FlyBase Allele Report: Dmel\pbl[RNAi.UAS]

General Information

Symbol

Dmel\pbl^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0178473

Feature type

allele

Associated gene

Dmel\pbl

Associated Insertion(s)

Carried in Construct

P{UAS-pbl.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Shandala et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Shandala et al., 2004)

Mutagen

in vitro construct

(Shandala et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-pbl.RNAi}

Cytology

Description

UASt sequences drive expression of a stem-loop dsRNA consisting of nucleotides 1184-2916 of the pbl EST SD01796, separated by a 298 bp spacer sequence from nucleotides 2182-2916 of the EST.

(Shandala et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

pbl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^en-e16E

(Gregory et al., 2007)

visible, with Scer\GAL4^en.PU

(Duan et al., 2013)

Phenotype Manifest In

wing | posterior, with Scer\GAL4^en-e16E

(Gregory et al., 2007)

wing | posterior & wing hair, with Scer\GAL4^en-e16E

(Gregory et al., 2007)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Shandala et al., 2004)

wing disc | third instar larval stage, with Scer\GAL4^en-e16E

(Gregory et al., 2007)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Duan et al., 2013)

wing hair | increased number, with Scer\GAL4^en-e16E

(Shandala et al., 2004)

Detailed Description

Statement

Reference

Expression of pbl^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en.PU results in a disruption in development of the posterior compartment of the wing.

(Duan et al., 2013)

Expression of pbl^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E leads to a reduced wing size and the appearance of cells with multiple wing hairs within the posterior of the wing.

Expression of pbl^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E results in a reduction of the fraction of cells in G2 phase and an increase in hyperploid cells in 3rd instar wing discs.

(Gregory et al., 2007)

The posterior compartment of the wings of pbl^{dsRNA.Scer\UAS}; Scer\GAL4^en-e16E flies is reduced in size and more than 67% of its cells have multiple wing hairs. During pupal stages the posterior compartment of the developing wing in these animals contains abnormally large and multinucleate cells.

(Shandala et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, pbl^RNAi.UAS has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by Cdc14^GS9041, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by sti^GS9053/Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^en.PU, pbl^RNAi.UAS has visible phenotype, suppressible by Cele\ego-1^UAS.cDa, Scer\GAL4^en.PU

(Duan et al., 2013)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible by Pdk1^GS10133, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible by Diap1^GS9038, Scer\GAL4^en-e16E

(Gregory et al., 2007)

NOT suppressed by

Statement

Reference

Scer\GAL4^en.PU, pbl^RNAi.UAS has visible phenotype, non-suppressible by Cele\ego-1^UASp.cDa, Scer\GAL4^en.PU

(Duan et al., 2013)

Scer\GAL4^en.PU, pbl^RNAi.UAS has visible phenotype, non-suppressible by Cele\rrf-1^UAS.cDa, Scer\GAL4^en.PU

(Duan et al., 2013)

Other

Statement

Reference

RhoBTB^GS51, Scer\GAL4^en-e16E, pbl^RNAi.UAS has lethal phenotype

(Gregory et al., 2007)

Ide^GS51, Scer\GAL4^en-e16E, pbl^RNAi.UAS has lethal phenotype

(Gregory et al., 2007)

Rac2^GS10622, Scer\GAL4^en-e16E, pbl^RNAi.UAS has lethal | heat sensitive phenotype

(Gregory et al., 2007)

CHES-1-like^GS1023, Scer\GAL4^en-e16E, pbl^RNAi.UAS has lethal | heat sensitive phenotype

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS, wdb^GS3071 has lethal phenotype

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS, stg^GS3120 has abnormal mitotic cell cycle | third instar larval stage phenotype

(Gregory et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, enhanceable by Cdc14^GS9041, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, enhanceable by Cdc14^GS9041, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, enhanceable by Mbs^GS13173, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, enhanceable by Mbs^GS13173, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, enhanceable | heat sensitive by Rac2^GS10622, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, enhanceable | heat sensitive by Rac2^GS10622, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, enhanceable | heat sensitive by CHES-1-like^GS1023, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, enhanceable | heat sensitive by CHES-1-like^GS1023, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing phenotype, enhanceable by CycH^GS9043, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing hair | increased number phenotype, enhanceable by Df(3L)iro-2/+

(Shandala et al., 2004)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing blade posterior compartment phenotype, enhanceable by Df(3L)iro-2/+

(Shandala et al., 2004)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing hair | increased number phenotype, enhanceable by sti^KG01697/sti[+]

(Shandala et al., 2004)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing blade posterior compartment phenotype, enhanceable by sti^KG01697/sti[+]

(Shandala et al., 2004)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing hair | increased number phenotype, enhanceable by Rho1^72R/Rho1[+]

(Shandala et al., 2004)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing blade posterior compartment phenotype, enhanceable by Rho1^72R/Rho1[+]

(Shandala et al., 2004)

NOT Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, non-enhanceable by sti^GS9053/Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, non-enhanceable by sti^GS9053/Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, non-enhanceable by Cdk4^GS2301, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, non-enhanceable by Cdk4^GS2301, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, non-enhanceable by Lst^GS2301, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, non-enhanceable by Lst^GS2301, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, non-enhanceable by Scer\GAL4^en-e16E/tefu^GS13617

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, non-enhanceable by Scer\GAL4^en-e16E/tefu^GS13617

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing blade posterior compartment phenotype, non-enhanceable by Rok²/Rok[+]

(Shandala et al., 2004)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing hair | increased number phenotype, non-enhanceable by rok[+]/Rok²

(Shandala et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing blade posterior compartment phenotype, suppressible by Cele\ego-1^UAS.cDa, Scer\GAL4^en-e16E

(Duan et al., 2013)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing phenotype, suppressible | partially by Pdk1^GS10133, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing phenotype, suppressible | partially by Diap1^GS9038, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing phenotype, suppressible | partially by CycH^GS9043, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, suppressible | partially by stg^GS3120/Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, suppressible | partially by Scer\GAL4^en-e16E/wun^GS10009

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, suppressible | partially by Scer\GAL4^en-e16E/aurA^GS7173

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, suppressible | partially by Pitslre^GS10080, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, suppressible | partially by Diap1^GS9038, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, suppressible | partially by stg^GS3120/Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, suppressible | partially by Scer\GAL4^en-e16E/wun^GS10009

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, suppressible | partially by Scer\GAL4^en-e16E/aurA^GS7173

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, suppressible | partially by Pitslre^GS10080, Scer\GAL4^en-e16E

(Gregory et al., 2007)

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing blade posterior compartment phenotype, non-suppressible by Cele\ego-1^UASp.cDa, Scer\GAL4^en-e16E

(Duan et al., 2013)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing blade posterior compartment phenotype, non-suppressible by Cele\rrf-1^UAS.cDa, Scer\GAL4^en-e16E

(Duan et al., 2013)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, non-suppressible by Cdk4^GS2301, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, non-suppressible by Lst^GS2301, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, non-suppressible by Scer\GAL4^en-e16E/tefu^GS13617

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior phenotype, non-suppressible by sti^GS9053/Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, non-suppressible by Cdk4^GS2301, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, non-suppressible by Lst^GS2301, Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, non-suppressible by Scer\GAL4^en-e16E/tefu^GS13617

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing | posterior & wing hair phenotype, non-suppressible by sti^GS9053/Scer\GAL4^en-e16E

(Gregory et al., 2007)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing hair | increased number phenotype, non-suppressible by sti^GS9053/Scer\GAL4^en-e16E

(Shandala et al., 2004)

Scer\GAL4^en-e16E, pbl^RNAi.UAS has wing blade posterior compartment phenotype, non-suppressible by sti^GS9053/Scer\GAL4^en-e16E

(Shandala et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rac2^GS10622 or CHES-1-like^GS1023 in a Scer\GAL4^en-e16E, pbl^{dsRNA.Scer\UAS} background results in lethality at 25^oC and enhancement of the wing phenotype at 18^oC.

Co-expression of Pk61C^GS10133 suppresses both the G2 and hyperploid cell effects seen in Scer\GAL4^en-e16E, pbl^{dsRNA.Scer\UAS} wing discs.

Co-expression of cdc14^GS9041 strongly enhances the hyperploid cell fraction seen in Scer\GAL4^en-e16E, pbl^{dsRNA.Scer\UAS} wing discs and reduces the fraction of cells in G1 phase.

Co-expression of th^GS9038 reduces the proportion of hyperploid cells seen in Scer\GAL4^en-e16E, pbl^{dsRNA.Scer\UAS} wing discs.

Co-expression of stg^GS3120 does not significantly affect the hyperploid cell effects seen in Scer\GAL4^en-e16E, pbl^{dsRNA.Scer\UAS} wing discs, though it does strongly push cells from the G2 to the G1 fraction.

(Gregory et al., 2007)

The reduction in compartment size and the percentage of cells with multiple wing hairs in pbl^{dsRNA.Scer\UAS}; Scer\GAL4^en-e16E flies are both enhanced by Rho1^72R/+, Df(3L)iro-2/+ or sti^KG01697/+, but not by rok²/+. These phenotypes are not suppressed by sti^GS9053 (note that while this is this a loss of function allele, in the presence of Scer\GAL4 it drives ectopic expression of sti.)

(Shandala et al., 2004)

Xenogenetic Interactions

Statement

Reference

Co-expression of either Cele\rrf-1^Scer\UAS.cDa or Cele\ego-1^Scer\UAS.P\T has no effect on the defect seen in the posterior compartment of the wing in animals expressing pbl^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en.PU.

Co-expression of Cele\ego-1^Scer\UAS.cDa suppresses the defect seen in the posterior compartment of the wing in animals expressing pbl^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en.PU.

(Duan et al., 2013)

Co-expression of BacA\p35^Scer\UAS.cHa enhances the proportion of hyperploid cells seen in Scer\GAL4^en-e16E, pbl^{dsRNA.Scer\UAS} wing discs.

(Gregory et al., 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pbl^RNAi.UAS

pbl^{dsRNA.Scer\UAS}

pbl^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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