Allele Dmel\Aos1^c06048

General Information
Symbol	Dmel\Aos1^c06048	Species	D. melanogaster
Name		FlyBase ID	FBal0162805
Feature type	allele	Associated gene	Dmel\Aos1

Map ( GBrowse )	Untitled Document
Allele class	amorphic allele - molecular evidence
Mutagen	piggyBac transposase
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - molecular evidence (Kanakousaki and Gibson, 2012)
Mutagen	piggyBac transposase (Bloomington Drosophila Stock Center, 2004.11.6)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:8,259,494..8,259,494 (Bloomington Drosophila Stock Center, 2004.11.6, Geng and MacDonald, 2007, Kanakousaki and Gibson, 2012)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion in the 5' UTR, 41bp upstream of the ATG codon. (Kanakousaki and Gibson, 2012)
Caused by insertion	PBac{PB}Aos1^c06048 (Bloomington Drosophila Stock Center, 2004.11.6, Geng and MacDonald, 2007, Kanakousaki and Gibson, 2012)
Cytology
Phenotypic Data
Phenotypic Class
	cell lethal \| somatic clone \| third instar larval stage (Kanakousaki and Gibson, 2012) cell lethal \| somatic clone - Minute background \| third instar larval stage (Kanakousaki and Gibson, 2012) decreased cell number \| somatic clone \| third instar larval stage (Kanakousaki and Gibson, 2012) developmental rate defective (Kanakousaki and Gibson, 2012) increased cell death \| somatic clone \| third instar larval stage (Kanakousaki and Gibson, 2012) increased cell death \| somatic clone - Minute background \| third instar larval stage (Kanakousaki and Gibson, 2012) lethal \| P-stage (Kanakousaki and Gibson, 2012) lethal \| P-stage (with Df(3R)ED5554) (Kanakousaki and Gibson, 2012) lethal \| P-stage (with Df(3R)ED5591) (Kanakousaki and Gibson, 2012) melanotic mass phenotype \| third instar larval stage (Kanakousaki and Gibson, 2012) mitotic cell cycle defective \| somatic clone \| third instar larval stage (Kanakousaki and Gibson, 2012)
Phenotype Manifest In
	imaginal disc (with Df(3R)ED5554) (Kanakousaki and Gibson, 2012) imaginal disc (with Df(3R)ED5591) (Kanakousaki and Gibson, 2012) imaginal disc \| larval stage (Kanakousaki and Gibson, 2012) imaginal disc \| somatic clone \| third instar larval stage (Kanakousaki and Gibson, 2012)
Detailed Description
	Statement Reference Homozygous third instar larvae occasionally have melanotic tumours. Homozygotes show developmental delay (puparium formation is delayed relative to wild type) and highly penetrant early pupal lethality. Imaginal discs are missing in homozygous larvae. Aos1[c06048]/Df(3R)ED5554 and Aos1[c06048]/Df(3R)ED5591 animals show loss of imaginal discs and pupal lethality. Homozygous clones in the wing disc are small, fragmented and show high levels of cell death 3 days after clone induction. The defects in growth and viability of homozygous clones are not rescued if the clones are induced in a Minute background. Cells in homozygous clones in the imaginal discs accumulate in G2/M of the cell cycle. EdU incorporation in the salivary glands of mutant early third instar larvae appears normal. (Kanakousaki and Gibson, 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Aos1^c06048 has increased cell death \| somatic clone \| third instar larval stage phenotype, suppressible by Scer\GAL4^Act.PU/BacA\p35^Scer\UAS.cHa (Kanakousaki and Gibson, 2012)
NOT suppressed by
Statement Reference Aos1^c06048 has decreased cell number \| somatic clone \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^Act.PU/BacA\p35^Scer\UAS.cHa (Kanakousaki and Gibson, 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[Act.PU] in homozygous Aos1[c06048] clones in the wing disc suppresses the increased cell death seen in these clones, but does not rescue the growth defect, as the double mutant clones are still smaller in size than control clones. The rescued cells appear significantly larger than control cells are are partially extruded from the epithelial layer, leaving only one or two thin membraneous processes attached to the apical epithelial surface. (Kanakousaki and Gibson, 2012)
Complementation & Rescue Data
Rescued by	Aos1^c06048 is rescued by Aos1^+t2.9 (Kanakousaki and Gibson, 2012) Aos1^c06048 is rescued by Aos1^Scer\UAS.cKa/Scer\GAL4^Act.PU (Kanakousaki and Gibson, 2012)
Comments	Expression of Aos1[Scer\UAS.cKa] under the control of Scer\GAL4[Act.PU] in the clone rescues the cell lethality of Aos1[c06048] clones. (Kanakousaki and Gibson, 2012)
Stocks ( 2 )
Bloomington	17744 w¹¹¹⁸; PBac{PB}Aos1^c06048/TM6B, Tb¹
Harvard	- PBac{PB}Aos1^c06048
Notes on Origin
Discoverer

Comments
	Precise excision of the insertion reverts the mutant phenotype. (Kanakousaki and Gibson, 2012)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Aos1^c06048 aos1^c06048 (Kanakousaki and Gibson, 2012)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Kanakousaki and Gibson, 2012, Development 139(15): 2751--2762 A differential requirement for SUMOylation in proliferating and non-proliferating cells during Drosophila development. [FBrf0218825] Geng and MacDonald, 2007, Fly 1(5): 259--267 Identification of genes that influence gurken expression. [FBrf0205043]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]

Allele Dmel\Aos1c06048

Allele Dmel\Aos1^c06048