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General Information
Symbol
Dmel\pbldsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0178473
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of a stem-loop dsRNA consisting of nucleotides 1184-2916 of the pbl EST SD01796, separated by a 298 bp spacer sequence from nucleotides 2182-2916 of the EST.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of pbldsRNA.Scer\UAS under the control of Scer\GAL4en.PU results in a disruption in development of the posterior compartment of the wing.

Expression of pbldsRNA.Scer\UAS under the control of Scer\GAL4en-e16E leads to a reduced wing size and the appearance of cells with multiple wing hairs within the posterior of the wing.

Expression of pbldsRNA.Scer\UAS under the control of Scer\GAL4en-e16E results in a reduction of the fraction of cells in G2 phase and an increase in hyperploid cells in 3rd instar wing discs.

The posterior compartment of the wings of pbldsRNA.Scer\UAS; Scer\GAL4en-e16E flies is reduced in size and more than 67% of its cells have multiple wing hairs. During pupal stages the posterior compartment of the developing wing in these animals contains abnormally large and multinucleate cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, enhanceable by Scer\GAL4en-e16E/cdc14GS9041

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, enhanceable by MbsGS13173, Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, enhanceable | heat sensitive by Rac2GS10622, Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, enhanceable | heat sensitive by CHES-1-likeGS1023, Scer\GAL4en-e16E

NOT Enhanced by
Statement
Reference

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, non-enhanceable by stiGS9053/Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, non-enhanceable by Cdk4GS2301, Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, non-enhanceable by LstGS2301, Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, non-enhanceable by Scer\GAL4en-e16E/tefuGS13617

Scer\GAL4en-e16E, pbldsRNA.UAS has wing hair | supernumerary phenotype, non-enhanceable by rok[+]/Rok2

Suppressed by
Statement
Reference

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, suppressible | partially by stgGS3120/Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, suppressible | partially by Scer\GAL4en-e16E/wunGS10009

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, suppressible | partially by Scer\GAL4en-e16E/aurAGS7173

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, suppressible | partially by PitslreGS10080, Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, suppressible | partially by Scer\GAL4en-e16E/Diap1GS9038

NOT suppressed by
Statement
Reference

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, non-suppressible by Cdk4GS2301, Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, non-suppressible by LstGS2301, Scer\GAL4en-e16E

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, non-suppressible by Scer\GAL4en-e16E/tefuGS13617

Scer\GAL4en-e16E, pbldsRNA.UAS has wing | posterior & wing hair phenotype, non-suppressible by stiGS9053/Scer\GAL4en-e16E

Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Rac2GS10622 or CHES-1-likeGS1023 in a Scer\GAL4en-e16E, pbldsRNA.Scer\UAS background results in lethality at 25oC and enhancement of the wing phenotype at 18oC.

Co-expression of Pk61CGS10133 suppresses both the G2 and hyperploid cell effects seen in Scer\GAL4en-e16E, pbldsRNA.Scer\UAS wing discs.

Co-expression of cdc14GS9041 strongly enhances the hyperploid cell fraction seen in Scer\GAL4en-e16E, pbldsRNA.Scer\UAS wing discs and reduces the fraction of cells in G1 phase.

Co-expression of thGS9038 reduces the proportion of hyperploid cells seen in Scer\GAL4en-e16E, pbldsRNA.Scer\UAS wing discs.

Co-expression of stgGS3120 does not significantly affect the hyperploid cell effects seen in Scer\GAL4en-e16E, pbldsRNA.Scer\UAS wing discs, though it does strongly push cells from the G2 to the G1 fraction.

The reduction in compartment size and the percentage of cells with multiple wing hairs in pbldsRNA.Scer\UAS; Scer\GAL4en-e16E flies are both enhanced by Rho172R/+, Df(3L)iro-2/+ or stiKG01697/+, but not by rok2/+. These phenotypes are not suppressed by stiGS9053 (note that while this is this a loss of function allele, in the presence of Scer\GAL4 it drives ectopic expression of sti.)

Xenogenetic Interactions
Statement
Reference

Co-expression of either Cele\rrf-1Scer\UAS.cDa or Cele\ego-1Scer\UAS.P\T has no effect on the defect seen in the posterior compartment of the wing in animals expressing pbldsRNA.Scer\UAS under the control of Scer\GAL4en.PU.

Co-expression of Cele\ego-1Scer\UAS.cDa suppresses the defect seen in the posterior compartment of the wing in animals expressing pbldsRNA.Scer\UAS under the control of Scer\GAL4en.PU.

Co-expression of BacA\p35Scer\UAS.cHa enhances the proportion of hyperploid cells seen in Scer\GAL4en-e16E, pbldsRNA.Scer\UAS wing discs.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
pbldsRNA.Scer\UAS
pbldsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (3)