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General Information
Symbol
Dmel\spdoUAS.cOa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of O'Conner-Giles
FlyBase ID
FBal0175459
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-spdo
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of the spdo ORF from ESTs RE04681 and RE23355 (2 combined to give one complete ORF because RE23355 contains an apparent missense mutation at aa 289 and RE04681 is incompletely spliced).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of spdoScer\UAS.cOa under the control of Scer\GAL4sca-109-68 induces supernumerary thoracic sensory bristles, as compared to controls.

Expression of spdoScer\UAS.cOa under the control of Scer\GAL4ptc-559.1 promotes ectopic scutellar bristle formation.

Expression of spdoScer\UAS.cOa under the control of Scer\GAL4ptc-559.1 promotes the formation of ectopic sensory organ precursors in late third instar wing imaginal discs.

Expression of spdoScer\UAS.cOa in the wing, under the control of Scer\GAL4nub-AC-62, results in wing notching, vein thickening and reduced wing size.

Expression of spdoScer\UAS.cOa in the embryonic mesoderm, under the control of Scer\GAL4twi.PG, promotes the formation of extra svp-positive heart precursors by stage 12, and extra progenitor svp-positive pericardial cells and cardioblasts by stage 16. Extra svp-negative cardioblasts also develop.

Within somatic clones of Scer\GAL4Act5C.PI cells in a spdoScer\UAS.cOa background, development of notal sensory clusters appears to occur normally in pupae and no defects are seen in notal bristles in adults.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of both spdoScer\UAS.cOa and Rbfox1dsRNA.Scer\UAS under the control of Scer\GAL4sca-109-68 strongly enhances the supernumerary thoracic sensory bristle phenotype observed upon the expression of either spdoScer\UAS.cOa alone or Rbfox1dsRNA.Scer\UAS alone, and even leads to an increase in the number of other (i.e. non-bristle) sensory organs in the thorax, as compared to controls.

Ectopic scutellar bristle formation in flies expressing spdoScer\UAS.cOa under the control of Scer\GAL4ptc-559.1 is dramatically enhanced in a Df(1)N-81k1/+ background.

Ectopic scutellar bristle formation in flies expressing spdoScer\UAS.cOa under the control of Scer\GAL4ptc-559.1 is suppressed by co-expression of numbdsRNA.Scer\UAS.T:Hsap\MYC.

Expression of spdoScer\UAS.cOa under the control of Scer\GAL4nub-AC-62 results in dramatically enhanced wing notching, vein thickening, and reduced wing size in a Df(1)N-81k1/+ background.

Simultaneously expressing spdoScer\UAS.cOa under the control of Scer\GAL4nub-AC-62 in a Df(1)N-81k1/+ and numb2/+ background partially suppresses wing notching, vein thickening, and reduced wing size observed in the absence of numb2.

There is a slightly reduced number of ectopic scutellar bristles in flies expressing spdoScer\UAS.cOa under the control of Scer\GAL4nub-AC-62 in a numb1/+ background.

There is a slightly reduced number of ectopic scutellar bristles in flies expressing spdoScer\UAS.cOa under the control of Scer\GAL4nub-AC-62 in a numb2/+ background.

Expression of spdoScer\UAS.cOa under the control of Scer\GAL4twi.PG in the presence of numb2/+ suppresses the formation of extra svp-positive heart precursors by stage 12, and extra progenitor svp-positive pericardial cells, to almost wildtype levels.

Expression of spdoScer\UAS.cOa under the control of Scer\GAL4twi.PG in a numb2 mutant embryo blocks the formation of svp-positive precursor cells, resulting in dramatically fewer svp-positive heart cells and less cardioblasts. Fewer svp-negative cardioblasts develop.

eve-positive equivalence groups form but less eve-positive pericardial cells are seen compared to wildtype when spdoScer\UAS.cOa is expressed under the control of Scer\GAL4twi.PG in a numb2 background.

eve-positive equivalence groups form normally in numb2 mutant embryos that express spdoScer\UAS.cOa under the control of Scer\GAL4twi.PG. However, eve-positive precursors fail to segregate from approximately half of the equivalence groups, resulting in the formation of far fewer eve-positive pericardial cells than in numb2 mutant embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of spdoScer\UAS.cOa under the control of Scer\GAL4twi.PG in a spdoG104 mutant background rescues the loss of eve-positive pericardial cell numbers observed in spdoG104 mutant embryos.

spdoScer\UAS.cOa; Scer\GAL4sca-109-68 completely or almost completely rescues formation of U2 neurons and suppresses RP2 neuron duplication in spdoG104 homozygous embryos.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
spdoScer\UAS.cOa
spdoUAS.cOa
Name Synonyms
Saccharomyces cerevisiae UAS construct of O'Conner-Giles
Secondary FlyBase IDs
    References (8)