Allele Dmel\lea^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\lea^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0179063
Feature type	allele	Associated gene	Dmel\lea

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Tayler et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a lea RNAi construct. (Tayler et al., 2004)
Carried in construct	P{UAS-lea.RNAi} (Tayler et al., 2004, Mellert et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| adult stage \| male, with Scer\GAL4^Poxn.14 (Mellert et al., 2010)
Phenotype Manifest In
	prothoracic leg taste bristle chemosensory neuron \| male, with Scer\GAL4^Poxn.14 (Mellert et al., 2010)
Detailed Description
	Statement Reference Females expressing lea[dsRNA.Scer\UAS] under the control of Scer\GAL4[Poxn.14] do not show midline crossing by foreleg gustatory receptor neuron (GRN) axons (this phenotype is the same as in wild-type females). Males expressing lea[dsRNA.Scer\UAS] under the control of Scer\GAL4[Poxn.14] show reduced midline crossing by foreleg GRNs compared to that seen in control males. (Mellert et al., 2010) Expression of a single copy of lea^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-375.1 has no effect on visual system development. (Tayler et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Scer\GAL4^elav-C155, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has neuroanatomy defective phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^sca-375.1, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has neuroanatomy defective phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^αTub84B.PL, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has neuroanatomy defective phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004)
Phenotype Manifest In
Other
Statement Reference Scer\GAL4^elav-C155, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has lamina anlage phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^Poxn.14, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has prothoracic leg taste bristle chemosensory neuron \| male phenotype (Mellert et al., 2010) Scer\GAL4^sca-375.1, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has eye photoreceptor cell & axon phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^sca-375.1, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has lamina anlage phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004) Scer\GAL4^αTub84B.PL, lea^{dsRNA.Scer\UAS}, robo3^{dsRNA.Scer\UAS}, robo^{dsRNA.Scer\UAS} has eye photoreceptor cell & axon phenotype (Tayler et al., 2004, Tayler et al., 2004, Tayler et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Males and females simultaneously co-expressing robo[dsRNA.Scer\UAS], lea[dsRNA.Scer\UAS] and robo3[dsRNA.Scer\UAS] under the control of Scer\GAL4[Poxn.14] show severe disruption of foreleg gustatory receptor neuron axon morphology, with axons collapsing onto the midline. (Mellert et al., 2010) Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in defects in visual system development; distal cell neurons invade the developing lamina. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-375.1 results in distal cell neurons invading the developing lamina. The distal cell neurons and lamina glia are intermingled in these animals. In addition defects in photoreceptor axon targeting are seen, with the regions of mistargeting correlating with the regions where the distal cell neurons enter the lamina. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL results in many photoreceptor axons extending through the lamina and too many photoreceptor axons entering the medulla. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF does not result in any defects in photoreceptor axon targeting. (Tayler et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	9286 w^*; sna^Sco/CyO, P{ActGFP}JMR1; P{UAS-lea.RNAi}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	lea^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Mellert et al., 2010, Development 137(2): 323--332 Midline crossing by gustatory receptor neuron axons is regulated by fruitless, doublesex and the Roundabout receptors. [FBrf0209580] Tayler et al., 2004, Development 131(23): 5935--5945 Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins. [FBrf0180181]

Allele Dmel\leadsRNA.Scer\UAS

Allele Dmel\lea^{dsRNA.Scer\UAS}