Allele Dmel\sra^GS3168

General Information
Symbol	Dmel\sra^GS3168	Species	D. melanogaster
Name		FlyBase ID	FBal0175437
Feature type	allele	Associated gene	Dmel\sra

Map ( GBrowse )	Untitled Document
Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:12,015,338..12,015,338 (Ejima et al., 2004, Takeo et al., 2006)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{GS} insertion at position +2675 (relative to the transcription start site), in an intron. (Ejima et al., 2004)
Caused by insertion	P{GSV1}sra^GS3168 (Ejima et al., 2004, Takeo et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	courtship behavior defective \| female, with Scer\GAL4^sca-537.4 (Ejima et al., 2004) female sterile \| recessive, with Scer\GAL4[-]: (Ejima et al., 2004)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of sra^GS3168 under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-537.4 results in increased ovulation in virgin females compared to control virgin females. Within 30 minutes in a mating assay, only 35% of virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 have mated, compared to 82% of wild-type virgin females. Virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 elicit significantly lower courtship index (CI) levels (CI is the period of time within the observation period that a male displays any element of courtship behaviour) than wild-type virgin females. Virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 have a high extrusion index (EI, the percentage of a time a female shows extrusion behaviour when being courted by a male) which is nearly comparable to that of wild-type mated females. Homozygous females lay fewer eggs than wild type and most of the eggs are arrested at metaphase I of meiosis. Spontaneous ovulation occurs in 13% of mutant virgin females (virtually no ovulation occurs in wild-type virgin females). The level of ovulation of mutant mated females is not as high as that of wild-type mated females. The mutant females show reduced receptivity after mating (as occurs in wild-type females), but their remating rate 24 hours after mating is significantly higher than that of wild-type females. Receptivity of mutant females is restored earlier than for wild-type females; the remating rate is significantly higher for mutant females compared to wild-type females 10 days after mating. (Ejima et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Kyoto	200524 y¹ w^67c23; P{GSV1}sra^GS3168/TM3, Sb¹ Ser¹
Notes on Origin
Discoverer

Comments
	Excision of the P{GS} element can restore female fertility. (Ejima et al., 2004)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	sra^GS3168 (Takeo et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Takeo et al., 2006, Curr. Biol. 16(14): 1435--1440 The calcineurin regulator Sra plays an essential role in female meiosis in Drosophila. [FBrf0194458] Ejima et al., 2004, Genetics 168(4): 2077--2087 Expression level of sarah, a homolog of DSCR1, is critical for ovulation and female courtship behavior in Drosophila melanogaster. [FBrf0182753]

Allele Dmel\sraGS3168

Allele Dmel\sra^GS3168