Allele Dmel\Atg12^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Atg12^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0176393
Feature type	allele	Associated gene	Dmel\Atg12

Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Scott et al., 2004, Scott et al., 2004) in vitro construct - RNAi (Scott et al., 2004, Scott et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Inverted repeat construct, with 3'-5'.5'-3' inverted repeats whose expression is driven by Scer\UAS. (Scott et al., 2004)
Carried in construct	P{UAS-Atg12.IR} (Berry and Baehrecke, 2007, Pandey et al., 2007, Scott et al., 2004, Scott et al., 2004, Chen et al., 2008, Tang et al., 2011, Ren et al., 2009, Shelly et al., 2009, Batlevi et al., 2010, McPhee et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective \| RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR} (Ren et al., 2009) locomotor behavior defective, with Scer\GAL4^D42 (Batlevi et al., 2010)
Phenotype Manifest In
	autophagic vacuole \| somatic clone \| calorie restriction conditional, with Scer\GAL4^Act5C.PP (Tang et al., 2011) embryonic/larval salivary gland, with Scer\GAL4^fkh.PH (McPhee et al., 2010) fat body \| somatic clone \| calorie restriction conditional, with Scer\GAL4^Act5C.PP (Tang et al., 2011)
Detailed Description
	Statement Reference Clonal expression of Atg12[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.PP] appears to block the formation of autophagy-related vesicles in larval fat bodies under starvation conditions compared with wild-type. (Tang et al., 2011) Expression of Atg12[dsRNA.Scer\UAS] in the motor neurons of third instar larvae under the control of Scer\GAL4[D42], or in muscles under the control of Scer\GAL4[C57], results in larval motility defects. (Batlevi et al., 2010) Animals expressing Atg12[dsRNA.Scer\UAS] in the salivary glands under the control of Scer\GAL4[fkh.PH] display persistent salivary gland cell fragments in 81% of pupae. (McPhee et al., 2010) Flies expressing Atg12[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.Switch.PR] in the presence of RU486 show reduced lysotracker red staining of the adult midgut compared to controls in both fed and fasting flies. Adult expression of Atg12[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.Switch.PR] in the presence of RU486 has no effect on adult lifespan. The lifespan of flies injected with E.coli is significantly reduced by expression of Atg12[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.Switch.PR] in the presence of RU486. These flies show a significant increase in bacterial load compared to control flies. (Ren et al., 2009) When expression is driven in the fat body results in a substantial decrease in the nuimber of autolysisones. (Scott et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of visible \| drug conditional phenotype of Hsap\AR^Q52.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Pandey et al., 2007) Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of visible \| heat sensitive phenotype of Prosβ2^1.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Pandey et al., 2007)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^fkh.PH has embryonic/larval salivary gland phenotype, non-enhanceable by drpr^{dsRNA.I.Scer\UAS}, Scer\GAL4^fkh.PH (McPhee et al., 2010)
Suppressed by
Statement Reference Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PP has autophagic vacuole \| somatic clone \| calorie restriction conditional phenotype, suppressible by sqa^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PP (Tang et al., 2011) Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PP has autophagic vacuole \| somatic clone \| calorie restriction conditional phenotype, suppressible by sqa^{T279A.Scer\UAS}, Scer\GAL4^Act5C.PP (Tang et al., 2011) Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PP has autophagic vacuole \| somatic clone \| calorie restriction conditional phenotype, suppressible by sqh^{T20A.S21A.Scer\UAS}, Scer\GAL4^Act5C.PP (Tang et al., 2011) Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PP has fat body \| somatic clone \| calorie restriction conditional phenotype, suppressible by sqa^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PP (Tang et al., 2011) Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PP has fat body \| somatic clone \| calorie restriction conditional phenotype, suppressible by sqa^{T279A.Scer\UAS}, Scer\GAL4^Act5C.PP (Tang et al., 2011) Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PP has fat body \| somatic clone \| calorie restriction conditional phenotype, suppressible by sqh^{T20A.S21A.Scer\UAS}, Scer\GAL4^Act5C.PP (Tang et al., 2011)
Enhancer of
Statement Reference Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye \| drug conditional phenotype of Hsap\AR^Q52.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Pandey et al., 2007) Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye \| heat sensitive phenotype of Prosβ2^1.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Pandey et al., 2007)
Suppressor of
Statement Reference Atg12^{dsRNA.Scer\UAS}, Scer\GAL4^fkh.PH is a suppressor of embryonic/larval salivary gland phenotype of Atg1^GS10797, Scer\GAL4^fkh.PH (Berry and Baehrecke, 2007)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of sqh[T20A.S21A.Scer\UAS] with Atg12[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.PP] strongly suppresses the Atg12[dsRNA.Scer\UAS]-induced fat body phenotype. Co-expression of sqa[T279A.Scer\UAS] with Atg12[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.PP] significantly suppresses the Atg12[dsRNA.Scer\UAS]-induced fat body phenotype. Co-expression of sqa[dsRNA.Scer\UAS] with Atg12[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.PP] significantly suppresses the Atg12[dsRNA.Scer\UAS]-induced fat body phenotype. (Tang et al., 2011) Animals expressing Atg12[dsRNA.Scer\UAS] and drpr[dsRNA.I.Scer\UAS] in the salivary glands under the control of Scer\GAL4[fkh.PH] display persistence of salivary gland cell fragments in 100% of pupae, and the phenotype is very similar to expression of Atg12[dsRNA.Scer\UAS] alone. There is no increase in the amount of salivary gland material that persists, and multi-cell gland fragments are absent. (McPhee et al., 2010) Co-expression of Atg12^{dsRNA.Scer\UAS} suppresses the premature degradation of the salivary glands that is seen in animals expressing Atg1^GS10797 under the control of Scer\GAL4^fkh.PH. (Berry and Baehrecke, 2007) The rough eye phenotype seen in flies expressing Prosβ2^1.Scer\UAS under the control of Scer\GAL4^GMR.PF at 28^oC is enhanced by co-expression of Atg12^{dsRNA.Scer\UAS}. The ability of HDAC6^{Scer\UAS.T:SV5\V5} to suppress the rough eye phenotype seen in flies expressing Prosβ2^1.Scer\UAS under the control of Scer\GAL4^GMR.PF at 28^oC is suppressed by co-expression of Atg12^{dsRNA.Scer\UAS}. (Pandey et al., 2007)
Xenogenetic Interactions
Statement Reference The rough eye phenotype seen in flies expressing Hsap\AR^Q52.Scer\UAS under the control of Scer\GAL4^GMR.PF in the presence of dihydrotestosterone (DHT) is enhanced by co-expression of Atg12^{dsRNA.Scer\UAS}. The ability of HDAC6^{Scer\UAS.T:SV5\V5} to suppress the rough eye phenotype seen in flies expressing Hsap\AR^Q52.Scer\UAS under the control of Scer\GAL4^GMR.PF in the presence of dihydrotestosterone (DHT) is suppressed by co-expression of Atg12^{dsRNA.Scer\UAS}. (Pandey et al., 2007)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Atg12^{dsRNA.Scer\UAS} atg12^KD (Pandey et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Tang et al., 2011, EMBO J. 30(4): 636--651 Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy. [FBrf0213047] Batlevi et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(2): 742--747 Dynein light chain 1 is required for autophagy, protein clearance, and cell death in Drosophila. [FBrf0209730] McPhee et al., 2010, Nature 465(7301): 1093--1096 Activation of autophagy during cell death requires the engulfment receptor Draper. [FBrf0211145] Ren et al., 2009, Exp. Gerontol. 44(3): 228--235 Conditional inhibition of autophagy genes in adult Drosophila impairs immunity without compromising longevity. [FBrf0206988] Shelly et al., 2009, Immunity 30(4): 588--598 Autophagy is an essential component of Drosophila immunity against vesicular stomatitis virus. [FBrf0207677] Chen et al., 2008, Autophagy 4(1): 37--45 Genetic interactions between Drosophila melanogaster Atg1 and paxillin reveal a role for paxillin in autophagosome formation. [FBrf0203068] Berry and Baehrecke, 2007, Cell 131(6): 1137--1148 Growth arrest and autophagy are required for salivary gland cell degradation in Drosophila. [FBrf0200408] Pandey et al., 2007, Nature 447(7146): 859--863 HDAC6 rescues neurodegeneration and provides an essential link between autophagy and the UPS. [FBrf0201144] Scott et al., 2004, Dev. Cell 7(2): 167--178 Role and regulation of starvation-induced autophagy in the Drosophila fat body. [FBrf0180117]
Supplementary material	Scott et al., 2004, Dev. Cell 7(2): Supplementary material. [FBrf0183440]

Allele Dmel\Atg12dsRNA.Scer\UAS

Allele Dmel\Atg12^{dsRNA.Scer\UAS}