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General Information
Symbol
Dmel\stiGS9053
Species
D. melanogaster
Name
FlyBase ID
FBal0177615
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Insertion oriented to drive expression in the sense direction starting upstream of the coding region.

    Insertion components
    P{GSV6}stiGS9053
    Product class / Tool use(s)
    Encoded product / tool
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Contractile ring dynamics in stiGS9053/Df(3L)iro-2 mutant neuroblasts are similar to controls, albeit less uniform, indicating that the defects in contractile ring organization observed in the absence of sti occur after and not during cleavage furrow ingression.

    Expression of stiGS9053 under the control of Scer\GAL4GMR.PF results in a wild type eye.

    Stage 16 stiGS9053/Df(3L)iro-2 embryos have a modestly disrupted peripheral nervous system (PNS) with some disorganisation of clusters and defects in axonal projections. There is also a reduction in the numbers of PNS neurons in these embryos, and concomitant appearance of binucleate neurons (about 15% of total neurons are binucleate). About 1/4 of cells in the brains of third instar stiGS9053/Df(3L)iro-2 larvae have massively enlarged nuclei. Chromosome preparations from these brains show tetraploid metaphase figures and some massively hyperploid cells. As chromosomes seem to separate normally in all except the most polyploid of cells, the defect appears to be in cytokinesis, rather than some other aspect of mitosis. These larvae also exhibit a variable reduction in imaginal disc size. This is associated with an increase in apoptosis in the imaginal discs and a decrease in numbers of cells expressing mitotic markers.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    stiGS9053/Df(3L)iro-2 has embryonic peripheral nervous system & neuron phenotype, enhanceable by pbl[+]/pbl3

    Enhancer of
    NOT Enhancer of
    Statement
    Reference

    stiGS9053/Scer\GAL4en-e16E is a non-enhancer of wing | posterior & wing hair phenotype of Scer\GAL4en-e16E, pbldsRNA.UAS

    Suppressor of
    NOT Suppressor of
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The The reduction in PNS neuron numbers and the percentage of binucleate PNS neurons in stiGS9053/Df(3L)iro-2 embryos are both enhanced by pbl3/+. When cell death is inhibited in stiGS9053/Df(3L)iro-2 third instar discs by the presence of BacA\p35Scer\UAS.cHa, multinucleate cells accumulate.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer

    Toshiro Aigaki

    Comments
    Comments

    Lethality over Df(3L)iro-2 is revertable with Δ2-3. However, original stock carries 2nd site lethal on 3R.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    Name Synonyms
    Secondary FlyBase IDs
      References (4)