FB2025_01 , released February 20, 2025
Allele: Dmel\incf00285
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General Information
Symbol
Dmel\incf00285
Species
D. melanogaster
Name
FlyBase ID
FBal0162225
Feature type
allele
Associated gene
Dmel\inc
Associated Insertion(s)
PBac{WH}incf00285
Carried in Construct
Also Known As
inc2, f00285
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Pfeiffenberger and Allada, 2012, Stavropoulos and Young, 2011)
Mutagen
piggyBac activity
(Bloomington Drosophila Stock Center, 2004.11.6)
Progenitor genotype
Associated Insertion(s)
PBac{WH}incf00285
Cytology
Description

Insertion in the 5' untranslated region. The inserted PBac{WH} element contains Scer\UAS regulatory sequences that are in the correct orientation to over-express inc in the presence of a Scer\GAL4 driver.

(Stavropoulos and Young, 2011)
Allele components
Component
Use(s)
Inserted element
PBac{WH}
Regulatory region(s)
UAS
Also carries
FRT
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      incf00285 mutant third instar larvae show increased number of boutons on neuromuscular junction. Postsynaptic muscle recordings reveal altered synaptic transmission in inc1/incf00285 larvae: the amplitude of spontaneous miniature postsynaptic potentials is comparable to controls but their frequency is reduced, the amplitude of evoked potentials is decreased as is the quantal content.

      (Li et al., 2017)

      Mutant flies show a reduction in sleep duration compared to controls. Sleep is reduced during both light and dark periods, but the sleep levels of the mutant flies drop in anticipation of light-dark and dark-light conditions, suggesting that the circadian clock is intact. The mutant flies show a reduction in sleep bout length accompanied by an increase in sleep bout number and show an increased latency to sleep after lights-off. The mutant flies show a modest reduction in activity during wakefulness. The mutant flies show reduced sleep homeostasis, lacking a detectable sleep rebound after 12 or 24 hours of sleep deprivation. The mutant flies are more easily woken by a rotational stimulus compared to wild type controls.

      A proportion of mutant flies lack either an α- or β-lobe in the mushroom body.

      (Pfeiffenberger and Allada, 2012)

      These homozygotes are also short lived compared to controls.

      (Stavropoulos and Young, 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      incf00285 has short lived | male phenotype, suppressible by brp+t83.4

      (Huang et al., 2022)
      NOT suppressed by
      Statement
      Reference

      incf00285 has abnormal sleep phenotype, non-suppressible by Cdk1[+]/Cdk1GT-000294

      (Afonso et al., 2015)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of Cdk1GT-000294 does not suppress the sleep defects seen in incf00285 mutant flies.

      (Afonso et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      inc1

      (Stavropoulos and Young, 2011)
      Rescued by

      incf00285 is rescued by Scer\GAL4ChAT.7.4, incf00285

      (Pfeiffenberger and Allada, 2012)

      incf00285 is rescued by Scer\GAL4inc.PP, incf00285

      (Pfeiffenberger and Allada, 2012)

      incf00285 is rescued by Scer\GAL4Act5C.PI, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is rescued by Scer\GAL4αTub84B.PL, incf00285

      (Stavropoulos and Young, 2011)
      Partially rescued by

      incf00285 is partially rescued by Scer\GAL4c309, incf00285

      (Pfeiffenberger and Allada, 2012)

      incf00285 is partially rescued by Scer\GAL430Y, incf00285

      (Pfeiffenberger and Allada, 2012)

      incf00285 is partially rescued by Scer\GAL4G0451, incf00285

      (Pfeiffenberger and Allada, 2012)

      incf00285 is partially rescued by Scer\GAL4nSyb.PS, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is partially rescued by Scer\GAL4qvr.PW, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is partially rescued by Scer\GAL4ChAT.7.4, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is partially rescued by Scer\GAL4Toll-6-D42, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is partially rescued by Scer\GAL4inc.PS, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is partially rescued by Scer\GAL4VGlut1.PS, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is partially rescued by Scer\GAL4c309, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is partially rescued by Scer\GAL4CenB1A-Mai301, incf00285

      (Stavropoulos and Young, 2011)
      Not rescued by

      incf00285 is not rescued by Scer\GAL4Mef2.247, incf00285

      (Pfeiffenberger and Allada, 2012)

      incf00285 is not rescued by Scer\GAL4c739, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4tim.PE, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4cry.PU, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4Ilp2.PR, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL430Y, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL417d, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4ey-OK107, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4Tab2-201Y, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4Alp4-c507, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4Tdc2.PC, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4ple.PF, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4Mef2.PR, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4how-24B, incf00285

      (Stavropoulos and Young, 2011)

      incf00285 is not rescued by Scer\GAL4repo.PU, incf00285

      (Stavropoulos and Young, 2011)
      Rescues

      Scer\GAL4ChAT.7.4, incf00285 rescues incf00285

      (Pfeiffenberger and Allada, 2012)

      Scer\GAL4inc.PP, incf00285 rescues incf00285

      (Pfeiffenberger and Allada, 2012)

      Scer\GAL4Act5C.PI, incf00285 rescues incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4αTub84B.PL, incf00285 rescues incf00285

      (Stavropoulos and Young, 2011)
      Partially rescues

      Scer\GAL4c309, incf00285 partially rescues incf00285

      (Pfeiffenberger and Allada, 2012)

      Scer\GAL430Y, incf00285 partially rescues incf00285

      (Pfeiffenberger and Allada, 2012)

      Scer\GAL4G0451, incf00285 partially rescues incf00285

      (Pfeiffenberger and Allada, 2012)

      Scer\GAL4nSyb.PS, incf00285 partially rescues incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4qvr.PW, incf00285 partially rescues incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4ChAT.7.4, incf00285 partially rescues incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4Toll-6-D42, incf00285 partially rescues incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4inc.PS, incf00285 partially rescues incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4VGlut1.PS, incf00285 partially rescues incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4c309, incf00285 partially rescues incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4CenB1A-Mai301, incf00285 partially rescues incf00285

      (Stavropoulos and Young, 2011)
      Fails to rescue

      Scer\GAL4Mef2.247, incf00285 fails to rescue incf00285

      (Pfeiffenberger and Allada, 2012)

      Scer\GAL4c739, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4tim.PE, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4cry.PU, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4Ilp2.PR, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL430Y, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL417d, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4ey-OK107, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4Tab2-201Y, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4Alp4-c507, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4Tdc2.PC, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4ple.PF, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4Mef2.PR, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4how-24B, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)

      Scer\GAL4repo.PU, incf00285 fails to rescue incf00285

      (Stavropoulos and Young, 2011)
      Comments

      Expression of inc under the control of either Scer\GAL4inc.PP or Scer\GAL4Cha.7.4 from the Scer\UAS regulatory sequences present in the PBac{WH}incf00285 insertion rescue the defects in sleep duration seen in incf00285 mutants, while expression under the control of either Scer\GAL430Y, Scer\GAL4c309 or Scer\GAL4G0451 partially rescues the sleep duration defects. Expression under the control of Scer\GAL4Mef2.247 does not rescue the sleep duration defects. Expression under the control of either Scer\GAL430Y or Scer\GAL4Cha.7.4 rescues the sleep homeostasis defects of incf00285 mutants.

      Expression of inc under the control of Scer\GAL4elav.Switch.PO during development (using RU486 to control the timing of expression) from the Scer\UAS regulatory sequences present in the PBac{WH}incf00285 insertion partially rescues the sleep defects of incf00285 mutants, but expression under the control of Scer\GAL4elav.Switch.PO during the adult stage alone does not result in significant rescue of the sleep defects.

      DATfmn incf00285 double mutants do not sleep significantly less than either single mutant.

      (Pfeiffenberger and Allada, 2012)

      Expression of inc from the PBac{WH}incf00285 insertion (using either Scer\GAL4Act5C.PI or Scer\GAL4αTub84B.PL) completely rescues the reduction in sleep per day which is seen in incf00285 homozygotes.

      Expression of inc from the PBac{WH}incf00285 insertion using any one of the following Scer\GAL4 drivers partially rescues the reduction in sleep per day which is seen in incf00285 homozygotes: Scer\GAL4n-syb.PS, Scer\GAL4qvr.PW, Scer\GAL4Cha.7.4, Scer\GAL4D42, Scer\GAL4inc.PS, Scer\GAL4VGlut.PS, Scer\GAL4c309 and Scer\GAL4cenB1A-Mai301.

      Expression of inc from the PBac{WH}incf00285 insertion using any one of the following Scer\GAL4 drivers does not rescue the reduction in sleep per day which is seen in incf00285 homozygotes: Scer\GAL4c739, Scer\GAL4tim.PE, Scer\GAL4cry.PU, Scer\GAL4Ilp2.PR, Scer\GAL430Y, Scer\GAL417d, Scer\GAL4ey-OK107, Scer\GAL4Tab2-201Y, Scer\GAL4Aph-4-c507, Scer\GAL4Tdc2.PC, Scer\GAL4ple.PF, Scer\GAL4Mef2.PR, Scer\GAL4how-24B or Scer\GAL4repo.PU.

      (Stavropoulos and Young, 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      References (15)