FB2025_01 , released February 20, 2025
Allele: Dmel\PQBP1PL00109
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General Information
Symbol
Dmel\PQBP1PL00109
Species
D. melanogaster
Name
FlyBase ID
FBal0162527
Feature type
allele
Associated gene
Dmel\PQBP1
Associated Insertion(s)
PBac{GAL4D,EYFP}PQBP1PL00109
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Wan et al., 2015, Tamura et al., 2010)
Mutagen
P-element activity
(Gene Disruption Project members, 2001-)
Progenitor genotype
Associated Insertion(s)
PBac{GAL4D,EYFP}PQBP1PL00109
Cytology
Description

Insertion in the 5' untranslated region of the first exon.

(Tamura et al., 2010)
Allele components
Component
Use(s)
Inserted element
PBac{GAL4D,EYFP}
Encoded product / tool
GAL4(II-9)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult antennal lobe | surrounding
      (Tamura et al., 2010)
      adult antennal nerve | surrounding
      (Tamura et al., 2010)
      Additional Information
      Statement
      Reference

      PQBP1PL00109 is expressed in the cell bodies in the proximity of the antennal lobe and the antennal nerve. Expression of PQBP1PL00109 surrounds the projection neurons that fasciculate with the antennal nerve and innervate the antennal lobe.

      (Tamura et al., 2010)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Renpenning syndrome
      CEA
      (Wan et al., 2015, Tamura et al., 2010)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  Renpenning syndrome
      is ameliorated by Nmdar1UAS.cMa
      (Tamura et al., 2010)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      PQBP1PL00109 mutant flies exhibit lower electroretinogram amplitudes than wild type flies.

      The photoreceptor cells in newly eclosed PQBP1PL00109 mutant flies have split and disorganised microvilli as well as a reduced rhabdomere size.

      At 50% of pupal development the actin-based microvilli in PQBP1PL00109 mutant photoreceptor cells are irregularly arranged and loosely packed compared to the tightly packed rows in wild type pupae. Microvillus density and length are both reduced at this stage, but elongation is normal at 60-80% pupal development. Proximal to distal elongation of the photoreceptor cells takes place normally but ommatidium length is reduced.

      (Wan et al., 2015)

      Heterozygous and homozygous flies have a significantly shorter average lifespan (25.8 days and 17.5 days respectively) than wild-type controls (33.4 days). The 90% maximum lifespan is also reduced (43 and 32 days respectively, compared to 47 days for wild type).

      (Tamura et al., 2013)

      Homozygous mutants show a significant decrease in performance index within 3 minutes after single training in an olfactory conditioning assay, indicating a defect in learning acquisition. The mutants show defects in 1 and 3 hour memory similar to the defects seen immediately after training.

      Homozygotes have normal olfactory acuity in response to 4-methylcyclohexanol or 3-octanol.

      Homozygous and heterozygous adults show no significant change in mushroom body morphology compared to wild-type controls.

      Projection neurons are morphologically normal in homozygous adults.

      (Tamura et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Nmdar1Scer\UAS.cMa under the control of Scer\GAL4elav.PLu rescues the shortened lifespan of PQBP1PL00109 flies.

      (Tamura et al., 2013)

      The learning defects of PQBP1PL00109 flies are rescued by expression of Nmdar1Scer\UAS.cMa under the control of Scer\GAL4PQBP1-PL00109.

      (Tamura et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      PQBP1PL00109 is rescued by PQBP1UAS.cWa

      (Wan et al., 2015)

      PQBP1PL00109 is rescued by PQBP1UAS.cTa/Scer\GAL4elav.PLu

      (Tamura et al., 2013)

      PQBP1PL00109 is rescued by Scer\GAL4PQBP1-PL00109/PQBP1UAS.cTa

      (Tamura et al., 2010)
      Comments

      Expression of PQBP1Scer\UAS.cWa under the control of Scer\GAL4GMR.PU restores rhabdomere structure and electroretinogram response in newly eclosed PQBP1PL00109 mutant flies.

      (Wan et al., 2015)

      Expression of PQBP1Scer\UAS.cTa under the control of Scer\GAL4elav.PLu rescues the shortened lifespan of PQBP1PL00109 flies.

      (Tamura et al., 2013)

      Expression of PQBP1Scer\UAS.cTa under the control of Scer\GAL4PQBP1-PL00109 completely rescues the learning acquisition defects of PQBP1PL00109 homozygotes.

      Experiments in which the temperature sensitive Scer\GAL80ts.αTub84B allele is used to limit expression of PQBP1Scer\UAS.cTa under the control of Scer\GAL4PQBP1-PL00109 to particular stages show that expression of PQBP1Scer\UAS.cTa during the adult stage is necessary to rescue the learning defects of PQBP1PL00109 homozygotes.

      (Tamura et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG11820PL00109
      PQBP1PL00109
      Name Synonyms
      Secondary FlyBase IDs
        References (5)