E534K | eya-PA; E528K | eya-PB; E444K | eya-PC
Analogous mutation in human EYA1 implicated in branchiootorenal syndrome 1; mutation carried on in vitro construct.
UASt regulatory sequences drive expression of a mutated form of eya (carries the amino acid replacement E528K) that is tagged at the N-terminal end with Tag:FLAG.
Eye defects are seen in flies when eyaE528K.Scer\UAS.T:Zzzz\FLAG is expressed in a eya2 mutant background.
visible, with Scer\GAL4dpp.blk1
eye | ectopic, with Scer\GAL4dpp.blk1
Expression of eyaE528K.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4dpp.blk1 results in ectopic eye induction.
eyaE528K.UAS.Tag:FLAG/Scer\GAL4dpp.blk1 partially rescues eya2
Expression of eyaE528K.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4dpp.blk1 significantly rescues eye tissue formation in eya2 flies.