Open Close
General Information
Symbol
Dmel\Atg7dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0176389
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Atg7-RNAi, UAS-atg7RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Inverted repeat construct, with 3'-5'.5'-3' inverted repeats whose expression is driven by UASt.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adults expressing Atg7dsRNA.UAS under the control of Scer\GAL4hs.PB infected with ZIKV present an increased viral load in the head, as compared to infected controls.

Expression of Atg7dsRNA.Scer\UAS RNAi under the control of Scer\GAL4C57 does not cause any obvious defects in lysosomal tubular network in the sarcoplasm of third instar larval muscle.

Expression of Atg7dsRNA.Scer\UAS under the control of either Scer\GAL4esg-NP5130 or Scer\GAL4Myo31DF-NP0001 results in increased intestinal stem cell proliferation in response to paraquat or Ecc15.

Flies expressing Atg7dsRNA.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR in the presence of RU486 show reduced lysotracker red staining of the adult midgut compared to controls in both fed and fasting flies.

Adult expression of Atg7dsRNA.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR in the presence of RU486 has no effect on adult lifespan.

The lifespan of flies injected with E.coli is significantly reduced by expression of Atg7dsRNA.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR in the presence of RU486. These flies show a significant increase in bacterial load compared to control flies.

Flies expressing Atg7dsRNA.Scer\UAS under the control of Scer\GAL4Act are more sensitive to infection by Vesicular Stomatitis Virus than control flies, and succumb to the virus.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Atg7dsRNA.Scer\UAS is unable to suppress the rough eye phenotype and third instar larval eye disc cell death seen when Atg9Scer\UAS.T:Zzzz\FLAG is expressed under the control of Scer\GAL4GMR.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Atg7dsRNA.Scer\UAS
Atg7dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (11)