Construct contains two overlapping regions of Hr78 separated by a 100 bp spacer; one region is an inverted repeat of the last 1004 bp of the Hr78 gene. The Hr78 sequences are expressed under the control of UASt regulatory sequences.
embryonic/larval trachea, with Scer\GAL4btl.PS
embryonic/larval trachea, with Scer\GAL4da.G32
Expression of Hr78dsRNA.Scer\UAS driven by the constitutively expressed Scer\GAL4da.G32 results in 81% of larvae examined displaying tracheal defects, including necrosis. The same phenotype is seen when Hr78dsRNA.Scer\UAS expression is driven by Scer\GAL4btl.PS, a tracheal specific driver, although only 13% of larvae are affected. Expression of Hr78dsRNA.Scer\UAS driven by Scer\GAL4Dll-md23 does not result in tracheal defects.
Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Hr78 gene.