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General Information
Symbol
Dmel\robo2dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0179063
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-robo2-RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a robo2 RNAi construct.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

robo2dsRNA.Scer\UAS knockdown MARCM clones (in a female Scer\GAL4fru-NP0021 mAL cluster) do not develop a male-specific ipsilateral ectopic neurite (as seen in robo1GD2608 knockdown clones).

The expression of robo2dsRNA.Scer\UAS under the control of Scer\GAL4Pdf.PU does not significantly affect the length of sLNv axons compared to controls.

The expression of robo2dsRNA.Scer\UAS under the control of Scer\GAL4Mef2.247 leads to significant decreases in migration velocity, as well as in filopodial and lamellopodial extensions in embryonic heart cardioblasts, as compared to controls.

Females expressing leadsRNA.Scer\UAS under the control of Scer\GAL4Poxn.14 do not show midline crossing by foreleg gustatory receptor neuron (GRN) axons (this phenotype is the same as in wild-type females). Males expressing leadsRNA.Scer\UAS under the control of Scer\GAL4Poxn.14 show reduced midline crossing by foreleg GRNs compared to that seen in control males.

Expression of a single copy of leadsRNA.Scer\UAS under the control of Scer\GAL4elav-C155 or Scer\GAL4sca-375.1 has no effect on visual system development.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of robo2dsRNA.Scer\UAS and robo3dsRNA.Scer\UAS, but not of robo2dsRNA.Scer\UAS and robo1dsRNA.Scer\UAS, under the control of Scer\GAL4Pdf.PU results in adult sLNv neurons exhibiting significantly longer axon projections compared to controls.

Males and females simultaneously co-expressing robodsRNA.Scer\UAS, leadsRNA.Scer\UAS and robo3dsRNA.Scer\UAS under the control of Scer\GAL4Poxn.14 show severe disruption of foreleg gustatory receptor neuron axon morphology, with axons collapsing onto the midline.

Simultaneous co-expression of robodsRNA.Scer\UAS, leadsRNA.Scer\UAS and robo3dsRNA.Scer\UAS under the control of Scer\GAL4elav-C155 results in defects in visual system development; distal cell neurons invade the developing lamina. Simultaneous co-expression of robodsRNA.Scer\UAS, leadsRNA.Scer\UAS and robo3dsRNA.Scer\UAS under the control of Scer\GAL4sca-375.1 results in distal cell neurons invading the developing lamina. The distal cell neurons and lamina glia are intermingled in these animals. In addition defects in photoreceptor axon targeting are seen, with the regions of mistargeting correlating with the regions where the distal cell neurons enter the lamina. Simultaneous co-expression of robodsRNA.Scer\UAS, leadsRNA.Scer\UAS and robo3dsRNA.Scer\UAS under the control of Scer\GAL4αTub84B.PL results in many photoreceptor axons extending through the lamina and too many photoreceptor axons entering the medulla. Simultaneous co-expression of robodsRNA.Scer\UAS, leadsRNA.Scer\UAS and robo3dsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in any defects in photoreceptor axon targeting.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
leadsRNA.Scer\UAS
robo2dsRNA.Scer\UAS
robo2dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (7)