Allele Dmel\Csk^j1D8

General Information
Symbol	Dmel\Csk^j1D8	Species	D. melanogaster
Name	l(3)j1D8^j1D8	FlyBase ID	FBal0176300
Feature type	allele	Associated gene	Dmel\Csk
Also Known As	dCsk^j1D8, l(3)j1D8

Map ( GBrowse )	Untitled Document
Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Stewart et al., 2003, Read et al., 2004)
Mutagen	P-element activity (BDGP Project Members, 1994-1999, Stewart et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:7,472,771..7,472,771 (Stewart et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999, Kusano et al., 2001, Saja et al., 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Consists of 12kb insertion of a FB element, and a crippled P{lacW} element. The insertion also marks the site of a 1.5kb genomic deletion. (Stewart et al., 2003)
Caused by insertion	FB{}Csk^j1D8 (Stewart et al., 2003) P{3'lacW}Csk^j1D8 (Stewart et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999, Kusano et al., 2001, Saja et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death \| recessive \| third instar larval stage (Langton et al., 2007) increased cell number \| somatic clone (Read et al., 2004) large body (Read et al., 2004) large body (with Csk^S030003) (Read et al., 2004) lethal \| pupal stage (with Csk^S030003) (Read et al., 2004) lethal \| pupal stage \| recessive (Read et al., 2004, Langton et al., 2007) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999) mitotic cell cycle defective (with Csk^S030003) (Read et al., 2004) mitotic cell cycle defective \| somatic clone (Read et al., 2004)
Phenotype Manifest In
	eye \| somatic clone (Read et al., 2004) eye-antennal disc \| somatic clone (Read et al., 2004) ommatidium \| somatic clone \| supernumerary (Read et al., 2004) wing disc (Langton et al., 2007)
Detailed Description
	Statement Reference Wing discs of homozygous third instar larvae show considerable apoptosis. (Langton et al., 2007) Mutant larvae die within 6-8 hours after pupariation. The larval body mass of mutants increases up to 180% of the wild-type mass during the wandering stage. The brain ventral ganglion and salivary glands of mutants are enlarged. Pupariation is delayed. The body length of mutant pupae are about 121% the size of wild-type. Imaginal discs of mutant larvae are also larger than wild-type. The larval body mass of Csk^j1D8/Csk^S030003 mutants increases up to 150% of the wild-type mass during the wandering stage. In the rare cases mutants survived to pharate adult stage, the head and eyes are enlarged. The posterior ommatidia are sometimes misaligned, though individual ommatidia are morphologically normal. Mutant eyes contain more ommatidia than wild-type. Rarely the eyes are replaced with duplicated antennae. In addition the wings and legs are severely malformed, the notum is sometimes split, and the head, legs and notum often contain cuticle overgrowths. In Csk^j1D8/Csk^S030003 and homozygous Csk^j1D8 mutant developing eye discs, an increase is seen in the number of mitotic cells in the morphogenetic furrow. Mutant pupal retinas contain excess mitotic cells in the interommatidial lattice. Csk^j1D8/Csk^S030003 eye antennal disc cells exhibit a decrease in the proportion of cells in G₀-G₁ phase, and a consequent increase in cells in G₂-M phase when compared to wild-type controls. Homozygous mutant clones in the eye produced severely overgrown eyes. Eyes have more than 1200 ommatidia, compared to about 687 in wild-type. Eyes can become malformed as a result. The extra ommatidia are occasionally inverted in their planar polarity but are otherwise normal in size and morphology. Occasionally mutant clones result in antennal duplication and cuticle overgrowth. (Read et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Csk^j1D8, puc^E69/puc[+] has visible phenotype, enhanceable by ASPP[+]/ASPP⁸ (Langton et al., 2007)
Suppressed by
Statement Reference ASPP⁸, Csk^j1D8/Csk[+] has visible phenotype, suppressible by Btk29A[+]/Btk29A^k00206 (Langton et al., 2007) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, suppressible \| partially by Src42A^18-2 (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, suppressible by bsk¹ (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, suppressible by Btk29A^k00206 (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, suppressible by Src64B^PI (Read et al., 2004) Csk^j1D8/Csk^S030003 has mitotic cell cycle defective phenotype, suppressible by bsk¹ (Read et al., 2004) Csk^j1D8/Csk^S030003 has mitotic cell cycle defective phenotype, suppressible by Btk29A^k00206 (Read et al., 2004)
NOT suppressed by
Statement Reference Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by CadN^M12 (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by chico¹ (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by Egfr^f2 (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by ft^G-rv (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by Jra^IA109 (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by scb² (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by shg^k03401 (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by Sos^34Ea-6 (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by Src42A^k10108 (Read et al., 2004) Csk^j1D8/Csk^S030003 has lethal \| pupal stage phenotype, non-suppressible by Src42A^Su(phl)1-1 (Read et al., 2004)
Enhancer of
Statement Reference Csk^j1D8/Csk[+] is an enhancer of neuroanatomy defective phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Saja et al., 2010) Csk^j1D8/Csk[+] is an enhancer of visible phenotype of Ret^GMR.PR (Read et al., 2005) Csk^j1D8/Csk[+] is an enhancer of visible phenotype of Ret^MEN2B.GMR (Read et al., 2005) Csk^j1D8 is an enhancer of increased cell number phenotype of os^GMR.PB (Read et al., 2004)
NOT Enhancer of
Statement Reference Csk^j1D8/Csk[+] is a non-enhancer of visible \| dominant phenotype of Egfr^E1 (Read et al., 2005) Csk^j1D8/Csk[+] is a non-enhancer of visible phenotype of Ret^MEN2A.GMR (Read et al., 2005)
Suppressor of
Statement Reference Csk^j1D8/Csk[+] is a suppressor of visible phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^{i480.Scer\UAS.cGa} (Garcia-Lopez et al., 2008)
NOT Suppressor of
Statement Reference Csk^j1D8/Csk[+] is a non-suppressor of visible \| dominant phenotype of Egfr^E1 (Read et al., 2005) Csk^j1D8/Csk[+] is a non-suppressor of visible phenotype of Ret^MEN2A.GMR (Read et al., 2005)
Other
Statement Reference ASPP[+]/ASPP⁸, Csk^j1D8, puc^E69/puc[+] has visible phenotype (Langton et al., 2007) ASPP⁸, Csk^j1D8/Csk[+] has increased cell death \| third instar larval stage phenotype (Langton et al., 2007) ASPP⁸, Csk^j1D8/Csk[+] has visible phenotype (Langton et al., 2007) ASPP^d, Csk^j1D8 has visible phenotype (Langton et al., 2009) Csk^j1D8, puc^E69/puc[+] has increased cell death phenotype (Langton et al., 2007) Csk^j1D8, puc^E69/puc[+] has visible phenotype (Langton et al., 2007) Csk^j1D8, Stat92E⁰⁶³⁴⁶ has increased cell death phenotype (Read et al., 2004, Read et al., 2004)
Phenotype Manifest In
Enhanced by
Statement Reference Csk^j1D8, puc^E69/puc[+] has wing phenotype, enhanceable by ASPP[+]/ASPP⁸ (Langton et al., 2007) Csk^j1D8 has eye \| somatic clone phenotype, enhanceable by Stat92E^j6C8 (Read et al., 2004) Csk^j1D8 has eye-antennal disc \| somatic clone phenotype, enhanceable by Stat92E⁰⁶³⁴⁶ (Read et al., 2004)
Suppressed by
Statement Reference ASPP⁸, Csk^j1D8/Csk[+] has wing disc phenotype, suppressible by Btk29A[+]/Btk29A^k00206 (Langton et al., 2007) ASPP⁸, Csk^j1D8/Csk[+] has wing phenotype, suppressible by Btk29A[+]/Btk29A^k00206 (Langton et al., 2007) Csk^j1D8, Stat92E^j6C8 has eye \| somatic clone phenotype, suppressible by Btk29A^k00206 (Read et al., 2004)
Enhancer of
Statement Reference Csk^j1D8, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Src42A^Scer\UAS.cPa (Pedraza et al., 2004) Csk^j1D8, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Src64B^Scer\UAS.cPa (Pedraza et al., 2004) Csk^j1D8/Csk[+] is an enhancer of eye phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Saja et al., 2010) Csk^j1D8/Csk[+] is an enhancer of eye phenotype of Ret^GMR.PR (Read et al., 2005) Csk^j1D8/Csk[+] is an enhancer of eye phenotype of Ret^MEN2B.GMR (Read et al., 2005) Csk^j1D8 is an enhancer of eye \| somatic clone phenotype of Stat92E^j6C8 (Read et al., 2004) Csk^j1D8 is an enhancer of eye phenotype of os^GMR.PB (Read et al., 2004)
NOT Enhancer of
Statement Reference Csk^j1D8/Csk[+] is a non-enhancer of eye phenotype of Egfr^E1 (Read et al., 2005) Csk^j1D8/Csk[+] is a non-enhancer of eye phenotype of Ret^MEN2A.GMR (Read et al., 2005)
Suppressor of
Statement Reference Csk^j1D8/Csk[+] is a suppressor of eye phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^{i480.Scer\UAS.cGa} (Garcia-Lopez et al., 2008) Csk^j1D8 is a suppressor of eye phenotype of wts^GMR.PS (Stewart et al., 2003) Csk^j1D8 is a suppressor of ommatidium phenotype of wts^GMR.PS (Stewart et al., 2003)
NOT Suppressor of
Statement Reference Csk^j1D8/Csk[+] is a non-suppressor of eye phenotype of Egfr^E1 (Read et al., 2005) Csk^j1D8/Csk[+] is a non-suppressor of eye phenotype of Ret^MEN2A.GMR (Read et al., 2005)
Other
Statement Reference ASPP[+]/ASPP⁸, Csk^j1D8, puc^E69/puc[+] has leg phenotype (Langton et al., 2007) ASPP⁸, Csk^j1D8/Csk[+] has wing disc phenotype (Langton et al., 2007) ASPP⁸, Csk^j1D8/Csk[+] has wing phenotype (Langton et al., 2007) ASPP^d, Csk^j1D8 has wing phenotype (Langton et al., 2009) Csk^j1D8, puc^E69/puc[+] has wing phenotype (Langton et al., 2007) Csk^j1D8, puc^E69/puc[+] has wing pouch phenotype (Langton et al., 2007) Csk^j1D8, Stat92E⁰⁶³⁴⁶ has antenna \| somatic clone phenotype (Read et al., 2004, Read et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference A Csk[j1D8]/+ background enhances the visual system degeneration seen in Scer\GAL4[GMR.PF], Ppt1[Scer\UAS.cKa] flies. (Saja et al., 2010) 68% of ASPP[d] ; Csk[j1D8]/+ flies have notched wings. (Langton et al., 2009) ASPP[8]/ASPP[8] Csk[j1D8]/+ adults show a notched wing phenotype. Wing discs of ASPP[8]/ASPP[8] Csk[j1D8]/+ third instar larvae show considerable apoptosis. Neither phenotype is seen in ASPP[8]/ASPP[8] or Csk[j1D8]/+ single mutants. Btk29A[k00206]/+ largely suppresses the ectopic apoptosis in wing discs and notched wing phenotype seen in ASPP[8]/ASPP[8] Csk[j1D8]/+ animals. puc[E69]/+ Csk[j1D8]/+ double heterozygotes have a notched wing phenotype, and third larval instar wing discs show ectopic apoptosis within the wing pouch. The ectopic apoptosis seen in the wing discs of puc[E69]/+ Csk[j1D8]/+ double heterozygotes is enhanced if they are also heterozygous for ASPP[8], resulting in adults with a severely reduced wing size. These adults also have leg deformities. (Langton et al., 2007) Csk^j1D8, Stat92E^j6C8 mutant eyes are smaller than eyes mutant for Csk^j1D8 or Stat92E^j6C8 alone. In addition, adult eyes are frequently fragmented, with scars and/or patches of eye tissue separated by patches of cuticle. Stat92E⁰⁶³⁴⁶. Csk^j1D8 mutant larval eye-antennal discs frequently show reduced sizes relative to the single mutants. Mutants also frequently show a reduction or absence of developing antennal tissues. Mutant larval eye tissue also contains many apoptotic cells. (Read et al., 2004)
Xenogenetic Interactions
Statement Reference Csk[j1D8] dominantly suppresses the rough eye phenotype caused by expression of Zzzz\CTG[i480.Scer\UAS.cGa] under the control of Scer\GAL4[sev.PU]. (Garcia-Lopez et al., 2008)
Complementation & Rescue Data
Fails to complement	Csk^S017909 (Read et al., 2004) Csk^S030003 (Read et al., 2004)
Rescued by	Csk^j1D8 is rescued by Csk^hs.PR (Read et al., 2004) Csk^j1D8 is rescued by Csk^hs.PS (Stewart et al., 2003)
Comments
Stocks ( 2 )
Bloomington	10244 y¹ w¹¹¹⁸; P{3'lacW}Csk^j1D8/TM3, Sb¹
Kyoto	111035 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}82B P{3'lacW}Csk^j1D8/TM6B, P{Car20y}TPN1, Tb¹
Notes on Origin
Discoverer

Comments
	Complements: l(3)04629⁰⁴⁶²⁹. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	Csk^j1D8 (O'Reilly et al., 2006, Christensen et al., 2008.4.15, Saja et al., 2010) dcsk1D8 (Pedraza et al., 2004) dCsk^1jd8 (Langton et al., 2007) dcsk^flik (Stewart et al., 2003) dCsk^j1D8 (Read et al., 2005, Read et al., 2004) dCsk^j1d8 (Langton et al., 2009) l(3)j1D8 (Kusano et al., 2001, Spradling et al., 1999, Garcia-Lopez et al., 2008) l(3)j1D8^j1D8 (BDGP Project Members, 1994-1999, )
Name Synonym	l(3)j1D8^j1D8 (Stewart et al., 2003)
Secondary FlyBase IDs
	FBal0086976
References ( 14 )
Research paper	Saja et al., 2010, Neurobiol. Disease 40(1): 135--145 Identifying cellular pathways modulated by Drosophila palmitoyl-protein thioesterase 1 function. [FBrf0211567] Langton et al., 2009, Curr. Biol. 19(23): 1969--1978 The dASPP-dRASSF8 complex regulates cell-cell adhesion during drosophila retinal morphogenesis. [FBrf0209661] Garcia-Lopez et al., 2008, PLoS ONE 3(2): e1595 Genetic and chemical modifiers of a CUG toxicity model in Drosophila. [FBrf0210239] Langton et al., 2007, Dev. Cell 13(6): 773--782 Drosophila ASPP regulates C-terminal Src kinase activity. [FBrf0202129] O'Reilly et al., 2006, Development 133(14): 2627--2638 Csk differentially regulates Src64 during distinct morphological events in Drosophila germ cells. [FBrf0194413] Vidal et al., 2006, Dev. Cell 10(1): 33--44 Csk-deficient boundary cells are eliminated from normal Drosophila epithelia by exclusion, migration, and apoptosis. [FBrf0190225] Read et al., 2005, Genetics 171(3): 1057--1081 A Drosophila model of multiple endocrine neoplasia type 2. [FBrf0190765] Pedraza et al., 2004, Oncogene 23(27): 4754--4762 Drosophila Src-family kinases function with Csk to regulate cell proliferation and apoptosis. [FBrf0179378] Read et al., 2004, Mol. Cell. Biol. 24(15): 6676--6689 Drosophila C-terminal Src kinase negatively regulates organ growth and cell proliferation through inhibition of the Src, Jun N-terminal kinase, and STAT pathways. [FBrf0179404] Stewart et al., 2003, Oncogene 22(41): 6436--6444 A genetic screen for modifiers of the lats tumor suppressor gene identifies C-terminal Src kinase as a regulator of cell proliferation in Drosophila. [FBrf0162272] Kusano et al., 2001, Science 291(5513): 2600--2602 Sterility of Drosophila with mutations in the Bloom syndrome gene-complementation by Ku70. [FBrf0135945] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Personal communication to FlyBase	Christensen et al., 2008.4.15, Isolation and characterization of Df(3R)BSC469. Isolation and characterization of Df(3R)BSC469. [FBrf0204547] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\Cskj1D8

Allele Dmel\Csk^j1D8