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General Information
Symbol
Dmel\DopEcRc02142
Species
D. melanogaster
Name
FlyBase ID
FBal0161995
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DopEcRPB1
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion in the second intron.
Insertion components
PBac{PB}DopEcRc02142
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
DopEcRc02142 homozygous adults show an increased resistance to ethanol as compared to controls: they show an increased time until sedation, despite an apparently normal tolerance development following daily exposure; they also show suppression of a normal ethanol-induced disinhibition, as shown by the virtual lack of inter-male courtship upon continual exposure.
DopEcRc02142 homozygous and (to a lesser extent) heterozygous as well as DopEcRc02142/DopEcRGAL4 transheterozygous adults show resistance to ethanol-induced sedation: it takes significantly longer for them cease all movement upon ethanol exposure, although they begin to lose posture around the same time as controls. The ethanol absorption and metabolism of the mutants is normal. However, significantly fewer DopEcRc02142 mutants actually survive and recover from sedation.
Scer\GAL4unspecified-mediated expression of DopEcRdsRNA.Scer\UAS in a DopEcRc02142 background results in defects in the initiation of male courtship and in olfactory conditioning.
Homozygotes reach adulthood and have no gross morphological defects. The short- and long-latency thresholds of the giant-fiber pathway in heterozygous, homozygous and DopEcRc02142/Df(3L)ED4341 flies is indistinguishable from that of controls. However, the refractory period is significantly reduced in homozygotes compared to controls and homozygotes and DopEcRc02142/Df(3L)ED4341 flies show a delay in habituation of the giant fiber pathway. Homozygous and DopEcRc02142/Df(3L)ED4341 males lack 30-minute courtship memory in a courtship conditioning assay, although homozygotes show significant memory immediately after conditioning.
Unlike in wild type flies, DopEcRc02142 mutants do not show an increased sugar sensitive proboscis extension reflex (PER) in response to either L-Dopa feeding or 6 hours of wet starvation. The loss of response to L-Dopa is observed 48 hours after feeding, whereas the starvation response is observed at 6 hours, but is not seen after 24 hours.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
The increased resistance to ethanol-induced sedation characteristic for DopEcRc02142 homozygous adults is significantly suppressed by expression of EgfrKK100051 under the control of the Scer\GAL4elav.PU driver in the mutant background.
DopEcRc02142 restores 30-minute courtship memory in a courtship conditioning assay in dnc1 and dnc2 males.
The reduction in nighttime sleep seen in flies expressing TrpA1Scer\UAS.(B).cKa under the control of Scer\GAL4ple.PF at 27[o]C is not suppressed by DopEcRc02142/DopEcRc02142.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
DopEcRc02142 adults show a restored ethanol-induced disinhibition after adulthood-only, but not development-only, expression of DopEcRScer\UAS.cAa under the control of Scer\GAL4Mef2.247, as shown by their normal increasing frequency of inter-male courtship upon continual exposure (temporal regulation of expression is achieved by the use of Gal80[ts]). A similar rescue is achieved upon expression under the control of Scer\GAL4αTub84B.Switch.PK and RU486 treatment during adulthood.
Expression of DopEcRScer\UAS.cIb under the control of either Scer\GAL4DopEcR.588, Scer\GAL4c747 or Scer\GAL4c739 rescues courtship memory in DopEcRc02142 males a courtship conditioning assay. Expression of DopEcRScer\UAS.cIb under the control of Scer\GAL4Tab2-201Y does not rescue courtship memory in DopEcRc02142 males a courtship conditioning assay.
Expression of DopEcRScer\UAS.cIa under the control of Scer\GAL4Gr5a.8.5 rescues the loss of the sugar-sensitive proboscis extension reflex (PER) seen in DopEcRc02142 mutants in response to 6 hours of wet starvation.
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (11)