Allele Dmel\robo^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\robo^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0178178
Feature type	allele	Associated gene	Dmel\robo

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Tayler et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a robo RNAi construct. (Tayler et al., 2004)
Carried in construct	P{UAS-robo.RNAi} (Fan et al., 2005, Tayler et al., 2004, Mellert et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| adult stage \| female, with Scer\GAL4^Poxn.14 (Mellert et al., 2010) neuroanatomy defective \| adult stage \| male, with Scer\GAL4^Poxn.14 (Mellert et al., 2010)
Phenotype Manifest In
	prothoracic leg taste bristle chemosensory neuron \| female, with Scer\GAL4^Poxn.14 (Mellert et al., 2010) prothoracic leg taste bristle chemosensory neuron \| male, with Scer\GAL4^Poxn.14 (Mellert et al., 2010)
Detailed Description
	Statement Reference Males expressing robo[dsRNA.Scer\UAS] under the control of Scer\GAL4[Poxn.14] show an increase in midline crossing by foreleg gustatory receptor neuron (GRN) axons, with an increase in GRN axonal projections in the commissural region. Females expressing robo[dsRNA.Scer\UAS] under the control of Scer\GAL4[Poxn.14] often have visible crossing of the midline by foreleg GRN axons (this midline crossing is normally only seen in wild-type males, and not in wild-type females). (Mellert et al., 2010) Expression of a single copy of robo^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-375.1 has no effect on visual system development. (Tayler et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement Reference Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-enhancer of neuroanatomy defective phenotype of egh⁷ (Fan et al., 2005)
NOT Suppressor of
Statement Reference Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-suppressor of neuroanatomy defective phenotype of egh⁷ (Fan et al., 2005)
Other
Statement Reference Scer\GAL4^elav-C155, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has neuroanatomy defective phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^sca-375.1, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has neuroanatomy defective phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^sca-375.1, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has neuroanatomy defective phenotype (Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^αTub84B.PL, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has neuroanatomy defective phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004)
Phenotype Manifest In
NOT Enhancer of
Statement Reference Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-enhancer of photoreceptor cell R1 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-enhancer of photoreceptor cell R2 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-enhancer of photoreceptor cell R3 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-enhancer of photoreceptor cell R4 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-enhancer of photoreceptor cell R5 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-enhancer of photoreceptor cell R6 & axon phenotype of egh⁷ (Fan et al., 2005)
NOT Suppressor of
Statement Reference Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-suppressor of photoreceptor cell R1 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-suppressor of photoreceptor cell R2 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-suppressor of photoreceptor cell R3 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-suppressor of photoreceptor cell R4 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-suppressor of photoreceptor cell R5 & axon phenotype of egh⁷ (Fan et al., 2005) Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} is a non-suppressor of photoreceptor cell R6 & axon phenotype of egh⁷ (Fan et al., 2005)
Other
Statement Reference Scer\GAL4^elav-C155, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has lamina anlage phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^Poxn.14, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has prothoracic leg taste bristle chemosensory neuron \| male phenotype (Mellert et al., 2010) Scer\GAL4^sca-375.1, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has eye photoreceptor cell & axon phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^sca-375.1, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has lamina anlage phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^αTub84B.PL, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has eye photoreceptor cell & axon phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Males and females simultaneously co-expressing robo[dsRNA.Scer\UAS], lea[dsRNA.Scer\UAS] and robo3[dsRNA.Scer\UAS] under the control of Scer\GAL4[Poxn.14] show severe disruption of foreleg gustatory receptor neuron axon morphology, with axons collapsing onto the midline. (Mellert et al., 2010) Expression of robo^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^elav-C155, does not alter the R-cell phenotype of egh⁷ mutants. (Fan et al., 2005) Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in defects in visual system development; distal cell neurons invade the developing lamina. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-375.1 results in distal cell neurons invading the developing lamina. The distal cell neurons and lamina glia are intermingled in these animals. In addition defects in photoreceptor axon targeting are seen, with the regions of mistargeting correlating with the regions where the distal cell neurons enter the lamina. Co-expression of two copies of robo^{dsRNA.Scer\UAS} and two copies of robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-375.1 results in modest defects in the visual system. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL results in many photoreceptor axons extending through the lamina and too many photoreceptor axons entering the medulla. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF does not result in any defects in photoreceptor axon targeting. (Tayler et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	9285 w^*; P{UAS-robo.RNAi}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	robo^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Mellert et al., 2010, Development 137(2): 323--332 Midline crossing by gustatory receptor neuron axons is regulated by fruitless, doublesex and the Roundabout receptors. [FBrf0209580] Fan et al., 2005, Dev. Biol. 287(1): 61--73 The egghead gene is required for compartmentalization in Drosophila optic lobe development. [FBrf0190118] Tayler et al., 2004, Development 131(23): 5935--5945 Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins. [FBrf0180181]

Allele Dmel\robodsRNA.Scer\UAS

Allele Dmel\robo^{dsRNA.Scer\UAS}