FB2025_01 , released February 20, 2025
Allele: Dmel\fwdZ0453
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General Information
Symbol
Dmel\fwdZ0453
Species
D. melanogaster
Name
FlyBase ID
FBal0179743
Feature type
allele
Associated gene
Dmel\fwd
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Giansanti et al., 2007)
Mutagen
ethyl methanesulfonate
(Giansanti et al., 2004)
Progenitor genotype
Cytology
Description

Amino acid replacement: S1318L.

(Giansanti et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:306,423..306,423 [-]
Nucleotide change:

C306423T

Amino acid change:

S1654L | fwd-PA; S1654L | fwd-PB; S1318L | fwd-PC; S1654L | fwd-PD; S1654L | fwd-PE

Reported amino acid change:

S1318L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Giansanti et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      contractile ring & meiotic telophase I | male (with Df(3L)7C)

      (Giansanti et al., 2004)

      contractile ring & meiotic telophase II | male (with Df(3L)7C)

      (Giansanti et al., 2004)

      Nebenkern

      (Giansanti et al., 2004)

      spermatid (with fwd3)

      (Giansanti et al., 2007)

      spermatid & nucleus | supernumerary

      (Giansanti et al., 2004)

      spindle & meiotic telophase I | male (with Df(3L)7C)

      (Giansanti et al., 2004)

      spindle & meiotic telophase II | male (with Df(3L)7C)

      (Giansanti et al., 2004)
      Detailed Description
      Statement
      Reference

      fwd3/fwdZ0453 spermatids are multinucleated.

      (Giansanti et al., 2007)

      Analysis of mutant spermatocytes from fwdZ0453/Df(3L)7C flies reveals that they exhibit an equatorial localisation of Golgi vesicles in 44% of the telophases.

      (Giansanti et al., 2006)

      A large proportion of fwdZ0453 mutant spermatids have one large nebenkern with four nuclei of normal size, indicating a cytokinetic failure in both meiotic divisions, while a smaller proportion have two nuclei and a large nebenkern, indicating failure of only one of the meiotic divisions. Occasionally spermatids are seen (<1% of cases) with three nuclei, which may be due to failure of cytokinesis in the first meiotic division, followed by budding off of only one daughter cell in the second meiotic division. Further, some spermatids contain more than four nuclei, which may result from defects in the mitotic divisions preceding mitosis. Additionally, a small proportion of spermatids contain nuclei of irregular sizes. During early telophase of fwdZ0453/Df(3L)7C spermatocytes, there is an actin ring of normal appearance and and a normal central spindle. However, as telophase progresses the actin ring often fails to fully constrict and in late telophases where there is a high degree of constriction, there is a greater amount of F-actin at the ring. In a small proportion of late telophase figures, the central spindle is also defective.

      (Giansanti et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      fwdZ0453/fwd3 has spermatid phenotype, non-enhanceable by Rab1193Bi/Rab11ETo11

      (Giansanti et al., 2007)

      fwdZ0453/fwd3 has spermatid phenotype, non-enhanceable by vibEP513

      (Giansanti et al., 2007)
      NOT suppressed by
      Statement
      Reference

      fwdZ0453/fwd3 has spermatid phenotype, non-suppressible by Rab1193Bi/Rab11ETo11

      (Giansanti et al., 2007)

      fwdZ0453/fwd3 has spermatid phenotype, non-suppressible by vibEP513

      (Giansanti et al., 2007)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Testes from bruZ0704/bruZ3358, fwdZ0453/Df(3L)7C males produce higher percentages of multi-nucleate early spermatids associated with enlarged mitochondrial derivatives than testes from siblings containing one mutant and one wild type copy of either bru or fwd.

      The elongation of later-stage spermatids is defective in bruZ0704/bruZ3358, fwdZ0453/Df(3L)7C testes.

      Mitotic cytokinesis appears normal in bruZ0704/bruZ3358, fwdZ0453/Df(3L)7C third instar larval brains.

      (Robinett et al., 2009)

      Rab1193Bi/Rab11ETo11, fwd3/fwdZ0453 spermatids are multinucleated at a similar frequency to fwd3/fwdZ0453 spermatids.

      vibEP513, fwd3/fwdZ0453 spermatids are multinucleated at a similar frequency to fwd3/fwdZ0453 spermatids.

      (Giansanti et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      fwdZ0453
      (Giansanti et al., 2007, Giansanti et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)