|Feature type||allele||Associated gene||Dmel\Aos1|
|Map ( GBrowse )||
|Allele class||amorphic allele - molecular evidence|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Insertion in the 5' UTR, 41bp upstream of the ATG codon.
|Caused by insertion|
|Phenotype Manifest In|
Homozygous third instar larvae occasionally have melanotic tumours. Homozygotes show developmental delay (puparium formation is delayed relative to wild type) and highly penetrant early pupal lethality. Imaginal discs are missing in homozygous larvae. Aos1[c06048]/Df(3R)ED5554 and Aos1[c06048]/Df(3R)ED5591 animals show loss of imaginal discs and pupal lethality. Homozygous clones in the wing disc are small, fragmented and show high levels of cell death 3 days after clone induction. The defects in growth and viability of homozygous clones are not rescued if the clones are induced in a Minute background. Cells in homozygous clones in the imaginal discs accumulate in G2/M of the cell cycle. EdU incorporation in the salivary glands of mutant early third instar larvae appears normal.
|NOT suppressed by|
|Phenotype Manifest In|
Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[Act.PU] in homozygous Aos1[c06048] clones in the wing disc suppresses the increased cell death seen in these clones, but does not rescue the growth defect, as the double mutant clones are still smaller in size than control clones. The rescued cells appear significantly larger than control cells are are partially extruded from the epithelial layer, leaving only one or two thin membraneous processes attached to the apical epithelial surface.
|Complementation & Rescue Data|
|Stocks ( 2 )|
|Notes on Origin|
Precise excision of the insertion reverts the mutant phenotype.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 4 )|
|Personal communication to FlyBase|