FlyBase Allele Report: Dmel\Sin1[e03756]

(Jevtov et al., 2015, Dimitroff et al., 2012, Han et al., 2012, Koike-Kumagai et al., 2009, Hietakangas and Cohen, 2007, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Heterozygous Sin1^e03756 ameliorates the neoplasia seen in the btl::Egfr^{Scer\UAS.T:λ\cI-DD}, Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} brain glioma model when in combination with Df(2R)BSC11.

(Read et al., 2009)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal anterior ventral multidendritic neuron vdaa

(Koike-Kumagai et al., 2009)

cytoplasmic stress granule | larval stage | heat sensitive

(Jevtov et al., 2015)

dendrite | cell autonomous | somatic clone

(Koike-Kumagai et al., 2009)

embryonic/larval hemocyte | larval stage | heat sensitive

(Jevtov et al., 2015)

embryonic/larval neuromuscular junction

(Dimitroff et al., 2012)

larval dorsal multidendritic neuron ddaC

(Koike-Kumagai et al., 2009)

larval dorsal multidendritic neuron ddaC | third instar larval stage

(Han et al., 2012)

larval multidendritic class IV neuron

(Koike-Kumagai et al., 2009)

larval multidendritic class IV neuron (with Df(2R)BSC11)

(Koike-Kumagai et al., 2009)

larval multidendritic class IV neuron | cell autonomous | somatic clone

(Koike-Kumagai et al., 2009)

larval ventral multidendritic neuron vdaB

(Koike-Kumagai et al., 2009)

wing (with Df(2R)BSC11)

(Hietakangas and Cohen, 2007)

Detailed Description

Statement

Reference

Sin1^e03756 mutant adults and larvae exhibit increased heat sensitivity, with absent or reduced mobility as compared to controls upon heat exposure at 37[o]C. In contrast to controls, Sin1^e03756 mutant larvae fail to form stress granules in their hemocytes 2 hours after heat exposure, but stress granule formation in the brain appears unaffected.

(Jevtov et al., 2015)

Sin1^e03756 mutants show a significantly smaller synapse at the larval neuromuscular junction compared to controls.

(Dimitroff et al., 2012)

Sin1^e03756 homozygous mutant third instar larvae show a mild but statistically significant increase in the proportion of dorsal midline ddaC dendrite length that is enclosed within the epidermis rather than attached to the ECM. Dendrite crossing is seen in these mutants, and the majority of these crossings are between enclosed dendrites and dendrites attached to the ECM and are thus non-contacting.

(Han et al., 2012)

Homozygotes and Sin1^e03756/Df(2R)BSC11 animals show a robust dendritic tiling defect in class IV dendrite arborization (da) neurons, with approximately 10% of dendritic branches crossing one another. In addition, the total number of dendrite branches is reduced to approximately 80% of wild type.

Single-cell dorsal class IV da neuron clones that are homozygous for Sin1^e03756 (in a heterozygous background) show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type.

The v'ada and vdaB dendrites often invade neighbouring dendritic fields in homozygous mutants, in contrast to what is seen in wild-type controls.

(Koike-Kumagai et al., 2009)

Sin1^e03756/Df(2R)BSC11 mutant third instar larval brains display normal numbers of glia.

(Read et al., 2009)

Sin1^e03756 flies are normal in appearance and show a very modest delay of development.

Sin1^e03756/Df(2R)BSC11 flies show a modest reduction in tissue growth (wing area is reduced) compared to controls.

(Hietakangas and Cohen, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Sin1^e03756 has abnormal heat stress response | adult stage phenotype, non-enhanceable by rictor^305A

(Jevtov et al., 2015)

Suppressed by

Statement

Reference

Sin1^e03756 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by mew^UAS.cMBa/mys^UAS.cDa/Scer\GAL4^ppk.PH

(Han et al., 2012)

Sin1^e03756 has abnormal neuroanatomy phenotype, suppressible | partially by trc^UAS.cUa/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

Sin1^e03756 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^ppk.PG/trc^{UAS.Tag:Myr(Src64B)}

(Koike-Kumagai et al., 2009)

NOT suppressed by

Statement

Reference

Sin1^e03756 has abnormal neuroanatomy phenotype, non-suppressible by trc^{T449A.UAS.Tag:Myr(Src64B)}/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

Suppressor of

Statement

Reference

Df(2R)BSC11/Sin1^e03756 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Df(2R)BSC11/Sin1^e03756 is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

NOT Suppressor of

Statement

Reference

Sin1^e03756/Sin1^e03756 is a non-suppressor of abnormal neuroanatomy phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

Other

Statement

Reference

Df(2R)Sema2b-C4, Sin1^e03756 has abnormal neuroanatomy | third instar larval stage phenotype

(Meltzer et al., 2016)

Sin1[+]/Sin1^e03756, mTor^ΔP has abnormal neuroanatomy | dominant phenotype

(Koike-Kumagai et al., 2009)

Sin1^e03756, trc¹/trc[+] has abnormal neuroanatomy phenotype

(Koike-Kumagai et al., 2009)

Sin1[+]/Sin1^e03756, rictor^Δ2 has abnormal neuroanatomy | dominant phenotype

(Koike-Kumagai et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sin1^e03756 has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible by mew^UAS.cMBa/mys^UAS.cDa/Scer\GAL4^ppk.PH

(Han et al., 2012)

Sin1^e03756 has larval dorsal multidendritic neuron ddaC phenotype, suppressible | partially by trc^UAS.cUa/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

Sin1^e03756 has larval dorsal multidendritic neuron ddaC phenotype, suppressible by Scer\GAL4^ppk.PG/trc^{UAS.Tag:Myr(Src64B)}

(Koike-Kumagai et al., 2009)

NOT suppressed by

Statement

Reference

Sin1^e03756 has larval dorsal multidendritic neuron ddaC phenotype, non-suppressible by trc^{T449A.UAS.Tag:Myr(Src64B)}/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

Suppressor of

Statement

Reference

Sin1^e03756/Sin1^e03756 is a suppressor of embryonic/larval neuromuscular junction phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

Df(2R)BSC11/Sin1^e03756 is a suppressor of glial cell | increased number | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Df(2R)BSC11/Sin1^e03756 is a suppressor of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

NOT Suppressor of

Statement

Reference

Sin1^e03756/Sin1^e03756 is a non-suppressor of photoreceptor cell R7 phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

Sin1^e03756/Sin1^e03756 is a non-suppressor of photoreceptor cell R8 phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

Other

Statement

Reference

Df(2R)Sema2b-C4, Sin1^e03756 has larval multidendritic class IV neuron | third instar larval stage phenotype

(Meltzer et al., 2016)

Df(2R)Sema2b-C4, Sin1^e03756 has dendrite | third instar larval stage phenotype

(Meltzer et al., 2016)

Sin1[+]/Sin1^e03756, mTor^ΔP has dendrite phenotype

(Koike-Kumagai et al., 2009)

Sin1[+]/Sin1^e03756, rictor^Δ2 has dendrite phenotype

(Koike-Kumagai et al., 2009)

Sin1[+]/Sin1^e03756, rictor^Δ2 has larval multidendritic class IV neuron phenotype

(Koike-Kumagai et al., 2009)

Sin1[+]/Sin1^e03756, mTor^ΔP has larval dorsal multidendritic neuron ddaC phenotype

(Koike-Kumagai et al., 2009)

Sin1^e03756, trc¹/trc[+] has dendrite phenotype

(Koike-Kumagai et al., 2009)

Sin1^e03756, trc¹/trc[+] has larval multidendritic class IV neuron phenotype

(Koike-Kumagai et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The level of non-contacting dendrite crossing in class IV dendrite arborizing larval neurons is significantly increased in Sin1^e03756;Df(2R)Sema-2b-C4 double mutants compared to either Sin1^e03756/+ or Df(2R)Sema-2b-C4/+ heterozygotes.

(Meltzer et al., 2016)

Sin1^e03756, rictor^305A double mutants exhibit a similar level of heat sensitivity as observed in Sin1^e03756 single mutants.

(Jevtov et al., 2015)

The axon guidance phenotypes of Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu flies are not significantly rescued in a Sin1^e03756/Sin1^e03756 background.

The Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu synapse overgrowth phenotype is significantly rescued in a Sin1^e03756/Sin1^e03756 background.

(Dimitroff et al., 2012)

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} under the control of Scer\GAL4^ppk.PH rescues the increase in epidermally enclosed dendrites seen in the dorsal midline of Sin1^e03756 mutants. The dendritic crossing seen in Sin1^e03756 mutants is also largely rescued. The dendritic crossings that remain in the rescue flies are between two ECM attached dendrite, thus they are making contact. No dendritic crossing is seen between dendrites on the v'ada\vdaB neuron interface. However the few that are seen are rescued by overexpression of mys and mew.

(Han et al., 2012)

rictor^Δ2/+ ; Sin1^e03756/+ double heterozygotes have a dendritic tiling defect in class IV dendrite arborization (da) neurons, with a significantly higher number of dendritic branches crossing one another compared to wild type (neither single heterozygote shows this phenotype).

rictor^Δ2 ; Sin1^e03756 double homozygotes show dendritic tiling defects in class IV da neurons that are indistinguishable from the single homozygotes.

The dendritic branches of ddaC neurons in Tor^ΔP/+ ; Sin1^e03756/+ double heterozygous larvae show increased crossing over of each other, indicating a tiling defect.

Class IV da neurons of Sin1^e03756/+ ; trc¹/+ double heterozygous larvae show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type.

Expression of trc^Scer\UAS.cUa under the control of Scer\GAL4^ppk.PG slightly reduces the dendritic tiling defects seen in ddaC neurons in Sin1^e03756 animals.

Expression of trc^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^ppk.PG significantly rescues the dendritic tiling defects seen in ddaC neurons in Sin1^e03756 animals.

Expression of trc^{T449A.Scer\UAS.T:Myr1} under the control of Scer\GAL4^ppk.PG does not rescue the dendritic tiling defects seen in ddaC neurons in Sin1^e03756 animals.

(Koike-Kumagai et al., 2009)

Sin1^e03756/Df(2R)BSC11 almost completely suppresses the glial neoplasia seen in third instar larvae when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU.

(Read et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sin1^e03756 is rescued by Sin1^UAS.Tag:FLAG/Scer\GAL4^unspecified

(Koike-Kumagai et al., 2009)

Sin1^e03756 is rescued by Sin1^UAS.Tag:FLAG/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

Comments

Class IV neuron-specific expression of Sin1^{Scer\UAS.T:Zzzz\FLAG} largely rescues the dendritic phenotypes of single-cell Sin1^e03756 class IV dendrite arborization neuron clones.

Expression of Sin1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ppk.PG largely rescues the tiling defects seen in v'ada and vdaB neurons of Sin1^e03756 mutants.

(Koike-Kumagai et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

18188

w¹¹¹⁸; PBac{RB}Sin1^e03756

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG10105^e03756

Sin1^e03756

(Han et al., 2012)

Sin^e03756

(Zheng and Sehgal, 2010)

dSin1 ^e03756

(Read et al., 2009)

sin1^PBac

(Koike-Kumagai et al., 2009)

sin1^e03756

(Meltzer et al., 2016, Hietakangas and Cohen, 2007)

Name Synonyms

Secondary FlyBase IDs

References (11)

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