Open Close
General Information
Symbol
Dmel\Drp11
Species
D. melanogaster
Name
FlyBase ID
FBal0176235
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Mutation identified by sequence analysis.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Drp11 homozygous mutant MARCM clones of glutamatergic neurons in the proximal wing have significantly increased mitochondrial length in the axons and mitochondrial hyperfusion in the cell bodies, compared to controls.

    Drp11/Drp12 transheterozygote mutants are lethal.

    Drp11/Drp12 flies do not show clumping of mitochondria or muscle cell death in the indirect flight muscles.

    In Drp11/Drp12 mutant adult flight muscles, the mitochondria are enlarged (being round in shape) and fewer in number compared to wild type but contain intact internal structures.

    Drp11/Drp12 cells contain fewer mitochondria per cell than wild type and mitochondrial morphology is abnormal.

    Shows defects in neurotransmitter release.

    Homozygous third instar salivary gland cells show clustering of mitochondria.

    Mitochondrial number per bouton is reduced at Drp11/Drp12 neuromuscular junctions.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Suppressed by
    NOT suppressed by
    Statement
    Reference
    NOT Suppressor of
    Other
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The reduced connectivity of the mitochondrial network in body wall muscles characteristic for third instar larvae expressing maskHMS01045 under the control of Scer\GAL4how-24B cannot is not changed by combination with Drp11/Drp12.

    Expression of either MarfmiRNA.CDS.Scer\UAS, MarfmiRNA.UTR.Scer\UAS or opa1-likemiRNA.CDS.Scer\UAS under the control of Scer\GAL4elav.PU is sufficient to restore a normal filamentous mitochondrial network in Drp11/Drp12 flies.

    Expression of PmiScer\UAS.cRa under the control of Scer\GAL4elav.PU does not rescue mitochondrial morphology in Drp11/Drp12 flies.

    Xenogenetic Interactions
    Statement
    Reference

    The lethality of Drp11/Drp12 transheterozygotes is rescued by expression of either Hsap\DNM1LScer\UAS.cCa or Hsap\DNM1LE379K.Scer\UAS under the control of Scer\GAL4da.PU in the mutant background.

    The lethality of Drp11/Drp12 transheterozygotes is not rescued by expression of either Hsap\DNM1LA395D.Scer\UAS or Hsap\DNM1LG350R.Scer\UAS under the control of Scer\GAL4da.PU in the mutant background.

    Expression of either Hsap\DNM1LScer\UAS.cCa or Hsap\DNM1LE379K.Scer\UAS under the control of either Scer\GAL4Act5C.PI or Scer\GAL4Mef2.PU does not cause lethality when expressed in sensitised Drp11/+ background.

    Expression of Hsap\DNM1LA395D.Scer\UAS under the control of either Scer\GAL4Act5C.PI or Scer\GAL4Mef2.PU causes lethality when expressed in sensitised Drp11/+ background. Expression of Hsap\DNM1LG350R.Scer\UAS causes lethality only when driven by Scer\GAL4Act5C.PI but not when driven by Scer\GAL4Mef2.PU.

    Expression of Hsap\DNM1LScer\UAS.cCa under the control of Scer\GAL4Mef2.PU does not cause any defects in the morphology or number of mitochondria in third instar larval muscles when expressed in sensitised Drp11/+ background. Expression under the control of Scer\GAL4Toll-6-D42 also does not cause any mitochondria trafficking defects in the ventral nerve cord, axons and synaptic boutons (though number of mitochondria per bouton is slightly lower compared to controls) in third instar larvae.

    Expression of Hsap\DNM1LA395D.Scer\UAS under the control of Scer\GAL4Mef2.PU in sensitised Drp11/+ background causes mitochondria morphological defects (large blocks of interconnected mitochondria) as well as defects in their distribution, leading to decreased number of mitochondria per sarcomere or per muscle area in third instar larval muscles. Expression under the control of Scer\GAL4Toll-6-D42 leads to mitochondria trafficking defects in the ventral nerve cord, axons and synaptic boutons in third instar larvae. The number of mitochondria per axon area or per bouton is significantly decreased compared to controls.

    Expression of Hsap\DNM1LG350R.Scer\UAS under the control of Scer\GAL4Mef2.PU in sensitised Drp11/+ background causes mitochondria morphological defects (large blocks of interconnected mitochondria) as well as defects in their distribution, leading to decreased number of mitochondria per sarcomere or per muscle area in third instar larval muscles. Expression under the control of Scer\GAL4Toll-6-D42 also leads to mitochondria trafficking defects in the ventral nerve cord, axons and synaptic boutons in third instar larvae. The number of mitochondria per axon area or per bouton is significantly decreased compared to controls.

    Expression of Hsap\DNM1LE379K.Scer\UAS under the control of Scer\GAL4Mef2.PU in sensitised Drp11/+ background does not cause any defects in the morphology or number of mitochondria in third instar larval muscles. Expression under the control of Scer\GAL4Toll-6-D42 also does not cause mitochondria trafficking defects in the ventral nerve cord and axons but leads to clear trafficking defect at the level of synaptic boutons (number of mitochondria per bouton is significantly decreased compared to controls) in third instar larvae.

    Complementation and Rescue Data
    Comments

    The lethality of Drp11/Drp12 transheterozygotes is rescued by expression of Drp1Scer\UAS.cUa under the control of Scer\GAL4da.PU in the mutant background.

    Expression of Drp1Scer\UAS.cDa under the control of Scer\GAL4elav.PU rescues mitochondrial morphology in Drp11/Drp12 flies.

    Lethality of Drp11/Drp12 rescued by a genomic rescue construct spanning the 'CG3210' annotation.

    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    References (13)