Allele Dmel\tinc^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\tinc^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0177455
Feature type	allele	Associated gene	Dmel\tinc

Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Hirota et al., 2005) in vitro construct - RNAi (Hirota et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of an inverted repeat of a 500bp tinc cDNA fragment (bp 52-551 of the coding sequence). The two inverted repeats are arranged in head-to-head orientation and are separated by a 282bp genomic fragment containing introns 5 and 6 of Ret. (Hirota et al., 2005)
Carried in construct	P{UAS-tinc.IR} (Hirota et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^GMR.PY (Hirota et al., 2005)
Phenotype Manifest In
	cone cell, with Scer\GAL4^GMR.PY (Hirota et al., 2005) eye photoreceptor cell, with Scer\GAL4^GMR.PY (Hirota et al., 2005) ommatidium, with Scer\GAL4^GMR.PY (Hirota et al., 2005) pigment cell, with Scer\GAL4^GMR.PY (Hirota et al., 2005) primary pigment cell, with Scer\GAL4^GMR.PY (Hirota et al., 2005)
Detailed Description
	Statement Reference Adult flies with tinc^{dsRNA.Scer\UAS} expression driven under the control of Scer\GAL4^GMR.PY (which becomes active in all differentiating ommatidial cells posterior to the morphogenetic furrow), have abnormal eyes with disorganised and fused ommatidia. Inner tangential sections show abnormalities of the photoreceptor cells, wherein most of the cells are collapsed or split. tinc^{dsRNA.Scer\UAS} expression, under the control of Scer\GAL4^ey.PH or Scer\GAL4^GMR.PY, during the earlier stages of eye development does not affect the differentiation of the photoreceptor cells. Abnormalities associated with tinc^{dsRNA.Scer\UAS} expression occur in later developmental stages. In the wild-type eye, 40 hours after puparium formation, the eight elav-positive photoreceptor cells are arranged in a circle within an ommatidium. In contrast, in the tinc^{dsRNA.Scer\UAS} eye (driven by Scer\GAL4^GMR.PY), the patterning of the photoreceptor cells is irregular. Some ommatidia are found to be fused to adjacent ones, and the spatial patterning of the photoreceptor cells is disturbed. The pigment and cone cells of flies expressing tinc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PY are also arranged in a disorganised pattern compared to wild-type. Some cone cells and primary pigment cells show deformed shapes. These phenotypes are almost completely suppressed by the introduction of the tinc^Scer\UAS.cHa transgene. (Hirota et al., 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	tinc^{dsRNA.Scer\UAS} is rescued by tinc^Scer\UAS.cHa/Scer\GAL4^GMR.PY (Hirota et al., 2005)
Comments	tinc^Scer\UAS.cHa, under the control of Scer\GAL4^GMR.PY completely rescues the disorganisation of the ommatidial array and the morphology of the photoreceptor cells found in flies in which tinc^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PY. (Hirota et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	P{UAS-tinc.IR}, transfected into S2 cells and used to study the phenotypic consequences of dsRNA interference (RNAi) of the tinc gene. (Hirota et al., 2005)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	tinc^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Hirota et al., 2005, Dev. Genes Evol. 215(2): 90--96 The transmembrane protein, Tincar, is involved in the development of the compound eye in Drosophila melanogaster. [FBrf0183916]

Allele Dmel\tincdsRNA.Scer\UAS

Allele Dmel\tinc^{dsRNA.Scer\UAS}