Imprecise excision of the P{EP} element, resulting in a deletion extending from -40 to +2330bp (relative to the translation start site).
Lig4169 mutants display mildly increased frequency of egg shell defects compared to wild-type controls.
The proportion of homologous recombination during zinc-finger induced gene targeting is dramatically increased at the expense of non-homologous end joining in homozygous Lig4169 mutants.
Female homozygous and male hemizygous third instar larvae do not show hypersensitivity to gamma irradiation. Homozygotes are not hypersensitive to methyl methanesulfonate. Excision and repair events of P{hswa} in Lig4169 males carrying P{hswa} and a source of transposase have been studied. These studies show that the mutant males are not impaired in their ability to repair P-element-induced double-strand breaks through incomplete synthesis-dependent strand annealing (SDSA) and, in addition, complete SDSA appears to proceed normally in these flies.
DNAlig4169/DNAlig4169 is an enhancer of majority die during embryonic stage phenotype of PolQnull
DNAlig4169/DNAlig4169 is an enhancer of short lived phenotype of BlmN1
DNAlig4169 is an enhancer of radiation sensitive phenotype of spn-A093A/spn-A3
DNAlig4169/DNAlig4169 is a non-enhancer of tumorigenic phenotype of BlmN1
DNAlig4169, spn-A093A/spn-A3 has viable phenotype
DNAlig4169/DNAlig4169 is an enhancer of egg phenotype of PolQnull
DNAlig4169/DNAlig4169 is a non-enhancer of adult gut phenotype of BlmN1
DNAlig4169/DNAlig4169 is a non-enhancer of testis | adult stage phenotype of BlmN1
DNAlig4169/DNAlig4169 is a non-enhancer of ovary | adult stage phenotype of BlmN1
The high frequency of eggshell defects characteristic for mus308null mutants is increased further by combination with Lig4169 and the mus308null;Lig4169 double mutants fail to hatch.
There is a further decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hswa} in Lig4169 ; mus308ZIII-2003/Df(3R)kar3l double mutant males compared to the percentage seen in mus308ZIII-2003/Df(3R)kar3l single mutant males. 100% of the end-joining repair products are associated with large deletions in the double mutant males.
DNAlig4169 homozygosity does not significantly affect the enlarged wing disc posterior compartment nor the DNA damage response kinetics induced upon exposure to ionizing radiation of individuals expressing BacA\p35UAS.cHa under the control of Scer\GAL4en.PU.
DNAlig4169 is rescued by DNAlig4+t6