FlyBase Allele Report: Dmel\spas[5.75]

General Information

Symbol

Dmel\spas^5.75

Species

D. melanogaster

Name

FlyBase ID

FBal0177736

Feature type

allele

Associated gene

Dmel\spas

Associated Insertion(s)

Carried in Construct

Also Known As

spastin^5.75

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Sherwood et al., 2004)

Mutagen

Delta2-3 transposase

(Sherwood et al., 2004)

Progenitor genotype

P{EP}spas^T32

(Sherwood et al., 2004)

Caused by aberration

Df(3R)spas^5.75

Cytology

Description

Imprecise excision of the P{EP}spas^T32 insertion has created a 5.82kb deletion that spans from the insertion site to remove the whole of the spas gene.

(Sherwood et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of hereditary spastic paraplegia

CEA

(Baxter et al., 2014, Du et al., 2010, Sherwood et al., 2004)

CEA with Hsap\SPAST^{R388.UAS.Venus}

(Du et al., 2010)

model of hereditary spastic paraplegia 4

CEA

(Ozdowski et al., 2020, Lee et al., 2009)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of hereditary spastic paraplegia

is ameliorated by Hsap\SPAST^UAS.Venus

(Du et al., 2010)

is ameliorated by Pak3^d02472

(Ozdowski et al., 2011)

is ameliorated by Pak3^Df

(Ozdowski et al., 2011)

ameliorates fragile X syndrome

modeled by Fmr1^EP3517

(Yao et al., 2011)

modeled by Fmr1^sev.PW

(Yao et al., 2011)

model of hereditary spastic paraplegia 4

is ameliorated by Pak3^Df

(Ozdowski et al., 2020)

is ameliorated by Pak3^GD8481

(Ozdowski et al., 2020)

is ameliorated by Pak3^GL00287

(Ozdowski et al., 2020)

is ameliorated by Pak3^NIG.14895R

(Ozdowski et al., 2020)

is ameliorated by Pak3^d02472

(Ozdowski et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Sherwood et al., 2004)

abnormal eclosion

(Du et al., 2010)

abnormal heat stress response

(Stewart et al., 2012)

abnormal locomotor behavior

(Sherwood et al., 2004)

abnormal locomotor behavior | adult stage

(Du et al., 2010)

abnormal locomotor behavior | progressive (with spas^17-7)

(Madabattula et al., 2015)

abnormal neuroanatomy

(Ozdowski et al., 2011, Jinushi-Nakao et al., 2007)

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav.PU, spas^K467R.UAS

(Orso et al., 2005)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PU

(Orso et al., 2005)

abnormal neurophysiology

(Ozdowski et al., 2011, Sherwood et al., 2004)

abnormal neurophysiology (with spas^17-7)

(Sherwood et al., 2004)

abnormal size | dominant | larval stage

(Rao et al., 2016)

abnormal size | larval stage (with spas^10-12)

(Rao et al., 2016)

abnormal stress response

(Stewart et al., 2012)

abnormal wound healing | dominant | larval stage

(Rao et al., 2016)

abnormal wound healing | larval stage (with spas^10-12)

(Rao et al., 2016)

flightless

(Sherwood et al., 2004)

lethal - all die before end of pupal stage

(Sherwood et al., 2004)

partially lethal - majority die

(Du et al., 2010)

partially lethal - majority die, with Scer\GAL4^Tub.PU

(Orso et al., 2005)

partially lethal - majority die | heat sensitive

(Baxter et al., 2014)

short lived

(Du et al., 2010, Sherwood et al., 2004)

some die during pupal stage

(Du et al., 2010, Sherwood et al., 2004)

sterile

(Sherwood et al., 2004)

Phenotype Manifest In

adult brain, with Scer\GAL4^elav.PU, spas^K467R.UAS

(Orso et al., 2005)

axon | larval stage

(Rao et al., 2016)

axon | larval stage (with spas^10-12)

(Rao et al., 2016)

bouton

(Sherwood et al., 2004)

bouton | third instar larval stage, with Scer\GAL4^elav.PU

(Orso et al., 2005)

dendrite

(Jinushi-Nakao et al., 2007)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PU

(Orso et al., 2005)

endoplasmic reticulum | larval stage (with spas^10-12)

(Rao et al., 2016)

eye photoreceptor cell | somatic clone

(Chen et al., 2010)

larval dorsal multidendritic neuron ddaC

(Jinushi-Nakao et al., 2007)

larval dorsal multidendritic neuron ddaE | larval stage

(Rao et al., 2016)

larval dorsal multidendritic neuron ddaE | larval stage (with spas^10-12)

(Rao et al., 2016)

larval multidendritic class IV neuron

(Jinushi-Nakao et al., 2007)

leg

(Sherwood et al., 2004)

microtubule

(Ozdowski et al., 2011)

microtubule, with Scer\GAL4^elav.PU

(Orso et al., 2005)

microtubule | larval stage

(Du et al., 2010)

NMJ bouton

(Ozdowski et al., 2011)

NMJ bouton | larval stage | increased number

(Du et al., 2010)

synapse | third instar larval stage, with Scer\GAL4^elav.PU

(Orso et al., 2005)

Detailed Description

Statement

Reference

Depending on the genetic background, spas^5.75/spas^10-12 transheterozygous and spas^5.75 heterozygous larval ddaE neurons exhibit defects in axon regeneration, shown by a significant decrease in axon length, but not in dendrite regeneration, shown by a similar dendrite number, at 96h after laser-induced severance, as compared to controls. Upon axon severance in spas^5.75/spas^10-12 transheterozygous larval ddaE neurons, the endoplasmic reticulum is recruited to additional neurites and less strongly to the regenerating axon, as compared to the strong accumulation only at the regenerating axon in controls. Uninjured spas^5.75/spas^10-12 transheterozygous neurons do not show endoplasmic reticulum defects, as compared to controls.

spas^5.75 heterozygous larval ddaE neurons present an apparently normal early response to injury, as the pool of growing microtubules (identified as fluorescent EB1 comets) shown by the expected reversal of polarity at 24h after laser-induced severance, as compared to controls.

(Rao et al., 2016)

spas^5.75/spas^17-7 mutants exhibit significantly decreased climbing performance at 2 days old, and climbing performance worsens by 8 days old.

(Madabattula et al., 2015)

Compared with the 86% eclosion rate of wild type flies, only 18% of homozygous spas^5.75 flies maintained at 24[o]C eclose. Exposing mutants to cold treatment (18[o]C) specifically during the larval stage results in slightly, but not significantly, higher mutant eclosion. Pupal stage cold treatment produces a significant increase in eclosion to levels that, although still well below wild type, are 70% greater than those for untreated mutants. This effect is specific to the spas^5.75 mutation, as cold does not affect wild type eclosion.

Whereas wild type flies at 24[o]C lived 3-4 weeks on average, spas^5.75 mutants survive only 1 week. Exposure to cold treatment (18[o]C) during the larval stages is deleterious to mutant fly survival. In contrast, adult-stage cold treatment extends the average lifespan 2.5-fold, but this is not different from wild type flies, which live 2.3-fold longer when cold-treated as adults. However, whereas pupal cold treatment leaves wild type flies unaffected, spas^5.75 flies live twice as long as untreated mutants.

All spas^5.75 mutants that eclose following larval stage cold treatment do not climb, dying shortly after eclosion. However, spas^5.75 flies cold-treated as pupae climb 46% faster than mutants maintained at 24[o]C, and when cold-treated as adults, climb 85% faster. By contrast, wild type climbing is unaffected by pupal and adult cold treatment, but is impaired by larval-stage treatment, although to a lesser extent than spas^5.75.

Comparison of synaptic terminals in 18[o]C- versus 24[o]C-reared larvae shows that cooling mitigates the spas^5.75 morphology, both with respect to the total number of boutons per muscle and the number of terminal boutons (a measure of synaptic arbor branching). Wild type synapse morphology is unaffected. The defects in stable microtubule distribution seen in spas^5.75 are not suppressed by cooling.

(Baxter et al., 2014)

spas^5.75 heterozygous larvae display a delayed responsiveness to a 46[o]C thermal probe. These larvae also display comparative insensitivity to a noxious mechanical stimuli, compared to controls.

Dendrites in spas^5.75 heterozygotes show no change in the number of microtubule comets compared to controls.

(Stewart et al., 2012)

The NMJs of homozygous spas^5.75 mutant larvae have a severely increased numbers of boutons, mainly at the terminals of each branch, where 'bunches' (clusters of similarly sized boutons that appear like a bunch of grapes and are found only at the distal tips of axonal branches) are prevalent. Microtubules in these boutons are diffuse at the distal tips and do not form the loops that are found in wild type boutons. The mean amplitude of evoked Excitatory Junction Potentials (EJPs) in third instar larvae is reduced compared to controls. The quantal content is also reduced, but miniature EJP (mEJP) is unaffected. mEJP frequency is increased in spas^5.75 mutants.

(Ozdowski et al., 2011)

The localisation of stable microtubules is relatively normal at the distal section of the photoreceptor in homozygous clones in the eye at 45% pupal stage, but stable microtubules are reduced at the proximal section of the photoreceptor in these clones.

(Chen et al., 2010)

Very few homozygous spas^5.75 flies eclose and the few escapers that are seen have difficulty walking and jumping and cannot fly. They are short lived compared to wild type flies. spas^5.75 flies exhibit almost double the number of neuromuscular junction boutons seen in wild type, and these boutons are small and clustered rather than linearly arranged. Mutants also display defects in terminal bouton microtubule distribution.

(Du et al., 2010)

Reduction of spas levels in a spas^5.75 heterozygous background leads to large gaps both between neighboring class IV dendritic arbors and within the arbor of an individual neuron. spas^5.75 heterozygous mutants exhibit 43% more uncovered areas of dendritic outgrowth compared to wild-type ddaC neurons.

(Jinushi-Nakao et al., 2007)

spas^5.75 mutant flies exhibit reduced eclosion rates, and this is partially rescued upon administration of the microtubule destabilizing drug vinblastine. Synaptic area is decreased, synaptic boutons are more numerous, and stable microtubules accumulate at the NMJ synapse. The administration of the microtubule destabilizing drug vinblastine partially suppresses there phenotypes.

One copy of spas^5.75 enhances the neurodegeneration seen in the adult brain when spas^{K467R.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU. Significant degeneration is seen, particularly in the medulla.

(Orso et al., 2005)

The neuromuscular junctions (NMJs) in mutant larvae are abnormal. The number of the larger Ib boutons per muscle 4 NMJ is increased 1.6 fold relative to wild-type (at about 23[o]C) and the boutons often form dense clusters, particularly at the end of NMJ branches. Homozygous mutant larvae, there is a reduction seen in the amplitudes of evoked responses (excitatory junction potentials - EJPs) to depolarisation of the innervating nerves. Average EJP amplitudes are reduced to 78% of the levels seen in controls. mini EJP (mEJP) amplitude is increased slightly to 117% of control levels. Quantal content is reduced to 67% of control levels. spas^5.75/spas^17-7 larvae, there is a reduction seen in the amplitudes of evoked responses (excitatory junction potentials - EJPs) to depolarisation of the innervating nerves. Average EJP amplitudes are reduced. Quantal content is reduced to 78% of control levels at 18[o]C, at 29[o]C the QC is 54% of wild-type. Approximately 20% of homozygous mutant pupae are able to eclose at room temperature, and those that do have severe movement defects. Homozygous adults are short lived and cannot fly at all, and do not appear to move their wings, although the wings inflate and straighten in a normal manner immediately after eclosion. Animals also do not jump spontaneously, but do jump if persistently prodded in the abdomen. Their legs are weak: when standing the metathoracic legs often slip out from underneath them and during walking they often drag these legs. They also have difficulty holding on to surfaces when they are upside down. These phenotypes are temperature dependent. Mutant animals exhibit a reduced climbing ability. Only 10% of eclosed adults are males.

(Sherwood et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

spas^5.75 has abnormal neuroanatomy phenotype, suppressible by Pak3^d02472/Pak3^Df

(Ozdowski et al., 2011)

spas^5.75 has abnormal neurophysiology phenotype, suppressible by Pak3^d02472/Pak3^Df

(Ozdowski et al., 2011)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{R388.UAS.Venus}/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{R388.UAS.Venus}/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{R388.UAS.Venus}

(Du et al., 2010)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^R431STOP.UAS

(Du et al., 2010)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP

(Du et al., 2010)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP/Hsap\SPAST^{R388.UAS.Venus}

(Du et al., 2010)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^Q45.UAS

(Du et al., 2010)

spas^5.75 has abnormal eclosion phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{R388.UAS.Venus}/Hsap\SPAST^Q45.UAS

(Du et al., 2010)

NOT suppressed by

Statement

Reference

spas^5.75 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP/Hsap\SPAST^{R388.UAS.Venus}

(Du et al., 2010)

Suppressor of

Statement

Reference

spas[+]/spas^5.75 is a suppressor of abnormal stress response phenotype of Crei\ChR2^UAS.cHa.EYFP, Scer\GAL4^ppk.1.9

(Stewart et al., 2012)

Other

Statement

Reference

Hsap\SPAST^UAS.Venus, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype

(Du et al., 2010)

Hsap\SPAST^{R388.UAS.Venus}, Hsap\SPAST^UAS.Venus, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype

(Du et al., 2010)

Hsap\SPAST^L44.UAS.CFP, Hsap\SPAST^UAS.Venus, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype

(Du et al., 2010)

Hsap\SPAST^L44.UAS.CFP, Hsap\SPAST^{R388.UAS.Venus}, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype

(Du et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

spas^5.75 has NMJ bouton phenotype, suppressible by Pak3^d02472/Pak3^Df

(Ozdowski et al., 2011)

spas^5.75 has microtubule phenotype, suppressible by Pak3^d02472/Pak3^Df

(Ozdowski et al., 2011)

spas^5.75 has NMJ bouton | increased number | larval stage phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^Q45.UAS

(Du et al., 2010)

spas^5.75 has microtubule | larval stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^Q45.UAS

(Du et al., 2010)

spas^5.75 has NMJ bouton | increased number | larval stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{R388.UAS.Venus}/Hsap\SPAST^Q45.UAS

(Du et al., 2010)

spas^5.75 has microtubule | larval stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{R388.UAS.Venus}/Hsap\SPAST^Q45.UAS

(Du et al., 2010)

spas^5.75 has NMJ bouton | increased number | larval stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has microtubule | larval stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has NMJ bouton | increased number | larval stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{R388.UAS.Venus}/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has NMJ bouton | increased number | larval stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has microtubule | larval stage phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

NOT suppressed by

Statement

Reference

spas^5.75 has microtubule | larval stage phenotype, non-suppressible by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{R388.UAS.Venus}/Hsap\SPAST^UAS.Venus

(Du et al., 2010)

spas^5.75 has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP/Hsap\SPAST^{R388.UAS.Venus}

(Du et al., 2010)

spas^5.75 has microtubule | larval stage phenotype, non-suppressible by Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^L44.UAS.CFP/Hsap\SPAST^{R388.UAS.Venus}

(Du et al., 2010)

Suppressor of

Statement

Reference

spas^5.75/spas^5.75 is a suppressor of larval somatic muscle cell phenotype of Scer\GAL4^C57, atl^UAS.cLa

(Lee et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Pak3^Df/Pak3^d02472 completely suppresses the defects in axonal branching and microtubule organisation seen in homozygous spas^5.75 larval neuromuscular junctions. Bunched synaptic boutons are also no longer observed and the reductions in Excitatory Junction Potential (EJP) amplitude and quantal content are fully rescued. In contrast to either mutant alone, the miniature EJP frequency in Pak3^Df/Pak3^d02472 spas^5.75 double mutants is similar to controls.

(Ozdowski et al., 2011)

spas^5.75 partly suppresses the microtubule phenotype seen in the muscles of animals expressing tbce^Scer\UAS.cJa under the control of Scer\GAL4^C57.

(Jin et al., 2009)

The muscle defects seen in larvae expressing atl^Scer\UAS.cLa under the control of Scer\GAL4^C57 are suppressed by spas^5.75/spas^5.75.

(Lee et al., 2009)

Xenogenetic Interactions

Statement

Reference

A spas^5.75 heterozygous background results in a reduction of the nocifensive escape locomotion response to blue-light stimulation in flies expressing Crei\ChR2^{Scer\UAS.cHa.T:Avic\GFP-EYFP} under the control of Scer\GAL4^ppk.1.9.

(Stewart et al., 2012)

Expression of Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75 homozygotes. A similar level of rescue is seen when two copies of the transgene are expressed, however increasing the concentration of RU486 leads to increased lethality. The increased bouton number phenotype is partially rescued, with the number of boutons restored to close to wild type levels. The boutons seen are arranged linearly rather than being small and clustered as in spas^5.75. Terminal bouton microtubule distribution is also rescued to close to wild type levels. Flies carrying either one or two copies of the transgene have lifespans of approximately 15 days.

Co-expression of Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} and Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75 homozygotes, although only 75% of flies eclose compared to when two copies of Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} are expressed. The movement defects seen in spas^5.75 homozygotes are also partially rescued, with rescue flies still exhibiting mild walking rate, jumping and flying defects. The bouton number phenotype is partially rescued although less efficiently than when two copies of Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} are expressed. Bouton number is reduced significantly compared to spas^5.75, but boutons seen are still small and clustered rather than linear. Terminal bouton microtubule distribution is not rescued. Flies of this genotype have a severely reduced lifespan of approximately 10 days.

Expression of Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75 homozygotes. Four times as many flies survive to adulthood as in spas^5.75.

Expression of Hsap\SPAST^{R431STOP.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75 homozygotes. Twice as many flies eclose as in spas^5.75.

Co-expression of Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} and Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^{elav.Switch.PO} restores the eclosion and movement defects seen in spas^5.75 homozygotes to a similar level as when two copies of Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} are expressed. The spas^5.75 increased bouton number phenotype is partially rescued, although bouton number is slightly higher than when Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is expressed alone. Terminal bouton microtubule distribution is also partially rescued. Flies of this genotype have an average lifespan of approximately 16 days.

Expression of Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75 homozygotes.

Co-expression of Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus} and Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75, but fails to rescue the movement defects. Neither the increase in bouton number nor the terminal bouton microtubule distribution phenotype seen in spas^5.75 is rescued. Flies of this genotype have a severely reduced lifespan of approximately 4 days.

Expression of Hsap\SPAST^Q45.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75 homozygotes. The bouton number and terminal bouton microtubule distribution defects seen in spas^5.75 are also partially rescued.

Co-expression of Hsap\SPAST^Q45.Scer\UAS and Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75 homozygotes, although the rescue is less efficient than when Hsap\SPAST^Q45.Scer\UAS is expressed alone. The bouton number and terminal bouton microtubule distribution phenotypes are also partially rescued. The number of boutons is slightly less than in spas^5.75 and those that are seen are small and clustered.

(Du et al., 2010)

Complementation and Rescue Data

Rescued by

spas^5.75 is rescued by Scer\GAL4^{elav.Switch.PO}/spas^UAS.cSa

(Sherwood et al., 2004)

spas^5.75 is rescued by spas^UAS.cSa/Scer\GAL4^spin.PN

(Sherwood et al., 2004)

Partially rescued by

spas^5.75 is partially rescued by Scer\GAL4^{elav.Switch.PO}/spas^UAS.CFP

(Du et al., 2010)

Comments

Expression of spas^{Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^{elav.Switch.PO} partially rescues the eclosion defects seen in spas^5.75 homozygotes. Increasing spas expression levels through increasing the number of transgenes or the concentration of RU486 does not further improve eclosion rates.

Expression of spas^{Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^{elav.Switch.PO} fully rescues the behavioural defects seen in spas^5.75 homozygotes. Rescued flies are able to fly, jump and crawl comparably to wild type flies.

(Du et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Dspastin^5.75-null

(Orso et al., 2005)

Spas^5.75

(Rao et al., 2016)

spas^5-75

(Julien et al., 2016)

spas^5.75

(Bu et al., 2021, Madabattula et al., 2015, Stewart et al., 2012, Lee et al., 2009, Jinushi-Nakao et al., 2007)

spastin^5.75

(Ozdowski et al., 2020, Mao et al., 2014, Yao et al., 2011, Chen et al., 2010, Du et al., 2010, Jin et al., 2009)

Name Synonyms

spastin5.75

(Baxter et al., 2014, Ozdowski et al., 2011, Jinushi-Nakao et al., 2007)

Secondary FlyBase IDs

References (17)

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