Allele Dmel\zwilch¹²²⁹

General Information
Symbol	Dmel\zwilch¹²²⁹	Species	D. melanogaster
Name		FlyBase ID	FBal0177077
Feature type	allele	Associated gene	Dmel\zwilch

Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Williams et al., 2003)
Mutagen	ethyl methanesulfonate (Williams et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Contains a nonsense mutation that would shorten any translated protein from 644 to 603 amino acid residues. (Williams et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile \| recessive (Williams et al., 2003) meiotic cell cycle defective (Williams et al., 2003) mitotic cell cycle defective (Williams et al., 2003)
Phenotype Manifest In
	anaphase & condensed nuclear chromosome (Williams et al., 2003)
Detailed Description
	Statement Reference The brains of homozygous zwilch¹²²⁹ larvae have many aneuploid cells. Some zwilch¹²²⁹ larval brain cells in anaphase show aberrant chromosomes that lag at the metaphase plate, while others show an unequal distribution of chromosomes between the two spindle poles. However, chromosome congression to the metaphase plate and the morphology of both the spindle and chromosomes appears normal. Unlike wild-type cells, zwilch¹²²⁹ mutant cells are not arrested in metaphase in response to treatment with colchicine, suggesting that the mutants have a failure of the spindle checkpoint. Meiosis is also affected in zwilch¹²²⁹ mutants, as zwilch¹²²⁹ testes show high levels of chromosome missegregation during both meiotic divisions. (Williams et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference zwilch¹²²⁹/zwilch¹²²⁹ is a suppressor \| partially of mitotic cell cycle defective phenotype of nudE^39A (Wainman et al., 2009, Wainman et al., 2009)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The mitotic index and the proportion of mitotic figures in anaphase relative to those in prometaphase or metaphase is nearly normal in the brains of nudE[39A] zwilch[1229] double homozygous larvae. (Wainman et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	zwilch¹²²⁹ (Wainman et al., 2009) zwilch^mit-1229 (Williams et al., 2003)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Wainman et al., 2009, J. Cell Sci. 122(11): 1747--1758 Roles of the Drosophila NudE protein in kinetochore function and centrosome migration. [FBrf0207980] Williams et al., 2003, Mol. Biol. Cell 14(4): 1379--1391 Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions. [FBrf0159187]

Allele Dmel\zwilch1229

Allele Dmel\zwilch¹²²⁹