02821 verm^KG07819 double mutant embryos show excessively elongated tracheae with long apical cell profiles.

(Förster and Luschnig, 2012)

Loss of chitin orientation is seen in the cuticle of serp^e02821 verm^KG07819 larvae, although layering of the cuticle is not affected.

(Gangishetti et al., 2012)

serp^e02821 LCBP1^KG07819 double mutants display an enhanced tracheal phenotype. The dorsal trunk begins to elongate excessively at stage 15 and by stage 16 is approximately 40% longer than normal and highly convoluted. Similar effects are observed in other branches including the transverse connective, although the effects are not as pronounced in smaller-caliber branches, such as the dorsal branch. While the apical (inner) cell surfaces are elongated in serp^e02821 LCBP1^KG07819 mutants the basal (outer) side of tracheal cells does not appear to be similarly elongated and does not follow the tortuous path of the lumen. The tracheal pericellular-diffusion barrier is not compromised in serp^e02821 LCBP1^KG07819 mutants.

serp^e02821 LCBP1^KG07819 double mutants exhibit morphological defects in chitin structure, apparent by stage 14, several hours before the elongated-tube phenotype begins to manifest. In contrast to wild-type, in serp^e02821 LCBP1^KG07819 trachea the fibrous structure of the chitin cylinder is abolished and the surface of the cylinder is irregular. Also, the small gap between the chitin cylinder and the apical surface is absent in serp^e02821 LCBP1^KG07819 mutants. This double mutant also affects embryo body shape, presumably through altering the structure and rigidity of epidermal cuticle.

The phenotype of hemizygous serp^e02821 LCBP1^KG07819 embryos (in trans to Df(3L)Exel6135) is indistinguishable from homozygous serp^e02821 LCBP1^KG07819 embryos. In contrast to the dramatic effects of the mutations on tracheal length and shape, there is little or no effect of the mutations on the diameter of the tubes. The dorsal trunk exhibits its characteristic posterior to anterior taper and is of normal caliber except for slight constrictions that are occasionally observed near dorsal trunk fusion joints.

(Luschnig et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

serp^76BDk-3

(Cook, 2008.12.1)

Rescued by

serp^e02821 is rescued by Scer\GAL4^btl.PU/serp^UAS.cLa.EGFP

(Dong et al., 2014)

serp^e02821 is rescued by Scer\GAL4^ppl.PP/serp^UAS.cLa.EGFP

(Dong et al., 2014)

serp^e02821 is rescued by Scer\GAL4^twi.PU/serp^UAS.cLa.EGFP

(Dong et al., 2014)

Comments

Expression of serp^{Scer\UAS.cLa.T:Avic\GFP-EGFP} under the control of either Scer\GAL4^btl.PU, Scer\GAL4^ppl.PP or Scer\GAL4^twi.PU rescues the over-elongated tracheal tube phenotype seen in homozygous serp^e02821 embryos.

(Dong et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG32209^e02821

serp^RB

(Göpfert et al., 2025, Dong et al., 2014, Förster and Luschnig, 2012, Gangishetti et al., 2012, Luschnig et al., 2006)

serp^e02821

(Cook, 2008.12.1)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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