Allele Dmel\zip^{Scer\UAS.T:Avic\GFP}

General Information
Symbol	Dmel\zip^{Scer\UAS.T:Avic\GFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0177086
Feature type	allele	Associated gene	Dmel\zip

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Barros et al., 2003) in vitro construct - regulatory fusion (Barros et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of an N-terminal fragment of zip tagged with T:Avic\GFP. (Barros et al., 2003)
Carried in construct	P{UAS-zip.GFP} (Barros et al., 2003, Okumura et al., 2010)
Tagged with	T:Avic\GFP (Barros et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^da.G32/zip^{Scer\UAS.T:Avic\GFP} rescues zip³ (Okumura et al., 2010) zip^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^wg.PM rescues zip³ (Okumura et al., 2010) zip^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^wg.PM rescues zip⁴ (Okumura et al., 2010)
Partially rescues	Scer\GAL4^how-24B, Scer\GAL4^how-24B, zip^{Scer\UAS.T:Avic\GFP} partially rescues zip³ (Okumura et al., 2010) Scer\GAL4^NetB-NP1522/zip^{Scer\UAS.T:Avic\GFP} partially rescues zip³ (Okumura et al., 2010) zip^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^48Y partially rescues zip³ (Okumura et al., 2010) zip^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^Gap1-NP3392 partially rescues zip³ (Okumura et al., 2010) zip^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} partially rescues zip¹ (Barros et al., 2003)
Fails to rescue	Scer\GAL4^byn-Gal4/zip^{Scer\UAS.T:Avic\GFP} fails to rescue zip³ (Okumura et al., 2010) Scer\GAL4^drm.7.1/zip^{Scer\UAS.T:Avic\GFP} fails to rescue zip³ (Okumura et al., 2010) Scer\GAL4^elav-C155/zip^{Scer\UAS.T:Avic\GFP} fails to rescue zip³ (Okumura et al., 2010) Scer\GAL4^NP221/zip^{Scer\UAS.T:Avic\GFP} fails to rescue zip³ (Okumura et al., 2010) Scer\GAL4^NP5021/zip^{Scer\UAS.T:Avic\GFP} fails to rescue zip³ (Okumura et al., 2010) Scer\GAL4^tey-5053A/zip^{Scer\UAS.T:Avic\GFP} fails to rescue zip³ (Okumura et al., 2010) zip^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^wg.PM fails to rescue zip³ (Okumura et al., 2010)
Comments	zip[Scer\UAS.T:Avic\GFP] driven by Scer\GAL4[da.G32] rescues the loss of rotation in the proventriculus and anterior midgut seen in zip[3] homozygotes. zip[Scer\UAS.T:Avic\GFP] driven by the circular visceral muscle drivers Scer\GAL4[how-24B], Scer\GAL4[Gap1-NP3392], Scer\GAL4[48Y] or Scer\GAL4[NetB-NP1522] partially rescues the loss of rotation in the proventriculus and anterior midgut seen in zip[3] homozygotes. zip[Scer\UAS.T:Avic\GFP] driven by the longitudinal visceral muscle drivers Scer\GAL4[NP221] or Scer\GAL4[tey-5053A] fails to rescue the loss of rotation in the proventriculus and anterior midgut seen in zip[3] homozygotes. zip[Scer\UAS.T:Avic\GFP] driven by the drivers Scer\GAL4[NP5021], Scer\GAL4[drm.7.1], Scer\GAL4[byn-Gal4], Scer\GAL4[elav-C155] or Scer\GAL4[wg.PM] fails to rescue the loss of rotation in the proventriculus and anterior midgut seen in zip[3] homozygotes. Expression of zip[Scer\UAS.T:Avic\GFP] driven by Scer\GAL4[wg.PM] suppresses the dorsal closure defects found in zip[3] homozygotes although the no-rotation phenotype is not suppressed. Expression of zip[Scer\UAS.T:Avic\GFP] driven by Scer\GAL4[wg.PM] suppresses the dorsal closure defects found in zip[4] homozygotes although the no-rotation phenotype is not suppressed. Expression of zip[Scer\UAS.T:Avic\GFP] continuously in a non-permissive temperature (32[o]C), under the control of Scer\GAL4[da.G32] and Scer\GAL80[ts.αTub84B], rescues the laterality defects found in zip[3] mutants. The zip[3] rotation defects are not rescued when the 1 hour shift to the non-permissive temperature occurs from stage 14 onwards. The temperature shift applied from 16 to 17 hours after egg laying(AEL), which corresponds to stage 12, leads to the most efficient rescue of the laterality defects associated with zip[3] mutants. However, expression of zip[Scer\UAS.T:Avic\GFP] after 22 hours AEL, corresponding to stage 14, does not rescue the laterality, 'no rotation' phenotype. zip[Scer\UAS.T:Avic\GFP] driven by Scer\GAL4[how-24B] partially rescues the loss of side specific rearrangement in circular visceral muscle cells seen in zip[3] homozygotes. The failure of the circular visceral muscle midline to rotate in relation to the midline of the embryo is also partially rescued. (Okumura et al., 2010) Expression of zip^{Scer\UAS.T:Avic\GFP}, driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, allows 34% of zip¹ mutants to hatch and undergo larval development, instead of dying as embryos. (Barros et al., 2003)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	zip^{Scer\UAS.T:Avic\GFP}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Okumura et al., 2010, Dev. Biol. 344(2): 693--706 Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila. [FBrf0211383] Barros et al., 2003, Dev. Cell 5(6): 829--840 Drosophila nonmuscle myosin II promotes the asymmetric segregation of cell fate determinants by cortical exclusion rather than active transport. [FBrf0167483]

Allele Dmel\zipScer\UAS.T:Avic\GFP

Allele Dmel\zip^{Scer\UAS.T:Avic\GFP}