FB2025_01 , released February 20, 2025
Allele: Dmel\nejUAS.Tag:V5
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General Information
Symbol
Dmel\nejUAS.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0175571
Feature type
allele
Associated gene
Dmel\nej
Associated Insertion(s)
Carried in Construct
P{UAS-nej.wt-V5}
Also Known As
UAS-CBP FL, UAS-CBP, UAS-nej, UAS-nej.wt-V5
Key Links
Transgenic product class
wild_type
(Ludlam et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Ludlam et al., 2002)
Mutagen
in vitro construct
(Kumar et al., 2004, Ludlam et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-nej.wt-V5}
Cytology
Description

UASt regulatory sequences drive expression of full length, wild-type nej tagged with Tag:V5.

(Ludlam et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
nej
Tagged with
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PU does not cause any obvious eye defects in adults, or defects of axon projections from retinal neurons in third instar eye discs, as compared to wild type.

      (Cutler et al., 2015)

      Expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF at 29[o]C results in a small rough eye phenotype.

      (Tie et al., 2009)

      Third instar larvae expressing nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4Pxn.PS contain an increased proportion of lamellocytes as a proportion of total hemocyte number (5.5% compared to 0.5% in controls). Hemocytes are able to undergo phagocytosis; as in wild type they are able to engulf India ink particles.

      (Stofanko et al., 2008)

      Expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4bbg-C96 results in a wing notching phenotype.

      (Li et al., 2007)

      Overexpression of nejScer\UAS.T:SV5\V5 driven by Scer\GAL4hs.PB does not affect hydroxyurea-mediated cell cycle arrest.

      (Smolik and Jones, 2007)

      Expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF results in severe degeneration of the retina.

      (Anderson et al., 2005)

      Expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF results in adults with a severe rough eye phenotype. Very few, if any, photoreceptor cells are present in newly eclosed adults, although the photoreceptor cells appear to be recruited and specified correctly within the eye imaginal disc in larvae. Cone cell formation during pupal development appears to be abnormal in these animals; the number of cone cells per ommatidium is increased to 5 or 6 cells (compared to 4 in wild type). The severe rough eye phenotype caused by expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF is suppressed if the flies also carry nej3/+.

      (Kumar et al., 2004)

      Flies expressing nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF have smooth eyes which completely lack ommatidia and mechanosensory bristles. Photoreceptors are not detected in these animals.

      (Ludlam et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PF, nejUAS.Tag:V5 has visible phenotype, suppressible by E(z)c.hs

      (Tie et al., 2009)
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      nejUAS.Tag:V5, Scer\GAL4repo.Switch.2301 is a non-enhancer of short lived | RU486 conditional phenotype of Hsap\HTTN548.Q128.UAS, Scer\GAL4repo.Switch.2301

      (Martin et al., 2021)
      Suppressor of
      NOT Suppressor of
      Other
      Statement
      Reference

      Scer\GAL4GMR.PF, mamoMZD.UAS.Tag:FLAG, nejUAS.Tag:V5 has lethal | temperature conditional phenotype

      (Nakamura et al., 2020)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PF, nejUAS.Tag:V5 has eye phenotype, suppressible by E(z)c.hs

      (Tie et al., 2009)
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of E(z)c.hs partially suppresses the mutant eye phenotype caused by expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF at 29[o]C; the eyes are normal in size and the rough eye phenotype is much milder.

      100% of Pc3/+ males that are also expressing nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4hs.PB at 29[o]C have sex comb bristles on the T3 legs.

      (Tie et al., 2009)

      Expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4ey.PH suppresses the no-eye phenotype of soD/+; eye imaginal discs are nearly normal in size and contain large numbers of photoreceptor cell clusters, and in adults, the eyes are fully pigmented, although they are not normally patterned.

      (Kumar et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of nejScer\UAS.T:SV5\V5 partially rescues the small eye and lens fusion phenotype seen in adult flies expressing Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4GMR.PU. Co-expression of nejScer\UAS.T:SV5\V5 partially suppresses the increased cell death and aberrant axonal targeting observed in third instar eye discs of flies expressing Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4GMR.PU.

      (Cutler et al., 2015)
      Complementation and Rescue Data
      Rescues

      nejUAS.Tag:V5/Scer\GAL4hs.PB rescues nejRNAi.UAS.cSa

      (Smolik and Jones, 2007)
      Comments

      Expression of nejScer\UAS.T:SV5\V5 rescues the mitosis phenotypes in eye discs resulting from Scer\GAL4hs.PB-driven nejdsRNA.Scer\UAS expression.

      (Smolik and Jones, 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      nejScer\UAS.T:SV5\V5
      nejUAS.Tag:V5
      Name Synonyms
      Secondary FlyBase IDs
        References (18)