|Feature type||allele||Associated gene||Dmel\tinc|
|Mutagen||in vitro construct - RNAi, in vitro construct - regulatory fusion|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Construct: Scer\UAS regulatory sequences drive expression of an inverted repeat of a 500bp tinc cDNA fragment (bp 52-551 of the coding sequence). The two inverted repeats are arranged in head-to-head orientation and are separated by a 282bp genomic fragment containing introns 5 and 6 of Ret.
|Carried in construct|
|Phenotype Manifest In|
Adult flies with tincdsRNA.Scer\UAS expression driven under the control of Scer\GAL4GMR.PY (which becomes active in all differentiating ommatidial cells posterior to the morphogenetic furrow), have abnormal eyes with disorganised and fused ommatidia. Inner tangential sections show abnormalities of the photoreceptor cells, wherein most of the cells are collapsed or split. tincdsRNA.Scer\UAS expression, under the control of Scer\GAL4ey.PH or Scer\GAL4GMR.PY, during the earlier stages of eye development does not affect the differentiation of the photoreceptor cells. Abnormalities associated with tincdsRNA.Scer\UAS expression occur in later developmental stages. In the wild-type eye, 40 hours after puparium formation, the eight elav-positive photoreceptor cells are arranged in a circle within an ommatidium. In contrast, in the tincdsRNA.Scer\UAS eye (driven by Scer\GAL4GMR.PY), the patterning of the photoreceptor cells is irregular. Some ommatidia are found to be fused to adjacent ones, and the spatial patterning of the photoreceptor cells is disturbed. The pigment and cone cells of flies expressing tincdsRNA.Scer\UAS under the control of Scer\GAL4GMR.PY are also arranged in a disorganised pattern compared to wild-type. Some cone cells and primary pigment cells show deformed shapes. These phenotypes are almost completely suppressed by the introduction of the tincScer\UAS.cHa transgene.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 1 )|