Allele Dmel\Ext2^{Act5C.T:SV5\V5}

General Information
Symbol	Dmel\Ext2^{Act5C.T:SV5\V5}	Species	D. melanogaster
Name		FlyBase ID	FBal0176153
Feature type	allele	Associated gene	Dmel\Ext2

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Han et al., 2004) in vitro construct - regulatory fusion (Han et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Act5C promoter sequences drive expression of Ext2 tagged with T:SV5\V5. (Belenkaya et al., 2008) Construct: Expression of the coding region of the Ext2 cDNA fused to the epitope tag T:SV5\V5, is under the control of Scer\UAS sequences. (Han et al., 2004)
Carried in construct	P{Ac5.1-sotv.V5} (Belenkaya et al., 2008)
Tagged with	T:SV5\V5 (Han et al., 2004, Belenkaya et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in a plamsid, transfected into S2 cells to study protein-protein interaction. (Han et al., 2004)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Ext2^{Act5C.T:SV5\V5}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Belenkaya et al., 2008, Dev. Cell 14(1): 120--131 The retromer complex influences Wnt secretion by recycling Wntless from endosomes to the trans-Golgi network. [FBrf0203062] Han et al., 2004, Development 131(7): 1563--1575 Distinct and collaborative roles of Drosophila EXT family proteins in morphogen signalling and gradient formation. [FBrf0174560]

Allele Dmel\Ext2Act5C.T:SV5\V5

Allele Dmel\Ext2^{Act5C.T:SV5\V5}