FlyBase Allele Report: Dmel\Atg5[RNAi.UAS]

General Information

Symbol

Dmel\Atg5^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0176391

Feature type

allele

Associated gene

Dmel\Atg5

Associated Insertion(s)

Carried in Construct

P{UAS-Atg5.RNAi}

Also Known As

UAS-Atg5-RNAi, UAS-atg5^RNAi, Atg5-RNAI, UAS-Atg5IR, UAS-Atg5 RNAi, Atg5 RNAi

Key Links

Transgenic product class

RNAi_reagent

(Scott et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Scott et al., 2004)

Mutagen

in vitro construct

(Scott et al., 2004, Scott et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Atg5.RNAi}

Cytology

Description

Inverted repeat construct, with 3'-5'.5'-3' inverted repeats whose expression is driven by UASt.

(Scott et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atg5

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

fat body, with Scer\GAL4^fat

(Scott et al., 2004)

Detailed Description

Statement

Reference

The expression of Atg5^dsRNA.UAS under the control of Scer\GAL4^arm.PU does not affect sensitivity to starvation.

(Bourouis et al., 2019)

Adults expressing Atg5^dsRNA.UAS under the control of either Scer\GAL4^hs.PB, Scer\GAL4^elav-C155 or Scer\GAL4^repo infected with ZIKV present an increased viral load in the head, as compared to infected controls.

(Liu et al., 2018)

Expressing Atg5^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 has no effect on the increase in sleep duration seen in wild type flies in response to rapamycin.

(Metaxakis et al., 2014)

Expression of Atg5^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ChAT.7.4 results in a significant decrease in life span as compared to control flies.

(Ling et al., 2009)

Flies expressing Atg5^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Act5C.Switch.PR} in the presence of RU486 show reduced lysotracker red staining of the adult midgut compared to controls in both fed and fasting flies.

Adult expression of Atg5^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Act5C.Switch.PR} in the presence of RU486 has no effect on adult lifespan.

The lifespan of flies injected with E.coli is significantly reduced by expression of Atg5^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Act5C.Switch.PR} in the presence of RU486. These flies show a significant increase in bacterial load compared to control flies.

(Ren et al., 2009)

Constitutive induction of Atg5 has no sigificant effect on the size or growth rate of wild type animals. Clonal expression in the fat body causes hypersensitivity to starvation. Inhibition of Atg5 causes rapamycin sensitivity.

(Scott et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Atg5^RNAi.UAS/Scer\GAL4^αTub84B.PL is an enhancer of abnormal cell size phenotype of mTor^ΔP

(Scott et al., 2004)

Atg5^RNAi.UAS/Scer\GAL4^αTub84B.PL is an enhancer of abnormal developmental rate phenotype of mTor^ΔP

(Scott et al., 2004)

NOT Enhancer of

Statement

Reference

Atg5^RNAi.UAS, Scer\GAL4^Act5C.PP is a non-enhancer of increased cell death | somatic clone phenotype of Pten¹¹⁷, Scer\GAL4^Act5C.PP, slif^UY681

(Nowak et al., 2013)

Atg5^RNAi.UAS, Scer\GAL4^Act5C.PP is a non-enhancer of increased cell growth rate | somatic clone phenotype of Pten¹¹⁷, Scer\GAL4^Act5C.PP, slif^UY681

(Nowak et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}/Atg5^RNAi.UAS is a suppressor of long lived phenotype of chico¹

(Bjedov et al., 2020)

Atg5^RNAi.UAS/Scer\GAL4^arm.PU is a suppressor of abnormal starvation stress response | adult stage phenotype of vap^KS67

(Bourouis et al., 2019)

Atg5^RNAi.UAS/Scer\GAL4^arm.PU is a suppressor of short lived | calorie restriction conditional phenotype of vap^KS67

(Bourouis et al., 2019)

Atg5^RNAi.UAS, Scer\GAL4^Tub.PU is a suppressor of lethal phenotype of Acn^UAS.Tag:MYC, Scer\GAL4^Tub.PU

(Haberman et al., 2010)

Atg5^RNAi.UAS/Scer\GAL4^ChAT.7.4 is a suppressor of short lived phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Ling et al., 2009)

Other

Statement

Reference

Atg5^RNAi.UAS, Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage phenotype

(Bilen and Bonini, 2007)

Phenotype Manifest In

Enhancer of

Statement

Reference

Atg5^RNAi.UAS, Scer\GAL4^GMR.PU is an enhancer of retina | temperature conditional phenotype of Gug^75QN.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Atg5^RNAi.UAS, Scer\GAL4^GMR.PU is an enhancer of retina | temperature conditional phenotype of Gug^66QC.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Atg5^RNAi.UAS, Scer\GAL4^GMR.PU is an enhancer of retina | temperature conditional phenotype of Gug^UAS.cCa, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Atg5^RNAi.UAS/Scer\GAL4^αTub84B.PL is an enhancer of fat body phenotype of mTor^ΔP

(Scott et al., 2004)

Suppressor of

Statement

Reference

Atg5^RNAi.UAS, Scer\GAL4^Lsp2.PH is a suppressor of embryonic/larval fat body phenotype of Acn^UAS.Tag:MYC, Scer\GAL4^Lsp2.PH

(Haberman et al., 2010)

Other

Statement

Reference

Atg5^RNAi.UAS, Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has retina phenotype

(Bilen and Bonini, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Knockdown of Atg5, through expression of Atg5^{dsRNA.Scer\UAS}, does not impact on the initial growth of Pten¹¹⁷ clones with reduced slif function (through expression of slif^UY681).

(Nowak et al., 2013)

The ability of Acn^{Scer\UAS.T:Hsap\MYC} expressed under the control of Scer\GAL4^Lsp2.PH to induce autolysosome formation in the fat body in the absence of starvation is suppressed by co-expression of Atg5^{dsRNA.Scer\UAS}.

(Haberman et al., 2010)

Expression of Gug^Scer\UAS.cCa in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5^{dsRNA.Scer\UAS}.

Expression of Gug^{66QC.Scer\UAS} in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5^{dsRNA.Scer\UAS}.

Expression of Gug^{75QN.Scer\UAS} in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5^{dsRNA.Scer\UAS}.

(Nisoli et al., 2010)

Xenogenetic Interactions

Statement

Reference

Co-expression of Atg5^{dsRNA.Scer\UAS} leads to partial suppression of the lifespan reduction seen in flies expressing Hsap\APP^{Aβ42.Scer\UAS} under the control of Scer\GAL4^ChAT.7.4.

(Ling et al., 2009)

Flies co-expressing Atg5^{dsRNA.Scer\UAS} and Hsap\ATXN3^{fl.Q84.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU show loss of retinal integrity.

(Bilen and Bonini, 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Atg5^RNAi.UAS

Atg5^{dsRNA.Scer\UAS}

Atg5^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (26)

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