FlyBase Allele Report: Dmel\Diap1[RNAi.UAS]

General Information

Symbol

Dmel\Diap1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0175406

Feature type

allele

Associated gene

Dmel\Diap1

Associated Insertion(s)

Carried in Construct

P{UAS-Diap1.RNAi}

Also Known As

UAS-diap1-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Huh et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Huh et al., 2004)

Mutagen

in vitro construct

(Huh et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Diap1.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of Diap1 sequences, which are arranged such that this results in the production of dsRNA corresponding to Diap1 sequences.

(Huh et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Diap1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PU

(Merkling et al., 2019)

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Merkling et al., 2019)

increased cell death, with Scer\GAL4^34B

(Choi et al., 2006)

increased cell death, with Scer\GAL4^GMR.PF

(Merkling et al., 2015, Chou et al., 2007, Meyer et al., 2006)

increased cell death, with Scer\GAL4^GMR.PU

(McCann et al., 2011)

increased cell death | adult stage, with Scer\GAL4^GMR.PU

(Merkling et al., 2019)

visible, with Scer\GAL4^GMR.PF

(Kondo et al., 2009)

visible, with Scer\GAL4^GMR.PU

(McCann et al., 2011, Arya et al., 2010)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Merkling et al., 2015, Kondo et al., 2009, Chou et al., 2007, Meyer et al., 2006, Muro et al., 2006)

eye, with Scer\GAL4^GMR.PU

(Merkling et al., 2019, McCann et al., 2011, Arya et al., 2010)

pigment cell, with Scer\GAL4^GMR.PF

(Muro et al., 2006)

Detailed Description

Statement

Reference

Expression of Diap1^dsRNA.UAS under the control of Scer\GAL4^GMR.PU leads to increased apoptosis in the eye visualized by loss of pigmentation and reduced eye size when compared to controls.

(Merkling et al., 2019)

Expression of Diap1^{dsRNA.Scer\UAS} using the eye-specific driver Scer\GAL4^GMR.PF leads to severe apoptosis in the developing eye. Consequently, adult Scer\GAL4^GMR.PF>Diap1^{dsRNA.Scer\UAS} flies display reduced eye size, roughening of the eye surface, and loss of pigmentation.

(Merkling et al., 2015)

Expression of th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in significantly smaller eyes because of increased apoptosis.

(McCann et al., 2011)

Scer\GAL4^GMR.PU-driven expression of th^{dsRNA.Scer\UAS} results in eye degeneration.

(Arya et al., 2010)

Expression of Diap1^{dsRNA.Scer\UAS} in the developing eye under the control of Scer\GAL4^GMR.PF results in a smaller, less-pigmented eye than wild-type, due to an increase in apoptosis.

(Chou et al., 2007)

Expression of th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Crz does not cause premature cell death.

Expression of th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^34B results in premature cell death.

(Choi et al., 2006)

Expression of th^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF, results in small eyes due to high levels of cell death by apoptosis.

(Meyer et al., 2006)

Flies that express th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF have moderately small eyes, with an extensive loss of pigment.

(Muro et al., 2006)

Expression of th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 in the third larval instar wing disc does not result in increased cell proliferation in the domain of Scer\GAL4^ptc-559.1 expression.

(Huh et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU has abnormal eye color phenotype, non-enhanceable by Sgp^KK106304, Scer\GAL4^GMR.PU

(Merkling et al., 2019)

Suppressed by

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible by P{CH1226-68E13}

(Kondo et al., 2009)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by FHV\B2^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by NDV\B2^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by VV\E3L^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by Dcr-2[+]/Dcr-2^L811fsX

(Meyer et al., 2006)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by AGO2^51B/AGO2^51B

(Meyer et al., 2006)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by AGO1^k08121/AGO1[+]

(Meyer et al., 2006)

NOT suppressed by

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU has abnormal eye color phenotype, non-suppressible by Sgp^KK106304, Scer\GAL4^GMR.PU

(Merkling et al., 2019)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, non-suppressible by G9a^DD2

(Merkling et al., 2015)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU has increased cell death phenotype, non-suppressible by Atx2^GD11562, Scer\GAL4^GMR.PU

(McCann et al., 2011)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by Atx2^GD11562, Scer\GAL4^GMR.PU

(McCann et al., 2011)

Diap1^RNAi.UAS, Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by Hsp60D^RNAi.Sym.UAS, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has lethal phenotype, non-suppressible by Hsp60D^RNAi.Sym.UAS, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, non-suppressible by TBSV\P19^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, non-suppressible by Ivir\NS1^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Enhancer of

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Other

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has lethal phenotype

(Arya et al., 2010)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Sgp^KK106304, Scer\GAL4^GMR.PU

(Merkling et al., 2019)

Suppressed by

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by P{CH1226-68E13}

(Kondo et al., 2009)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by FHV\B2^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by NDV\B2^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by VV\E3L^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Dcr-2[+]/Dcr-2^L811fsX

(Meyer et al., 2006)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by AGO2^51B/AGO2^51B

(Meyer et al., 2006)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by AGO1^k08121/AGO1[+]

(Meyer et al., 2006)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Drice^Δ1

(Muro et al., 2006)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has pigment cell phenotype, suppressible by Drice^Δ1

(Muro et al., 2006)

NOT suppressed by

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Sgp^KK106304, Scer\GAL4^GMR.PU

(Merkling et al., 2019)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by G9a^DD2

(Merkling et al., 2015)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Atx2^GD11562, Scer\GAL4^GMR.PU

(McCann et al., 2011)

Diap1^RNAi.UAS, Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU has ommatidium phenotype, non-suppressible by Hsp60D^RNAi.Sym.UAS, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by TBSV\P19^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Diap1^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Ivir\NS1^UAS.HM, Scer\GAL4^GMR.PF

(Chou et al., 2007)

Enhancer of

Statement

Reference

Diap1^RNAi.UAS, Scer\GAL4^GMR.PU is an enhancer of ommatidium phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of AGO2^GD17090 suppresses the cell death phenotype seen in the eyes of flies expressing th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

Expression of Atx2^GD11562 does not suppress the cell death phenotype seen in the eyes of flies expressing th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

(McCann et al., 2011)

A Dcr-2^L811fsX/+ background partially suppresses the small eye phenotype that is seen when th^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

In flies homozygous for AGO2^51B, the Scer\GAL4^GMR.PF>th^{dsRNA.Scer\UAS} small eye phenotype is completely suppressed.

The small eye phenotype of Scer\GAL4^GMR.PF>th^{dsRNA.Scer\UAS} flies is mildly suppressed by AGO1^k08121/+.

(Meyer et al., 2006)

The small eye phenotype of Scer\GAL4^GMR.PF>th^{dsRNA.Scer\UAS} flies is partially suppressed in an Ice^Δ1 background.

(Muro et al., 2006)

Coexpression of th^{dsRNA.Scer\UAS} and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 in the third larval instar wing disc does not result in increased cell proliferation in the domain of Scer\GAL4^ptc-559.1 expression.

(Huh et al., 2004)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsp60D^{dsRNA.Sym.Scer\UAS} via Scer\GAL4^GMR.PU fails to suppress the eye degeneration in Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} and th^{dsRNA.Scer\UAS} expressing flies.

The pharate stage lethality due to co-expression of th^{dsRNA.Scer\UAS} and Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} via Scer\GAL4^GMR.PU is not suppressed by simultaneous expression of Hsp60D^{dsRNA.Sym.Scer\UAS}.

Co-expression of th^{dsRNA.Scer\UAS} via Scer\GAL4^GMR.PU in Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1}-expressing flies results in enhanced eye degeneration with reduction in eye size.

Co-expression of th^{dsRNA.Scer\UAS} via Scer\GAL4^GMR.PU in Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}-expressing flies results in death at pharate stage.

(Arya et al., 2010)

The rough eye phenotype of flies expressing th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF is rescued if they are carrying the P{CH1226-68E13} construct (which contains D. pseudoobscura pseudoobscura fosmid sequence that includes the region orthologous to th).

(Kondo et al., 2009)

Eye apoptosis observed in Diap1^{dsRNA.Scer\UAS} Scer\GAL4^GMR.PF mutants can be suppressed through co-expression of BacA\p35^GMR.PH.

Expression of FHV\B2^Scer\UAS.HM can suppress the small, rough eye induced by expression of Diap1^{dsRNA.Scer\UAS} (both under the control of Scer\GAL4^GMR.PF).

Expression of Zzzz\B2^Scer\UAS.HM can suppress the small, rough eye induced by expression of Diap1^{dsRNA.Scer\UAS} (both under the control of Scer\GAL4^GMR.PF).

Eye size and patterning are slightly improved when Zzzz\E3L^Scer\UAS.HM is co-expressed in Diap1^{dsRNA.Scer\UAS} flies (both under the control of Scer\GAL4^GMR.PF).

Expression of Zzzz\p19^Scer\UAS.HM in the eye fails to suppress the small, rough eye phenotype induced by expression of Diap1^{dsRNA.Scer\UAS} (both UAS constructs under the control of Scer\GAL4^GMR.PF).

Expression of Ivir\NS1^Scer\UAS.HM in the eye fails to suppress the small, rough eye phenotype induced by expression of Diap1^{dsRNA.Scer\UAS} (both UAS constructs under the control of Scer\GAL4^GMR.PF).

(Chou et al., 2007)

Complementation and Rescue Data

Rescued by

Diap1^RNAi.UAS is rescued by Scer\GAL4^34B/Diap1^UAS.cHa

(Choi et al., 2006)

Comments

Expression of th^Scer\UAS.cHa in the salivary glands under the control of Scer\GAL4^34B rescues programmed cell death in salivary glands expressing th^{dsRNA.Scer\UAS}.

(Choi et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Diap1^RNAi.UAS

Diap1^{dsRNA.Scer\UAS}

Diap1^dsRNA.UAS

th^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

Open Close

General Information
Nature of the Allele
Expression Data
Human Disease Associations
Phenotypic Data
Interactions
Complementation and Rescue Data
Images
Stocks
Notes On Origin
Crossreferences
Synonyms and Secondary IDs
References