FlyBase Allele Report: Dmel\hpo[RNAi.UAS.cPa]

General Information

Symbol

Dmel\hpo^RNAi.UAS.cPa

Species

D. melanogaster

Name

FlyBase ID

FBal0179448

Feature type

allele

Associated gene

Dmel\hpo

Associated Insertion(s)

Carried in Construct

P{UAS-RNAi-hpo.P}

Key Links

Transgenic product class

RNAi_reagent

(Pantalacci et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Pantalacci et al., 2003)

Mutagen

in vitro construct

(Pantalacci et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-RNAi-hpo.P}

Cytology

Description

UAS sequences are cloned upstream of the C-terminal 1138-2514 nucleotides of hpo used to generate dsRNA within the cell.

(Pantalacci et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

hpo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Irwin et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye | pupal stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Das Thakur et al., 2010)

eye-antennal disc, with Scer\GAL4^Act5C.PP

(Pantalacci et al., 2003)

haltere, with Scer\GAL4^Act5C.PP

(Pantalacci et al., 2003)

imaginal disc, with Scer\GAL4^Act5C.PP

(Pantalacci et al., 2003)

interommatidial cell | pupal stage, with Scer\GAL4^αTub84B.PL

(Das Thakur et al., 2010)

wing | posterior, with Scer\GAL4^en-e16E

(Poon et al., 2011)

wing hinge primordium, with Scer\GAL4^sd-SG29.1

(Fernández et al., 2011)

Detailed Description

Statement

Reference

Expression of hpo^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sd-SG29.1 results in an expansion (by 55%) of the distal hinge domain of the wing disc.

(Fernández et al., 2011)

Expression of hpo^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E results in wing overgrowth.

(Poon et al., 2011)

Pupal eye clones of cells expressing hpo^{dsRNA.Scer\UAS} driven by Scer\GAL4^αTub84B.PL exhibit increased clonal area as well as overproliferation of interommatidial cells.

(Das Thakur et al., 2010)

hpo^{dsRNA.Scer\UAS} expression in somatic clones induces an increase in tissue and organ size, leading, for example, to oversized halteres, or to an overgrowth of imaginal discs. Photoreceptor differentiation in the third instar larval eye imaginal disc is unaffected by hpo^{dsRNA.Scer\UAS} induction, however, third instar eye imaginal discs containing large flp-out hpo^{dsRNA.Scer\UAS} clones are enlarged compared to wild-type. Cells in larval eye imaginal disc hpo^{dsRNA.Scer\UAS} clones fail to arrest in the morphogenetic furrow and, after the second mitotic wave, undergo additional rounds of division.

(Pantalacci et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, hpo^RNAi.UAS.cPa has abnormal size | adult stage phenotype, enhanceable by Tao⁵⁰

(Poon et al., 2011)

Scer\GAL4^en-e16E, hpo^RNAi.UAS.cPa has visible | adult stage phenotype, enhanceable by Tao⁵⁰

(Poon et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PP/hpo^RNAi.UAS.cPa is a suppressor of increased cell death phenotype of hid^GMR.PG

(Pantalacci et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, hpo^RNAi.UAS.cPa has wing | posterior phenotype, enhanceable by Tao⁵⁰

(Poon et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^αTub84B.PL, hpo^RNAi.UAS.cPa has eye | somatic clone | pupal stage phenotype, suppressible by Df(1)jub^I, Scer\GAL4^αTub84B.PL

(Das Thakur et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Tao⁵⁰ enhances the increase in wing size seen when hpo^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^en-e16E.

(Poon et al., 2011)

Homozygous mutant clones of Df(1)djub^I also expressing wts^GD1563 driven by Scer\GAL4^αTub84B.PL display a Df(1)djub^I-like phenotype of decreased clonal area.

(Das Thakur et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

UAS-RNAi-hpo

(Pantalacci et al., 2003)

hpo^RNAi.UAS.cPa

hpo^RNAi.UAS

hpo^RNAi

(Forest et al., 2018)

hpo^{dsRNA.Scer\UAS}

hpo^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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