Allele Dmel\VGlut^Scer\UAS.cDa

General Information
Symbol	Dmel\VGlut^Scer\UAS.cDa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Daniels	FlyBase ID	FBal0175767
Feature type	allele	Associated gene	Dmel\VGlut

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Daniels et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a VGlut cDNA. (Daniels et al., 2004)
Carried in construct	P{UAS-VGlut.D} (Daniels et al., 2004, Grosjean et al., 2008, Daniels et al., 2011, Daniels et al., 2006, Marie et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	courtship behavior defective \| male, with Scer\GAL4^NP0225 (Grosjean et al., 2008) courtship behavior defective \| male, with Scer\GAL4^αTub84B.PL (Grosjean et al., 2008) flightless, with Scer\GAL4^VGlut.PD (Daniels et al., 2011) gravitaxis defective, with Scer\GAL4^VGlut.PD (Daniels et al., 2011) lethal, with Scer\GAL4^VGlut.PD (Daniels et al., 2011) lethal \| larval stage, with Scer\GAL4^BG380 (Daniels et al., 2011) neuroanatomy defective, with Scer\GAL4^BG380 (Daniels et al., 2004) neurophysiology defective, with Scer\GAL4^BG380 (Daniels et al., 2004, Daniels et al., 2011) neurophysiology defective, with Scer\GAL4^elav-C155 (Marie et al., 2010) neurophysiology defective, with Scer\GAL4^VGlut.PD (Daniels et al., 2011) uncoordinated, with Scer\GAL4^VGlut.PD (Daniels et al., 2011)
Phenotype Manifest In
	embryonic/larval neuromuscular junction, with Scer\GAL4^BG380 (Daniels et al., 2004) neuromuscular junction, with Scer\GAL4^elav-C155 (Marie et al., 2010) synaptic vesicle, with Scer\GAL4^BG380 (Daniels et al., 2004)
Detailed Description
	Statement Reference Intracellular voltage recordings in muscles of animals overexpressing VGlut[Scer\UAS.cDa] driven by Scer\GAL4[VGlut.PD] or Scer\GAL4[BG380] reveal large spontaneous events in motoneurons. Driving VGlut[Scer\UAS.cDa] withe the strong Scer\GAL4[BG380] driver results in death during larval stages. Driving VGlut[Scer\UAS.cDa] with Scer\GAL4[VGlut.PD] results in lethality within the first few weeks of adulthood. Flies overexpressing VGlut[Scer\UAS.cDa] driven by Scer\GAL4[VGlut.PD] are uncoordinated and cannot fly. VGlut[Scer\UAS.cDa]-overexpressing flies display progressive motor defects manifesting in impaired negative geotaxis. (Daniels et al., 2011) Expression of VGlut[Scer\UAS.cDa] in post-mitotic neurons under the control of Scer\GAL4[elav-C155] causes a significant increase in average mEPSP amplitude. There is also a homeostatic decrease in presynaptic quantal content in these mutants. (Marie et al., 2010) Males expressing VGlut^Scer\UAS.cDa under the control of Scer\GAL4^αTub84B.PL show high levels of homosexual courtship, which includes all aspects of sexual behaviour (including singing, genital licking and attempted copulation). Occasionally, the males attempt copulation with inappropriate body reions, such as the head. Males expressing VGlut^Scer\UAS.cDa under the control of Scer\GAL4^NP0225 show a significant amount of homosexual behaviour, although less than seen when the Scer\GAL4^αTub84B.PL driver is used. Expression of VGlut^Scer\UAS.cDa under the control of Scer\GAL4^Mef2.247 has no affect on the courtship behaviour of males. (Grosjean et al., 2008) Expression of VGlut^Scer\UAS.cDa under the control of Scer\GAL4^BG380 results in an increase of about 60% in the mean mEJP amplitude recorded from the muscle of the larval neuromuscular junction compared to wild type. The amplitude of the entire population of mEJP events seems to be increased in size, with the median amplitude increasing by 35% compared to wild type. There is a small increase in mEJP frequency. Larvae expressing VGlut^Scer\UAS.cDa under the control of Scer\GAL4^BG380 show no significant difference in the amplitude of evoked EJPs recorded from the muscle of the larval neuromuscular junction compared to wild type. Quantal content is decreased relative to wild type. Larvae expressing VGlut^Scer\UAS.cDa under the control of Scer\GAL4^BG380 show an increase in synaptic vesicle size (assayed at the neuromuscular junction of the type Ib motorneuron that innervates muscles 6 and 7) compared to wild type; a 14% increase in mean vesicle outer diameter and a median increase in vesicle outer diameter of 10% is seen. (Daniels et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^elav-C155, VGlut^Scer\UAS.cDa has neurophysiology defective phenotype, non-enhanceable by Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} (Marie et al., 2010)
NOT suppressed by
Statement Reference Scer\GAL4^elav-C155, VGlut^Scer\UAS.cDa has neurophysiology defective phenotype, non-suppressible by Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} (Marie et al., 2010)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Scer\GAL4^elav-C155, VGlut^Scer\UAS.cDa has neuromuscular junction phenotype, non-enhanceable by Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} (Marie et al., 2010)
NOT suppressed by
Statement Reference Scer\GAL4^elav-C155, VGlut^Scer\UAS.cDa has neuromuscular junction phenotype, non-suppressible by Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} (Marie et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Expression of VGlut[Scer\UAS.cDa] and gsb[491.dsRNA.Scer\UAS] in post-mitotic neurons under the control of Scer\GAL4[elav-C155] causes a significant increase in average mEPSP amplitude. There is also a homeostatic decrease in presynaptic quantal content in these mutants. (Marie et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^BG380/VGlut^Scer\UAS.cDa rescues Df(2L)VGlut²/VGlut¹ (Daniels et al., 2006)
Comments	Expression of VGlut[Scer\UAS.cDa] in motor neurons under the control of Scer\GAL4[BG380] rescues the decrease in mEJP frequency in VGlut[1]/Df(2L)VGlut[2] mutant synapses. These larvae exhibit a slight increase in quantal size compared to controls. (Daniels et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	VGlut^Scer\UAS.cDa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Daniels (Daniels et al., 2004)
Secondary FlyBase IDs

References ( 5 )
Research paper	Daniels et al., 2011, Neurobiol. Disease 41(2): 415--420 Increased vesicular glutamate transporter expression causes excitotoxic neurodegeneration. [FBrf0212635] Marie et al., 2010, J. Neurosci. 30(24): 8071--8082 Synaptic homeostasis is consolidated by the cell fate gene gooseberry, a Drosophila pax3/7 homolog. [FBrf0211066] Grosjean et al., 2008, Nat. Neurosci. 11(1): 54--61 A glial amino-acid transporter controls synapse strength and courtship in Drosophila. [FBrf0201004] Daniels et al., 2006, Neuron 49(1): 11--16 A single vesicular glutamate transporter is sufficient to fill a synaptic vesicle. [FBrf0191312] Daniels et al., 2004, J. Neurosci. 24(46): 10466--10474 Increased expression of the Drosophila vesicular glutamate transporter leads to excess glutamate release and a compensatory decrease in quantal content. [FBrf0180418]

Allele Dmel\VGlutScer\UAS.cDa

Allele Dmel\VGlut^Scer\UAS.cDa