Allele Dmel\bsk^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\bsk^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0175755
Feature type	allele	Associated gene	Dmel\bsk

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Ishimaru et al., 2004, Ishimaru et al., 2004) in vitro construct - RNAi (Ishimaru et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression bsk coding sequences (from GenBank: nucleotides 536 to 1260) repeated in dyad symmetry. (Ishimaru et al., 2004)
Carried in construct	P{UAS-bsk.IR} (Ishimaru et al., 2004, Ishimaru et al., 2004, Igaki et al., 2006, Neisch et al., 2010, Bidla et al., 2007, Ohsawa et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	embryonic/larval hemolymph \| third instar larval stage, with Scer\GAL4^Act5C.PU (Bidla et al., 2007) embryonic/larval hemolymph \| third instar larval stage, with Scer\GAL4^lz-gal4 (Bidla et al., 2007) thorax, with Scer\GAL4^pnr-MD237 (Ishimaru et al., 2004)
Detailed Description
	Statement Reference Hemolymph clots from third instar larvae expressing bsk[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.PU] show a complete lack of melanization. Hemolymph clots from third instar larvae expressing bsk[dsRNA.Scer\UAS] in crystal cells under the control of Scer\GAL4[lz-gal4] show a reduction in melanization. (Bidla et al., 2007) When bsk^{dsRNA.Scer\UAS} is driven by Scer\GAL4^pnr-MD237, thorax closure in the adult fly is defective. (Ishimaru et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Expression of bsk[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act.PU] in the cells surrounding scrib[1] clones in the eye-antennal disc significantly suppresses the elimination of the mutant scrib[1] clones compared to that seen when the cells surrounding the clone are wild type. (Ohsawa et al., 2011) Co-expression of bsk[dsRNA.Scer\UAS] with hep[CA.Scer\UAS] in eye clones, under the control of Scer\GAL4[GMR.PF] results in partial suppression of the apoptotic eye phenotype observed in hep[CA.Scer\UAS] mutant clones. (Igaki et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	bsk^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Ohsawa et al., 2011, Dev. Cell 20(3): 315--328 Elimination of Oncogenic Neighbors by JNK-Mediated Engulfment in Drosophila. [FBrf0213219] Neisch et al., 2010, J. Cell Biol. 189(2): 311--323 Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. [FBrf0210554] Bidla et al., 2007, J. Cell Sci. 120(7): 1209--1215 Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog Eiger. [FBrf0192653] Igaki et al., 2006, Curr. Biol. 16(11): 1139--1146 Loss of cell polarity drives tumor growth and invasion through JNK activation in Drosophila. [FBrf0194200] Ishimaru et al., 2004, EMBO J. 23(20): 3984--3994 PVR plays a critical role via JNK activation in thorax closure during Drosophila metamorphosis. [FBrf0180198]
Supplementary material	Ishimaru et al., 2004, EMBO J. 23(20): Supplementary data. [FBrf0182966]

Allele Dmel\bskdsRNA.Scer\UAS

Allele Dmel\bsk^{dsRNA.Scer\UAS}