Allele Dmel\Kap-α3^{-UTR.Scer\UAS.P\T}

General Information
Symbol	Dmel\Kap-α3^{-UTR.Scer\UAS.P\T}	Species	D. melanogaster
Name		FlyBase ID	FBal0176093
Feature type	allele	Associated gene	Dmel\Kap-α3

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Mason et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of the Kap-α3 ORF (no UTRs) is under the control of Scer\UAS and P\T promoter sequences. (Mason et al., 2003)
Carried in construct	P{UASp-Kap-α3.-UTR} (Mason et al., 2003, Ratan et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement Reference Scer\GAL4^{nos.UTR.T:Hsim\VP16}/Kap-α3^{-UTR.Scer\UAS.P\T} is a non-suppressor of nurse cell ring canal phenotype of Pen^D14 (Mason et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference Expression of Kap-α3[-UTR.Scer\UAS.P\T] under the control of Scer\GAL4[Act5C.PI] does not restore fertility in Df(3L)α1S1 males. Expression of Kap-α3[-UTR.Scer\UAS.P\T] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] does not restore fertility or fecundity in Df(3L)α1S1 females. (Ratan et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^αTub84B.PL/Kap-α3^{-UTR.Scer\UAS.P\T} rescues Kap-α3^17-7/Kap-α3^D93 (Mason et al., 2003) Scer\GAL4^αTub84B.PL/Kap-α3^{-UTR.Scer\UAS.P\T} rescues Kap-α3^w73/Kap-α3^D93 (Mason et al., 2003)
Partially rescues	Scer\GAL4^ey.PH/Kap-α3^{-UTR.Scer\UAS.P\T} partially rescues Kap-α3^D93 (Mason et al., 2003) Scer\GAL4^αTub84B.PL/Kap-α3^{-UTR.Scer\UAS.P\T} partially rescues Df(3R)GB104/Kap-α3^D93 (Mason et al., 2003) Scer\GAL4^αTub84B.PL/Kap-α3^{-UTR.Scer\UAS.P\T} partially rescues Kap-α3^D93 (Mason et al., 2003) Scer\GAL4^αTub84B.PL/Kap-α3^{-UTR.Scer\UAS.P\T} partially rescues Kap-α3^D93/Kap-α3^D165 (Mason et al., 2003)
Comments	Kap-α3^{-UTR.Scer\UAS.P\T}; Scer\GAL4^αTub84B.PL weakly rescues the larval lethality of Kap-α3^D93/Kap-α3^D93, Kap-α3^D93/Kap-α3^D165 (around 40% still die as larvae, but about 50% die as pupae and about 10% as pharate adults) and Kap-α3^D93/Df(3R)GB104 (about 30% survive to pupate before dying). Kap-α3^{-UTR.Scer\UAS.P\T}; Scer\GAL4^αTub84B.PL rescues Kap-α3^D93/Kap-α3^17-7 and Kap-α3^D93/Kap-α3^w73 to fully viable, fertile adults. Kap-α3^{-UTR.Scer\UAS.P\T}; Scer\GAL4^ey.PH partially rescues the eye phenotypes due to Kap-α3^D93 somatic clones: Externally, individual ommatidia are much more distinct than without rescue, but most interommatidial bristles are missing. Sectioning reveals ommatidia to be somewhat disorganised and misshapen, with incorrect numbers of photoreceptors per ommatidium. Unlike in the non-rescued animals, rhabdomeres are present. (Mason et al., 2003)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Kap-α3^{-UTR.Scer\UAS.P\T}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Ratan et al., 2008, Genetics 178(2): 839--850 Drosophila importin alpha1 performs paralog-specific functions essential for gametogenesis. [FBrf0204254] Mason et al., 2003, Genetics 165(4): 1943--1958 The Drosophila melanogaster importin alpha3 locus encodes an essential gene required for the development of both larval and adult tissues. [FBrf0167653]

Allele Dmel\Kap-α3-UTR.Scer\UAS.P\T

Allele Dmel\Kap-α3^{-UTR.Scer\UAS.P\T}