FlyBase Allele Report: Dmel\nej[ΔHQ.UAS]

General Information

Symbol

Dmel\nej^ΔHQ.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0175577

Feature type

allele

Associated gene

Dmel\nej

Associated Insertion(s)

Carried in Construct

P{UAS-nej.ΔHQ}

Key Links

Transgenic product class

inframe_deletion

(Kumar et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Kumar et al., 2004)

Mutagen

in vitro construct

(Kumar et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-nej.ΔHQ}

Cytology

Description

UASt regulatory sequences drive expression of a truncated form of nej (encodes amino acids 1-1998).

(Kumar et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nej

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cone cell | ectopic, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

eye, with Scer\GAL4^ey.PH

(Kumar et al., 2004)

eye, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

ommatidial precursor cluster, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

ommatidium, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R3, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R3 | ectopic, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R4, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R7, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R7 | ectopic, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

Detailed Description

Statement

Reference

Expression of nej^{ΔHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PU does not result in any obvious eye phenotypes in adults.

(Cutler et al., 2015)

Expression of nej^{ΔHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in adults with a moderate rough eye phenotype. Each ommatidium contains a variable number of photoreceptor cells. Ommatidia in the pupal eye disc often contain additional accessory cone cells. Expression of nej^{ΔHQ.Scer\UAS} under the control of Scer\GAL4^hs.2sev results in several defects in ommatidial assembly. Firstly, some ommatidia contain two R3 cells (indicating that a putative R4 cell has instead adopted the R3 fate). Secondly, some ommatidia of opposite chirality from wild type are seen (indicating that the R3 and R4 cells have adopted the opposite cell fates). Thirdly, the R4 cells fails to be specified in some ommatidia. Fourthly, in some ommatidia either the R3 or R4 cell or both has adopted an R7 cell fate. Finally, in rare cases the presumptive R7 cell fails to differentiate. The mild rough eye phenotype caused by expression of nej^{ΔHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not affected if the flies also carry nej³/+.

(Kumar et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

nej^ΔHQ.UAS, Scer\GAL4^GMR.PU is a suppressor of abnormal size | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔHQ.UAS, Scer\GAL4^GMR.PU is a suppressor of increased cell death | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

nej^ΔHQ.UAS, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔHQ.UAS, Scer\GAL4^GMR.PU is a suppressor of lens phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔHQ.UAS, Scer\GAL4^GMR.PU is a suppressor of eye disc | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of nej^{ΔHQ.Scer\UAS} partially rescues the small eye and lens fusion phenotype seen in adult flies expressing Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU. Co-expression of nej^{ΔHQ.Scer\UAS} partially suppresses the increased cell death observed in third instar eye discs of flies expressing Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU.

(Cutler et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nej^{ΔHQ.Scer\UAS}

nej^ΔHQ.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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