FlyBase Allele Report: Dmel\DNAlig4[169]

General Information

Symbol

Dmel\DNAlig4¹⁶⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0176089

Feature type

allele

Associated gene

Dmel\DNAlig4

Associated Insertion(s)

Carried in Construct

Also Known As

lig4¹⁶⁹, lig4^169a

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(McVey et al., 2004)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

DNAlig4^EP385

(McVey et al., 2004)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of the P{EP} element, resulting in a deletion extending from -40 to +2330bp (relative to the translation start site).

(McVey et al., 2004)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg

(Alexander et al., 2016)

Detailed Description

Statement

Reference

Lig4¹⁶⁹ mutants display mildly increased frequency of egg shell defects compared to wild-type controls.

(Alexander et al., 2016)

Lig4¹⁶⁹/Lig4¹⁶⁹ mutant adults do not exhibit a significant decrease in lifespan nor a significant increase in tumor formation, as compared to wild type.

(Garcia et al., 2011)

The frequency of insertions at repair junctions during alternative end-joining after Scer\SCEI-induced double-strand breaks is significantly increased relative to wild-type in Lig4¹⁶⁹ mutants.

(Yu and McVey, 2010)

The proportion of homologous recombination during zinc-finger induced gene targeting is dramatically increased at the expense of non-homologous end joining in homozygous Lig4¹⁶⁹ mutants.

(Beumer et al., 2008)

Female homozygous and male hemizygous third instar larvae do not show hypersensitivity to gamma irradiation. Homozygotes are not hypersensitive to methyl methanesulfonate. Excision and repair events of P{hsw^a} in Lig4¹⁶⁹ males carrying P{hsw^a} and a source of transposase have been studied. These studies show that the mutant males are not impaired in their ability to repair P-element-induced double-strand breaks through incomplete synthesis-dependent strand annealing (SDSA) and, in addition, complete SDSA appears to proceed normally in these flies.

(McVey et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

DNAlig4¹⁶⁹/DNAlig4¹⁶⁹ is an enhancer of majority die during embryonic stage phenotype of PolQ^null

(Alexander et al., 2016)

DNAlig4¹⁶⁹/DNAlig4¹⁶⁹ is an enhancer of short lived phenotype of Blm^N1

(Garcia et al., 2011)

DNAlig4¹⁶⁹ is an enhancer of radiation sensitive phenotype of spn-A^093A/spn-A³

(McVey et al., 2004)

NOT Enhancer of

Statement

Reference

DNAlig4¹⁶⁹/DNAlig4¹⁶⁹ is a non-enhancer of neoplasia phenotype of Blm^N1

(Garcia et al., 2011)

Other

Statement

Reference

DNAlig4¹⁶⁹, spn-A^093A/spn-A³ has viable phenotype

(McVey et al., 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

DNAlig4¹⁶⁹/DNAlig4¹⁶⁹ is an enhancer of egg phenotype of PolQ^null

(Alexander et al., 2016)

NOT Enhancer of

Statement

Reference

DNAlig4¹⁶⁹/DNAlig4¹⁶⁹ is a non-enhancer of adult gut phenotype of Blm^N1

(Garcia et al., 2011)

DNAlig4¹⁶⁹/DNAlig4¹⁶⁹ is a non-enhancer of testis | adult stage phenotype of Blm^N1

(Garcia et al., 2011)

DNAlig4¹⁶⁹/DNAlig4¹⁶⁹ is a non-enhancer of ovary | adult stage phenotype of Blm^N1

(Garcia et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The high frequency of eggshell defects characteristic for mus308^null mutants is increased further by combination with Lig4¹⁶⁹ and the mus308^null;Lig4¹⁶⁹ double mutants fail to hatch.

(Alexander et al., 2016)

The presence of Lig4¹⁶⁹/Lig4¹⁶⁹ does not significantly enhance the severity of tumor formation, but does enhance the decreased lifespan seen in Blm^N1/Blm^N1 mutant adults.

(Garcia et al., 2011)

There is a further decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hsw^a} in Lig4¹⁶⁹ ; mus308^ZIII-2003/Df(3R)kar3l double mutant males compared to the percentage seen in mus308^ZIII-2003/Df(3R)kar3l single mutant males. 100% of the end-joining repair products are associated with large deletions in the double mutant males.

(Chan et al., 2010)

At intermediate doses (400-500 rad) of gamma irradiation, Lig4¹⁶⁹ spn-A³/spn-A^093A double mutant larvae show increased sensitivity to irradiation compared to spn-A³/spn-A^093A single mutants.

(McVey et al., 2004)

Xenogenetic Interactions

Statement

Reference

DNAlig4¹⁶⁹ homozygosity does not significantly affect the enlarged wing disc posterior compartment nor the DNA damage response kinetics induced upon exposure to ionizing radiation of individuals expressing BacA\p35^UAS.cHa under the control of Scer\GAL4^en.PU.

(Dekanty et al., 2015)

Complementation and Rescue Data

Rescued by

DNAlig4¹⁶⁹ is rescued by DNAlig4^+t6

(White and Lambowitz, 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

28877