Allele Dmel\nerfin-1¹⁵⁹

General Information
Symbol	Dmel\nerfin-1¹⁵⁹	Species	D. melanogaster
Name		FlyBase ID	FBal0175568
Feature type	allele	Associated gene	Dmel\nerfin-1

Allele class
Mutagen	recombination, FLPase, SCEI endonuclease, ends-in gene targeting
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-in gene targeting (Kuzin et al., 2005) FLPase (Kuzin et al., 2005) recombination (Kuzin et al., 2005) SCEI endonuclease (Kuzin et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Two deletions remove a 1314bp and a 593bp segment that includes the minimal promoter and 5' transcribed leader sequence. (Kuzin et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference nerfin-1¹⁵⁹/nerfin-1[+] is a suppressor \| partially \| somatic clone of neuroanatomy defective \| somatic clone \| adult stage phenotype of pros^IG2227 (Hartl et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference nerfin-1¹⁵⁹/nerfin-1[+] is a suppressor \| partially \| somatic clone of carbon dioxide sensitive neuron \| ectopic \| somatic clone phenotype of pros^IG2227 (Hartl et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference The number of ectopic CO[[2]] neurons on the maxillary palp in animals containing pros[IG2227] clones is significantly suppressed if they are also carrying nerfin-1[159]/+. (Hartl et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Created as a consequence of the "ends in" homologous recombination gene knockout technique. The deletion was most likely caused by exonuclease digestion of the targeting vector after the SceI exonuclease induced double strand break but before its integration into the nerfin-1 chromosomal locus. The deletion was detected after the allelic substitution step and are most likely the result of illegitimate recombination between micro-homologies present in the minimal promoter of one copy of the nerfin-1 duplication and the transcribed region of the tandem copy. (Kuzin et al., 2005)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Df(3L)nerfin-1¹⁵⁹ (Kuzin et al., 2005) nerfin-1¹⁵⁹ nerfin-1^null (Kuzin et al., 2005) nerfin¹⁵⁹ (Hartl et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Hartl et al., 2011, J. Neurosci. 31(44): 15660--15673 A New Prospero and microRNA-279 Pathway Restricts CO2 Receptor Neuron Formation. [FBrf0216631] Kuzin et al., 2005, Dev. Biol. 277(2): 347--365 Nerfin-1 is required for early axon guidance decisions in the developing Drosophila CNS. [FBrf0183075]

Allele Dmel\nerfin-1159

Allele Dmel\nerfin-1¹⁵⁹